Phospho-MAPK3 (Tyr204) Antibody

Code CSB-PA089297
Size US$360
  • Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide (right).
  • Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody.
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) MAPK3 Polyclonal antibody
Uniprot No. P27361
Target Names MAPK3
Alternative Names ERK 1 antibody; ERK-1 antibody; ERK1 antibody; ERT 2 antibody; ERT2 antibody; Extracellular Signal Regulated Kinase 1 antibody; Extracellular signal related kinase 1 antibody; Extracellular signal-regulated kinase 1 antibody; HGNC6877 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin Stimulated MAP2 Kinase antibody; Insulin-stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 3 antibody; MAP Kinase antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 3 antibody; MAPK antibody; MAPK1 antibody; Mapk3 antibody; MGC20180 antibody; Microtubule Associated Protein 2 Kinase antibody; Microtubule-associated protein 2 kinase antibody; Mitogen Activated Protein Kinase 3 antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 3 antibody; MK03_HUMAN antibody; OTTHUMP00000174538 antibody; OTTHUMP00000174541 antibody; p44 ERK1 antibody; p44 MAPK antibody; p44-ERK1 antibody; p44-MAPK antibody; P44ERK1 antibody; P44MAPK antibody; PRKM 3 antibody; PRKM3 antibody; Protein Kinase Mitogen Activated 3 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase.
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration It differs from different batches. Please contact us to confirm it.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC,IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
IF 1:100-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Gene References into Functions
  1. Data suggest that Thr264 in TRPV3 is key ERK1 phosphorylation site mediating EGFR-induced sensitization of TRPV3 to stimulate signaling pathways involved in regulating skin homeostasis. (TRPV3 = transient receptor potential cation channel subfamily V member-3; ERK1 = extracellular signal-regulated kinase-1; EGFR = epidermal growth factor receptor) PMID: 29084846
  2. RASSF7 promotes cell proliferation through activating MEK1/MEK2-ERK1/ERK2 signaling pathway in hepatocellular carcinoma. PMID: 29729697
  3. ERK1 Directly Interacts With JNK1 Leading to Regulation of JNK1/c-Jun Activity and Cell Transformation. PMID: 28106280
  4. the D domain of LRRC4 anchors ERK1/2 in the cytoplasm and competitively inhibits MEK/ERK activation in glioma cells. PMID: 27884160
  5. High ERK1 expression is associated with castration-resistant prostate cancer. PMID: 28844715
  6. The antitumor activity of scopoletin may be due to its strong anti-angiogenic effect, which may be mediated by its effective inhibition of ERK1, VEGF-A, and FGF-2. PMID: 27133199
  7. High ERK1 expression is associated with melanoma. PMID: 28193911
  8. findings uncover a role of ERK1 in the regulation of furin activity by supporting a self-sustaining loop for high TGF-beta activity in glioma-initiating cells. PMID: 28484053
  9. ERK1 phosphorylation is mediated by Src and Csk. PMID: 26234813
  10. Integrin beta1 appears to serve as a partner of Stathmin induction of ERK and Akt signaling by inhibiting apoptosis in the cholangiocarcinoma cell. PMID: 28178656
  11. High ERK1 expression is associated with gastric cancer. PMID: 27601158
  12. Data indicate three biomarkers mitogen-activated protein kinase 3 (MAPK3), BCL2 apoptosis regulator (BCL2) and proto-oncogene c-Akt (AKT1) as potential predictors of neurological outcome following cardiac arrest (CA). PMID: 28147324
  13. Inhibiting miR-21 attenuates hepatic fibrosis by suppressing both the ERK1 in hepatic stellate cells and epithelial-mesenchymal transition of hepatocyte. PMID: 27226339
  14. High ERK1 expression is associated with paclitaxel resistance in cervical cancer. PMID: 26810068
  15. High ERK1 expression is Associated with Nucleus Pulposus Cells' Degeneration. PMID: 27635110
  16. Low expression of ERK is associated with resistance to sorafenib in liver cancer. PMID: 26711788
  17. Increased ERK1 expression is associated with drug resistance in neoplasms. PMID: 26715278
  18. The activation of ERK1 by MEK1, subsequent slower phosphorylation of the flanking sites results in inhibition of the kinase. Because the T207 and Y210 phosphosites of ERK1 are highly conserved within the eukaryotic protein kinase family, hyperphosphorylation within the kinase activation T-loop may serve as a general mechanism for protein kinase down-regulation after initial activation by their upstream kinases. PMID: 26823016
  19. Over-expressed TWIST associates with markers of epithelial mesenchymal transition and predicts poor prognosis in breast cancers via ERK and AKT activation. PMID: 26295469
  20. p44/42, a known apoptosis-promoting regulator and caspase 3 activator, was increased in brain tumor cells treated with violacein PMID: 25816226
  21. miR-155 plays an important role in regulating the pathological network involving EMT process and ERK1 pathway during hepatic stellate cell activation. PMID: 25142507
  22. This study identified and confirmed MAPK3 protein changes within the postsynaptic density in schizophrenia PMID: 25048004
  23. Sphingosine-1-phosphate promotes extravillous trophoblast cell invasion by activating MEK/ERK/MMP-2 signaling pathways via S1P/S1PR1 axis activation. PMID: 25188412
  24. Results show that miR-483-5p expression level is up-regulated in polycystic ovary syndrome patients and inversely correlated with notch3 and MAPk3 levels and that Notch3 and MAPK3 are the direct targets of MIR483. PMID: 25622783
  25. SKLB-M8 inhibited HUVEC proliferation, migration, invasion, and tube formation in vitro with the inhibition of phosphorylated ERK1/2. PMID: 25341684
  26. Therefore, the positivity of p-ERK1/2 expression may serve as a vital biomarker in the development of non-small cell lung cancer PMID: 25596700
  27. Low ERK1 expression is associated with hormone resistance in breast cancer. PMID: 25085753
  28. Therefore, our study demonstrates that MAPK members (ERK1/2 and JNK) play a key role in CCR7 regulating SCCHN metastasis PMID: 25270024
  29. High phosphorylated ERK1 is associated with low response to chemotherapy in nonsmall-cell lung carcinoma. PMID: 25449334
  30. These results therefore indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype through dephosphorylating ERK1/2 PMID: 25414256
  31. ERK1/2 signal induced MNK catalytic activity enabled enterovirus type 1 internal ribosomal entry site-mediated translation/host cell cytotoxicity through negative regulation of the Ser/Arg (SR)-rich protein kinase (SRPK). PMID: 25187541
  32. ERK, AKT, and GSK-3beta have roles in boldine-induced cell cycle arrest and apoptosis in T24 human bladder cancer cell line PMID: 24239461
  33. Insulin-induced apoptotic commitment depended on the down-regulation of Erk-1, insulin growth factor-1 receptor (IGF-1R), and fibroblast growth factor receptor-1 (FGFR-1)-mediated signaling. PMID: 24818995
  34. Enhanced t-ERK1 expression in infiltrating lymphoid cells was significantly associated with female gender, absence of vascular and perineural invasion, lymph node metastases and early depth of invasion as well as with longer disease-free survival times PMID: 24682903
  35. Expression of TMPRSS4 in gastric cancer is significantly associated with lymph node and distant metastasis, high Erk1 expression, and poor prognosis. PMID: 23922976
  36. ERK1 phosphorylates KIBRA at Ser(548) for cell proliferation and migratory activity PMID: 24269383
  37. We conclude that pERK1/2 is a sensitive marker of early colon cancer, which disappears at later stages of cancer development. PMID: 23357054
  38. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
  39. ERK1 localized to the cytosol and translocated to the nucleus upon cell activation and kinase phosphorylation. PMID: 23651922
  40. Data indicate that tocilizumab enhanced the interferon-induced phosphorylation of STAT1 and inhibited SOCS3 expression and the phosphorylation of both STAT3 and ERK. PMID: 23274199
  41. Extracellular signal-regulated kinase and glycogen synthase kinase 3beta regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism PMID: 23408424
  42. ERK1/2 signaling plays an important role in topoisomerase II poison-induced G2/M checkpoint activation. PMID: 23166842
  43. MRK is a novel RhoC effector that controls LPA-stimulated cell invasion at least in part by regulating myosin dynamics, ERK and p38 PMID: 23319595
  44. osteosarcoma patients whose tumors expressed pERK1 had a poorer clinical outcome than those whose tumors did not. PMID: 22935974
  45. Results showed significantly higher levels of ERK1 protein in smokers vs. non-smokers. Analysis revealed a significant relation among the number of cigarettes smoked daily, the Fagerstrom Test for Nicotine Dependence score and the mRNA expression of ERK1. PMID: 21070506
  46. constitutive activation of the ERK1 pathway in HER2/ERBB2-transformed cells prevents EGF deprivation-induced FLIPL upregulation and TRAIL resistance. PMID: 22722337
  47. Lead specifically induces dysregulation of iron response element (IRP)1 protein by activating the ERK1/2 signaling pathway, indicating a novel role for IRP1 and the ERK/MAPK pathway in vascular endothelial functions. PMID: 22502979
  48. CXCL12/CXCR4 protein signaling axis induces sonic hedgehog expression in pancreatic cancer cells via extracellular regulated kinase- and Akt kinase-mediated activation of nuclear factor kappaB PMID: 22995914
  49. Aortic endothelial cells stimulated with HLA class I antibodies did not promote any detectable change in intracellular Ca(2+) concentration but instead induced MLC phosphorylation and stress fiber assembly. PMID: 22914643
  50. The inhibition of Id-1 expression by MK615 is mediated via ERK1/2 activation. PMID: 22076920
  51. caspase-3 cleavage of MKP3 down-regulates MKP3 full length and renders active MKP3 fragments, which may participate in novel regulatory pathways controlling the subcellular localization and activation of ERK1/2 during apoptosis. PMID: 22504224
  52. Suggest that in pancreatic ductal carcinoma ERK1 expression correlated to shorter postsurgical recurrence-free and overall survivals. PMID: 22158075
  53. EBV-encoded LMP1 upregulates Igkappa 3'enhancer activity and Igkappa expression in nasopharyngeal cancer cells by activating the Ets-1 through ERKs signaling PMID: 22396784
  54. miR-483-5p can serve as a tumor suppressor in gliomas PMID: 22465663
  55. Phospho-ERK and AKT status, but not KRAS mutation status, are associated with outcomes in rectal cancer treated with chemoradiotherapy PMID: 21910869
  56. Oxidized low density lipoprotein induces cyclin A synthesis in a process involving ERK, JNK and NFkappaB PMID: 21764057
  57. cAMP induces autophagy via a novel pathway involving ERK, cyclin E and Beclin 1 PMID: 21750416
  58. mp53 disrupts the role of ShcA in balancing the Smad-dependent and -independent signaling activity of TGF-beta and ShcA/ERK signaling is a major pathway regulating the tumor-promoting activity of TGF-beta PMID: 22039050
  59. Imbalance in PRA/PRB ratio frequently associated with carcinogenesis might be a direct consequence of disorders in MAPK signaling. PMID: 21816898
  60. It is suggested that since nuclear pERK1/2 can be mitogenic, it can serve as a reliable marker for the progression of ovarian cancer PMID: 21687944
  61. Cell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK PMID: 21952366
  62. Data show that Fas-mediate events are mediated by ERK/JNK MAPKs which subsequently activate NF-kappaB for the transcriptional activation of pro-inflammatory mediators PMID: 21752354
  63. human rhabdomyosarcoma RD cell differentiation is regulated by reciprocal, functional interactions between myostatin, p38 and extracellular regulated kinase signalling pathways PMID: 21273059
  64. Functional and molecular interactions between ERK and CHK2 in diffuse large B-cell lymphoma. PMID: 21772273
  65. The up-regulation of ERK1/2-dependent thymidylate synthase and thymidine phosphorylase can protect human lung cancer cells from cisplatin-induced cytotoxicity. PMID: 21444628
  66. the PI3K/PAK1/ERK signaling pathway has a role in LPA-stimulated breast cancer cell migration PMID: 21209852
  67. Overall results proved downregulation of ERK1 by ubiquitin proteasomes and suppression of interaction between ERK1 and STAT1 as type I IFN antagonist function of SARS-CoV PLpro. PMID: 21270289
  68. Study demonstrates a novel role of the ERK1/2 signaling pathway in promoting myocardium inflammation and dysfunction in coronary microembolization. PMID: 21150201
  69. Both PAR2 and TLR4 activation on SW620 cells can trigger the phosphorylation of ERK1/2, regulate gene expression, and promote the proliferation and migration of cells. PMID: 21152870
  70. Gene knockdown of ERK1 resulted in relocation of ATR from the nucleoplasm to the nucleolus in response to hydroxyurea, therefore making ATR unavailable to the sites of DNA damage. PMID: 20840867
  71. Human cells from bronchus, intestine, and skin (keratinocytes)showed similar time patterns for the activation of mitogen-activated protein kinases Erk1/2 when treated with carbon nanoparticles. PMID: 20868224
  72. Increased expression of phosphorylated ERK1 is associated with T4 breast carcinoma. PMID: 20735841
  73. Knockdown of ERK1 kinase reduced ATM activation in response to etoposide treatment, and thereby attenuated phosphorylation of the ATM substrates. PMID: 20637859
  74. Data show that blockade of amphiregulin suppressed the activities of EGFR, HER3, and Akt and the expression of amphiregulin itself. PMID: 20726858
  75. SH3GL2 participates in the regulation of apoptosis through the MEK-ERK signal pathway by adjusting EGFR in the laryngeal carcinoma cell line Hep2 PMID: 20512084
  76. MAPK3 is an important initiating event in Nef-mediated ICAM-1 expression in Nef-expressed cells. PMID: 20566577
  77. ERK-1 kinase prevents TNF + cycloheximide induced apoptosis by regulating the intrinsic mitochondrial pathway through different mechanisms in HeLa cells. PMID: 19777442
  78. These results suggest that in human tracheal smooth muscle cells, activation of MAPKs, NF-kappaB, and p300 are essential for IL-1beta-induced cPLA(2) expression and PGE(2) secretion. PMID: 20069553
  79. bFGF regulates ERK oscillations in premalignant but not malignant JB6 cells PMID: 20016498
  80. Restoration of chemosensitivity in ovarian cancer cells in 3-d cultures by inhibition of c-Met was dependent on extracellular signal-regulated kinase (ERK) 1/2 signaling pathways PMID: 20126471
  81. The authors observed that extracellular signal-regulated kinase (ERK) activation promotes viral fusion via ezrin-mediated cytoskeletal rearrangements, whereas AKT1 attenuates fusion by promoting phosphorylation of F protein of Sendai virus. PMID: 20164223
  82. The differential expression of the Mitf 6a/b isoforms is dependent on the MAPKinase signalling, being linked to the activation of MEK1-ERK2, but not to N-RAS/B-RAF mutation status. PMID: 19895547
  83. Bcr-Abl represses the expression of RKIP, continuously activates pERK1/2, and suppresses FoxM1 expression, resulting in proliferation of CML cells. PMID: 20028985
  84. Stretch activates myometrium via ERK, caldesmon and focal adhesion signaling PMID: 19834610
  85. analysis of structural interactions of hERK1 with mitochondria in HeLa cells PMID: 19847302
  86. Furthermore, colostrum treatment increases the levels of tyrosine phosphorylated proteins and the activated forms of the extracellular signal-regulated kinases 1 and 2 in HaCaT cell line. PMID: 19919532
  87. Oligomeric aggregates of Abeta42 are the principal structural form of the peptide that activates ERK/MAPK in SH-SY5Y cells and these effects are mediated by the alpha7 nAChR. PMID: 19666073
  88. role in acidic extracellular pH in inducing VEGF in human glioblastoma cells PMID: 11741977
  89. CD40 ligation induces macrophage IL-10 and TNF-alpha production: differential use of the PI3K and p42/44 MAPK-pathways. PMID: 11792123
  90. Protease activity, growth factor-like and kringle domains of uPA differentially contribute to activation of p42/p44erk1,2 and p38 MAP-kinases. PMID: 11930938
  91. Activation of the p44 MAPK pathway contributes to the underlying mechanism of IL-12 suppression by soluble CD40 ligand. PMID: 11937531
  92. ERK1 activity ad dual-phosphorylation were undetectable in expanding and self-renewing hematopoietic progenitors (HP). Adding IL-3, inducing maturation and cell death in HP, led to sustained high levels of ERK1 activity and dual-phosphorylation. PMID: 12032872
  93. Role of ERK1 in BRCA1-induced apoptosis PMID: 12082091
  94. expression of extracellular signal-regulated kinase, ERK1, and its relationship with clinicopathological characteristics of breast cancer PMID: 12408764
  95. Helicobacter pylori-induced ERK1 activation, especially by the cagA(+) strain, may play a protective role against gastric epithelial cell apoptosis partially through maintenance of bcl-2 gene expression PMID: 12540563
  96. NiCl2 induced that of p44/42 extracellular signal-regulated kinases, p38, and stress-activated protein kinase/c-jun N-terminal kinases. PMID: 12603851
  97. ERK1 is activated in a pathway that involves MKP1 and ubiquitin-proteasome PMID: 12676937
  98. the active phosphorylated form of MAPK/ERK is aberrantly expressed in cultured and primary HD cells PMID: 12689928
  99. Results suggest that receptor activator of NF-kappaB ligand (RANKL) induction by high extracellular calcium might not be mediated by the G protein-coupled calcium-sensing receptor, but by the p44/42 mitogen-activated protein kinase pathway. PMID: 12727216
  100. Lipopolysaccharide-induced matrix metalloproteinase (MMP)-1 and MMP-9 production by monocytes is regulated by ERK1 through a mechanism involving the activation of additional MMP promoter sites via a PGE2-independent mechanism. PMID: 12794156

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Subcellular Location Cytoplasm, Nucleus, Membrane, caveola
Protein Families Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

HGNC: 6877

OMIM: 601795

KEGG: hsa:5595

STRING: 9606.ENSP00000263025

UniGene: Hs.861

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