Recombinant Human Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3)

Code CSB-YP852884HU
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Source Yeast
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Code CSB-EP852884HU
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Source E.coli
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Code CSB-EP852884HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP852884HU
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Source Baculovirus
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Code CSB-MP852884HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIK3R3
Uniprot No.
Alternative Names
DKFZp686P05226; FLJ41892; OTTHUMP00000009783; OTTHUMP00000009786; p55; p55 gamma; P55G_HUMAN; p55PIK; Phosphatidylinositol 3 kinase regulatory subunit gamma; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma); Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase regulatory subunit gamma; Phosphoinositide 3 kinase regulatory subunit 3 (gamma); Phosphoinositide 3 kinase regulatory subunit 3; Phosphoinositide 3 kinase regulatory subunit polypeptide 3; Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma); Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma); PI3 kinase p85 subunit gamma; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; Pik3r3; PtdIns 3 kinase p85 gamma; PtdIns-3-kinase regulatory subunit gamma; PtdIns-3-kinase regulatory subunit p55-gamma
Species
Homo sapiens (Human)
Expression Region
1-461
Target Protein Sequence
MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPMTSAVP NGMKDSSVSL QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG KYGFSDPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKK LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKEY IERFRREGNE KEIERIMMNY DKLKSRLGEI HDSKMRLEQD LKNQALDNRE IDKKMNSIKP DLIQLRKIRD QHLVWLNHKG VRQKRLNVWL GIKNEDADEN YFINEEDENL PHYDEKTWFV EDINRVQAED LLYGKPDGAF LIRESSKKGC YACSVVADGE VKHCVIYSTA RGYGFAEPYN LYSSLKELVL HYQQTSLVQH NDSLNVRLAY PVHAQMPSLC R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Gene References into Functions
  1. Results showed that PIK3R3 was upregulated in colorectal cancer (CRC) tissues and inversely correlated with miR-411 expression. Its expression is regulated by miR-411 which targets its 3'UTR. PMID: 29207180
  2. PIK3R3 could promote the metastasis of Pancreatic Cancer by facilitating ZEB1 induced Epithelial-Mesenchymal Transition. PMID: 29719293
  3. Using case-control study and functional analyses, study have identified SNP rs7536272 A > G, located in the promoter region of PIK3R3, acts as a risk factor of GC through enhancing the transcriptional activity of PIK3R3, contributing to increased expression of PIK3R3. PMID: 29802999
  4. The findings suggest that miR-212 acts as a tumor suppressor in colorectal cancer by directly targeting PIK3R3. PMID: 28944887
  5. miR-181a2/181b2 prominently dampened cell-cycle progression, suppressed cell growth, and promoted apoptosis of tumor cells in vitro They also effectively impeded tumor formation and growth in vivo miR-181a2/181b2 exert the tumor suppressor ability by depressing the direct target PIK3R3 (p55gamma) and consequently modulating the PIK3R3/Akt/FoxO signaling pathway PMID: 27503199
  6. p55PIK significantly stimulated the expression of AFP by activating NF-kappaB signaling pathway in hepatocellular carcinoma cells. PMID: 28970114
  7. Study showed that PIK3R3 was a target of miR-365 and its expression was upregulated and inversely correlated with miR-365 expression in glioma tissues. PMID: 28260020
  8. PIK3R3 is a direct and functional downstream target of miR-152. PIK3R3 expression was upregulated and was inversely correlated with miR-152 expression in clinical CRC tissues. Downregulation of PIK3R3 mimicked the tumor-suppressive effects of miR-152 overexpression in CRC cells. PMID: 26820128
  9. p55PIK-PI3K signaling can contribute to imatinib resistance in gastrointestinal stromal tumors by increasing KIT expression. PMID: 26587973
  10. The proliferation, migration and invasion was decreased in ovarian SKOV3 when HOTAIR or PIK3R3 was silenced. PMID: 26826873
  11. PIK3R3 was identified as a crucial target gene of miR-132. PMID: 26252738
  12. our findings provide new insights into tumor suppression by miR-511 by negatively regulating the PIK3R3/AKT/mTOR signaling pathway PMID: 25608840
  13. p55PIK could be a substrate of activated caspase 6 during paraquat-induced apoptosis, leading to loss of original biological functions and redistribution to disturb cell cycle progression. PMID: 24130211
  14. TGF-beta/NKX2.1/PIK3R3 axis is crucial in the TGF-beta-induced inhibition of cell proliferation, and the NKX2.1/PIK3R3 axis might become a target in TGF-beta receptor-repressed lung adenocarcinoma PMID: 25371235
  15. p55gamma sequentially up-regulated p53 and p21, resulting in cell-cycle arrest in S phase; small-interfering RNA knockdown of either p53 or p21 blocked p55gamma-induced vascular smooth muscle cell growth arrest. PMID: 25388664
  16. Overexpression of PIK3R3 depends on SNAI2, inducing significant epithelial-to-mesenchymal transition (EMT). PMID: 24837077
  17. discovered that ERBB4 and S6K2 were the direct targets of miR-193a-3p and that PIK3R3 and mTOR were the direct targets of miR-193a-5p in non-small-cell lung cancer PMID: 24469061
  18. study shows that the p53/miR-148b/p55PIK axis has an important role in cell proliferation and tumor growth, and may represent a novel therapeutic target for treating cancers containing p53 mutations or losses. PMID: 24632606
  19. PIK3R3, ITGB1, ITGAL, and ITGA6, were involved in the regulation of actin cytoskeleton that link to triple-negative breast cancer migration. PMID: 24982892
  20. Containing the N24 of PI3K-p55PIK. PMID: 23904382
  21. our results show that the-PCNA interaction is important in regulating DNA synthesis and contributes to tumorigenesis. PMID: 23939377
  22. Data show that miR-7 inhibits the effects of TLR9 signaling on lung cancer cells through regulation of the PIK3R3/Akt pathway. PMID: 23135998
  23. PIK3R3 was up-regulated in gastric cancer and promoted cell cycle progression and proliferation. PMID: 22876838
  24. A fusion peptide containing the p55PIK amino terminus, TAT-N24, which not only inhibited cell proliferation, but also stimulated differentiation. PMID: 22722333
  25. IGF2-PIK3R3 signaling axis is involved in promoting the growth of a subclass of highly aggressive human glioblastomas that lack EGF receptor amplification. PMID: 17360667
  26. We have identified novel nonclassical mediators of the SREBP-1 response, including p55gamma, supporting the hypothesis that SREBP-1 regulates stress response and signaling genes. PMID: 17452746

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Protein Families
PI3K p85 subunit family
Tissue Specificity
Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Database Links

HGNC: 8981

OMIM: 606076

KEGG: hsa:8503

STRING: 9606.ENSP00000262741

UniGene: Hs.655387

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