Cathepsin O is a protein in humans that is encoded by CTSO gene. Proteolytic enzyme possibly involved in normal cellular protein degradation and turnover.
The following CTSO reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CTSO Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006203XA01HU | CTSO Antibody |
Homo sapiens (Human) | ELISA, WB (ensure identification of antigen) |
CSB-PA006203ZA01HU | CTSO Antibody |
Homo sapiens | ELISA, WB (ensure identification of antigen) |
CTSO Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP006203HU CSB-EP006203HU-B |
Recombinant Human Cathepsin O (CTSO) |
Yeast In Vivo Biotinylation in E.coli |
CSB-EP006203HU | Recombinant Human Cathepsin O (CTSO) |
E.coli |
CSB-BP006203HU | Recombinant Human Cathepsin O (CTSO) |
Baculovirus |
CSB-MP006203HU | Recombinant Human Cathepsin O (CTSO) |
Mammalian cell |
CTSO Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP006203MO CSB-EP006203MO CSB-BP006203MO CSB-MP006203MO CSB-EP006203MO-B |
Recombinant Mouse Cathepsin O (Ctso) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSO, a gene initially cloned from a breast cancer cell library [1], was found to be mapped to chromosome 4q31-q32, which is a unique localization for cysteine proteases [2]. The gene coding enzyme CTSO is a cysteine endopeptidase that is broadly distributed in tissues, but with the highest expression in the human ovary, kidney, liver, and placenta [2]. The ubiquitous distribution of CTSO is consistent with its role in the involvement in normal cellular protein degradation and turnover [1]. CTSO is synthesized as a 329-amino acid pre-pro-enzyme with more than 50% identity to both human cathepsin S and cathepsin L and 94% identity to a rabbit cDNA, termed OC2, recently isolated from osteoclasts [3]. The sequence contains a signal peptide and a pro-domain presumably involved in maintaining enzyme latency. Removal of the pro-domain activates CTSO in the acidic lysosomal compartment. Preliminary investigations of the enzymatic activity of CTSO expressed in E. coli revealed that CTSO can cleave the substrates Z-Phe-Arg-MCA and Z-Arg-Arg-MCA although at lower rates than cathepsin B. When transfected into COS-7 cells, CTSO displayed potent endoprotease activity against fibrinogen at acid pH [3]. This proteolytic enzyme CTSO is involved in cellular protein degradation and turnover.
[1] Velasco G, Ferrando AA, et al. Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues [J]. J Biol Chem. 1994 Oct 28; 269(43):27136-42.
[2] Santamaría I, Pendás AM, et al. Genomic structure and chromosomal localization of the human cathepsin O gene (CTSO) [J]. Genomics. 1998 Oct 15; 53(2):231-4.
[3] G P Shi, H A Chapman, et al. Molecular Cloning of Human Cathepsin O, a Novel Endoproteinase and Homologue of Rabbit OC2 [J]. FEBS Lett, 1995, 357 (2), 129-34.