CTSO

The following CTSO reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CTSO Antibodies

CTSO Antibodies for Homo sapiens (Human)

CTSO Proteins

CTSO Proteins for Homo sapiens (Human)

CTSO Proteins for Mus musculus (Mouse)

CTSO Background

CTSO, a gene initially cloned from a breast cancer cell library [1], was found to be mapped to chromosome 4q31-q32, which is a unique localization for cysteine proteases [2]. The gene coding enzyme CTSO is a cysteine endopeptidase that is broadly distributed in tissues, but with the highest expression in the human ovary, kidney, liver, and placenta [2]. The ubiquitous distribution of CTSO is consistent with its role in the involvement in normal cellular protein degradation and turnover [1]. CTSO is synthesized as a 329-amino acid pre-pro-enzyme with more than 50% identity to both human cathepsin S and cathepsin L and 94% identity to a rabbit cDNA, termed OC2, recently isolated from osteoclasts [3]. The sequence contains a signal peptide and a pro-domain presumably involved in maintaining enzyme latency. Removal of the pro-domain activates CTSO in the acidic lysosomal compartment. Preliminary investigations of the enzymatic activity of CTSO expressed in E. coli revealed that CTSO can cleave the substrates Z-Phe-Arg-MCA and Z-Arg-Arg-MCA although at lower rates than cathepsin B. When transfected into COS-7 cells, CTSO displayed potent endoprotease activity against fibrinogen at acid pH [3]. This proteolytic enzyme CTSO is involved in cellular protein degradation and turnover.

[1] Velasco G, Ferrando AA, et al. Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues [J]. J Biol Chem. 1994 Oct 28; 269(43):27136-42.
[2] Santamaría I, Pendás AM, et al. Genomic structure and chromosomal localization of the human cathepsin O gene (CTSO) [J]. Genomics. 1998 Oct 15; 53(2):231-4.
[3] G P Shi, H A Chapman, et al. Molecular Cloning of Human Cathepsin O, a Novel Endoproteinase and Homologue of Rabbit OC2 [J]. FEBS Lett, 1995, 357 (2), 129-34.

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