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| Code | CSB-E04725h |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The product CSB-E04725h is a ready-to-use microwave, strip plate ELISA kit designed to quantitatively analyze the TGFB1 in different biological samples, including serum, plasma, and cell culture supernates. This TGFB1 ELISA kit specifically recognizes the human TGFB1 in the sample. It adopts the Sandwich ELISA technique in conjunction with an enzyme colorimetric reaction to indicate the presence of human TGFB1 and quantitatively measures the level of TGFB1 in the samples. The intensity of the colored product is positively related to the amount of human TGFB1 in the sample. Human TGFB1 is the most abundant TGFB isoform and was cloned from the human placenta. It is a pleiotropic growth factor that exerts multiple effects on inflammation, angiogenesis, fibrosis, tissue repair, and tumor progression. TGFB1 also exhibits various anti-inflammatory activities and contributes to immune homeostasis and inhibition of autoimmunity. TGFB1 acting as a very potent stimulator of chemotaxis stimulates monocyte, lymphocyte, neutrophil, and fibroblast migration. Dysregulated TGFB1 signaling has been associated with cellular proliferation, cancer development, and metastasis. TGFB1 upregulation mediated promotion of the epithelial-mesenchymal transition (EMT) enhances invasiveness, metastases development, angiogenesis, and immune suppression. |
| Target Name | transforming growth factor, beta 1 |
| Alternative Names | Cartilage-inducing factor ELISA Kit; CED ELISA Kit; Differentiation inhibiting factor ELISA Kit; DPD1 ELISA Kit; LAP ELISA Kit; Latency-associated peptide ELISA Kit; Prepro transforming growth factor beta 1 ELISA Kit; TGF beta 1 ELISA Kit; TGF beta ELISA Kit; TGF beta 1 protein ELISA Kit; TGF-beta 1 protein ELISA Kit; TGF-beta-1 ELISA Kit; TGF-beta-5 ELISA Kit; TGF-beta1 ELISA Kit; TGFB ELISA Kit; Tgfb-1 ELISA Kit; tgfb1 ELISA Kit; TGFB1_HUMAN ELISA Kit; TGFbeta ELISA Kit; TGFbeta1 ELISA Kit; Transforming Growth Factor b1 ELISA Kit; Transforming Growth Factor beta 1 ELISA Kit; Transforming growth factor beta 1a ELISA Kit; transforming growth factor beta-1 ELISA Kit; transforming growth factor, beta 1 ELISA Kit; Transforming Growth Factor-脽1 ELISA Kit |
| Abbreviation | TGFB1 |
| Uniprot No. | P01137 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 0.78 ng/mL-50 ng/mL |
| Sensitivity | 0.747 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision |
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays):CV%<10% Three samples of known concentration were tested in twenty assays to assess.
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| Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
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| Gene References into Functions |
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| Involvement in disease | Camurati-Engelmann disease (CAEND) |
| Subcellular Location | [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted. |
| Protein Families | TGF-beta family |
| Tissue Specificity | Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. |
| Database Links |
HGNC: 11766 OMIM: 131300 KEGG: hsa:7040 STRING: 9606.ENSP00000221930 UniGene: Hs.645227 |
