Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Transcription
Species
Escherichia coli (strain K12)
Expression Region
1-330aa
Target Protein Sequence
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-330 constitute the expression domain of recombinant Escherichia coli (strain K12) rpoS. The calculated molecular weight for this rpoS protein is 42.1 kDa. Expression of this rpoS protein is conducted in e.coli. The rpoS gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant rpoS protein during the following stages.
The Escherichia coli RNA polymerase sigma factor RpoS is a key regulator of gene expression involved in the bacterial stress response. RpoS plays a crucial role in the transition from exponential growth to stationary phase, enabling E. coli to adapt to various environmental stresses. It controls the expression of genes related to survival mechanisms, such as oxidative stress resistance, nutrient limitation, and the general stress response. RpoS is essential for bacterial virulence and persistence in different environments. Its activity is tightly regulated at the transcriptional and post-translational levels, allowing E. coli to modulate its gene expression profile in response to changing conditions. Understanding the regulatory mechanisms of RpoS is important for unraveling bacterial adaptation strategies and developing strategies to control bacterial infections.
