Recombinant Human DNA polymerase iota (POLI)

1. Findings indicate that DNA polymerase iota (poli) plays a positive role in lung cancer progression via promoting the migration and invasion of lung cancer cells. PMID: 30060029
2. Findings indicate the function of tumor suppressor protein p53 (p53) and DNA polymerase iota (POLiota) in the DNA damage response to endogenous or exogenous replication stress. PMID: 27407148
3. DNA polymerase iota increased the expression of MMP-2/9 to promote invasion and metastasis of esophageal squamous cell carcinoma. PMID: 27057634
4. The predominant isoform of DNA poliota in human cells is the shorter 715 amino acid protein and that if, or when, expressed, the longer 740 amino acid isoform has identical properties to the considerably more abundant shorter isoform. PMID: 28865289
5. Kinetic and Structural Impact of Metal Ions and Genetic Variations on Human DNA Polymerase iota. PMID: 27555320
6. Mass spectrometry analysis of monoubiquitinated poliota revealed that it is ubiquitinated at over 27 unique sites. Many of these sites are localized in different functional domains of the protein. PMID: 26370087
7. Germline genetic variations in human POLI gene may either hinder or promote the translesion synthesis (TLS) capability of pol iota with various DNA lesions in vitro, emphasizing the potential translational importance of these pol iota genetic variations, e.g., individual differences in TLS, mutation, and cancer susceptibility to genotoxic carcinogens. PMID: 25162224
8. a single residue in pol iota is able to discriminate between NTPs and dNTPs during DNA synthesis. PMID: 24532793
9. Dysregulation of pol iota by JNK/c-Jun is involved in carcinogenesis and offer a novel understanding of the role of pol iota or c-Jun in mutagenesis. PMID: 23922701
10. Human Pol iota and yeast Pol zeta complex could function efficiently in the insertion and extension steps, respectively, of ranslesion synthesis and human Pol kappa and Pol eta could also extend past these lesions, albeit much less efficiently. PMID: 23965998
11. POLI and MC4R nearby single nucleotide polymorphisms in human chromosome 18 are associated with a moderate risk of type 2 diabetes. PMID: 23727064
12. Results show that the physical and functional interaction between pols eta and iota occurs between ubiquitinated forms of either polymerase via their respective ubiquitin-binding domains. PMID: 23248005
13. this review briefly discusses the main structural features and possible functional mechanisms of the active center of Pol iota. [review] PMID: 22817454
14. in mammalian cells, both polymerases kappa and iota are necessary for the error-free bypass of N(2)-CEdG and N(2)-CMdG. PMID: 21454642
15. structural mechanism of high-fidelity 8-oxo-G replication by a human DNA polymerase PMID: 21300901
16. Structural basis for proficient incorporation of dTTP opposite O6-methylguanine by human DNA polymerase iota. PMID: 20961860
17. In the presence of Mn2+, the Pol iota activity in carcinoma was 2.5-fold higher than the control cells. PMID: 20673215
18. The solution structures of the C-terminal UBM of human pol iota and its complex with ubiquitin are reported. PMID: 20159559
19. Variants in POLI is associated with predisposition for TMPRSS2-ERG fusion in prostate cancer. PMID: 19861517
20. DNA polymerase iota's versatility implies that this enzyme can adjust to local structural conditions in order to bypass bulky lesions by strategies that utilize Watson-Crick, Hoogsteen and perhaps other base pairing possibilities. PMID: 19767609
21. By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota PMID: 12410315
22. DNA polymerase iota associates with the replication machinery & accumulates at stalled replication forks following DNA-damaging treatment. Its the C-terminal 224 AAs are sufficient for both the interaction with poleta & accumulation in replication foci. PMID: 12426396
23. Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by DNA polymerase iota. PMID: 12466554
24. pol iota can complement the in vitro single-nucleotide BER deficiency of a DNA polymerase beta null cell extract PMID: 12777390
25. propose that the incipient base pair is accommodated differently in the active site of Poliota dependent upon the template base and that when T is the templating base, Poliota accommodates the wobble base pair better than the Watson-Crick base pair PMID: 14701763
26. crystal structure bound to a template primer and an incoming nucleotide; structure reveals a polymerase that is 'specialized' for Hoogsteen base-pairing, whereby the templating base is driven to the syn conformation PMID: 15254543
27. Data strongly suggest that pol iota may be involved in the generation of both increased spontaneous and translesion mutations during DNA replication in breast cancer cells, thereby contributing to the accumulation of genetic damage. PMID: 15313897
28. Data show that an interaction between human DNA polymerase iota (poliota) and the proliferating cell nuclear antigen (PCNA) stimulates the processivity of poliota in a template-dependent manner in vitro. PMID: 15342632
29. Poliota interacts with PCNA via only one of its conserved PCNA binding motifs, regardless of whether PCNA is bound to DNA or not. PMID: 15657443
30. DNA polymerase iota promotes replication through a ring-closed minor-groove adduct that adopts a syn conformation in DNA PMID: 16166652
31. The sequential action of Poliota and Polkappa promotes efficient and error-free synthesis through the HNE-dG adducts. PMID: 16354708
32. identification of two previously unknown ubiquitin-binding domains in the Y-family translesion synthesis polymerases that enable them to interact with monoubiquitinated targets and undergo monoubiquitination in vivo PMID: 16357261
33. results show that Pol iota has no significant role in UV lesion bypass and mutagenesis in vivo and provides some initial data suggesting that this polymerase may be involved in replication of extrachromosomal DNA PMID: 16472831
34. polymerization by pol iota is severely inhibited by a bulky group at G N2 despite an advantageous mode of Hoogsteen base pairing PMID: 16527824
35. DNA polymerases iota and eta interact noncovalently with free polyUb chains, as well as mono-ubiquitinated proliferating cell nuclear antigen (Ub-PCNA). PMID: 16763556
36. For proficient T incorporation opposite template A, only the N7 hydrogen bonding is required, but for proficient C incorporation opposite template G, hydrogen bonding at both the N7 and O(6) is an imperative. PMID: 16914729
37. These results suggest that HIF-1-mediated pol iota gene expression may be involved in the generation of translesion mutations during DNA replication after hypoxia followed by reoxygenation. PMID: 17056006
38. the cation utilized by pol iota in vivo may actually be Mn(2+) rather than Mg(2+), as tacitly assumed PMID: 17609217
39. Data suggest that DNA polymerases eta and iota transiently probe DNA/chromatin; when DNA is exposed at replication forks, the polymerase residence times increase, and this is further facilitated by the ubiquitination of PCNA. PMID: 18799611
40. These data reveal a novel role of human DNA pol iota in protecting cells from oxidative damage. PMID: 18923427
41. Lesion bypass of N2-ethylguanine by human DNA polymerase iota. PMID: 18984581
42. enzyme plays important functions in protecting humans from the deleterious consequences of exposure to both oxidative- and ultraviolet light-induced DNA damage PMID: 19162565
43. DNA synthesis across an absaic lesion by human POLI is reported. PMID: 19368886
44. Structural and domain-swapping experiments indicate that the finger domain is responsible for DNA polymerase iota's high error rates on pyrimidines and determines the incorporation specificity. PMID: 19440206
45. Replication across template T/U by human DNA POLI is reported. PMID: 19604477

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HGNC: 9182

OMIM: 605252

KEGG: hsa:11201

STRING: 9606.ENSP00000462664

UniGene: Hs.438533