Recombinant Human Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2)

Code CSB-YP018005HU
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Source Yeast
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Code CSB-EP018005HU
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Source E.coli
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Code CSB-EP018005HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018005HU
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Source Baculovirus
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Code CSB-MP018005HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PIK3R2
Uniprot No. O00459
Alternative Names p85; p85 beta; P85B; P85B_HUMAN; Phosphatidylinositol 3 kinase; Phosphatidylinositol 3 kinase regulatory beta subunit ; Phosphatidylinositol 3 kinase regulatory subunit beta; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2; Phosphatidylinositol 3 kinase; regulatory subunit; polypeptide 2 (p85 beta); Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphoinositide 3 kinase regulatory subunit 2 (beta); Phosphoinositide 3 kinase regulatory subunit 2; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta); Phosphoinositide 3 kinase regulatory subunit polypeptide 2; Phosphoinositide 3 kinase; regulatory subunit 2 (beta); Phosphoinositide 3 kinase; regulatory subunit 2 (p85 beta); PI3 kinase p85 beta subunit ; PI3 kinase p85 subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K; PI3K regulatory subunit beta; PIK3R 2; PIK3R2; PtdIns 3 kinase p85 beta; PtdIns-3-kinase regulatory subunit beta; PtdIns-3-kinase regulatory subunit p85-beta
Species Homo sapiens (Human)
Expression Region 1-728
Target Protein Sequence MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGGERC PQSVGWMPGL NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP RDGAPEPGLT LPDLPEQFSP PDVAPPLLVK LVEAIERTGL DSESHYRPEL PAPRTDWSLS DVDQWDTAAL ADGIKSFLLA LPAPLVTPEA SAEARRALRE AAGPVGPALE PPTLPLHRAL TLRFLLQHLG RVASRAPALG PAVRALGATF GPLLLRAPPP PSSPPPGGAP DGSEPSPDFP ALLVEKLLQE HLEEQEVAPP ALPPKPPKAK PASTVLANGG SPPSLQDAEW YWGDISREEV NEKLRDTPDG TFLVRDASSK IQGEYTLTLR KGGNNKLIKV FHRDGHYGFS EPLTFCSVVD LINHYRHESL AQYNAKLDTR LLYPVSKYQQ DQIVKEDSVE AVGAQLKVYH QQYQDKSREY DQLYEEYTRT SQELQMKRTA IEAFNETIKI FEEQGQTQEK CSKEYLERFR REGNEKEMQR ILLNSERLKS RIAEIHESRT KLEQQLRAQA SDNREIDKRM NSLKPDLMQL RKIRDQYLVW LTQKGARQKK INEWLGIKNE TEDQYALMED EDDLPHHEER TWYVGKINRT QAEEMLSGKR DGTFLIRESS QRGCYACSVV VDGDTKHCVI YRTATGFGFA EPYNLYGSLK ELVLHYQHAS LVQHNDALTV TLAHPVRAPG PGPPPAAR
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy. Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Gene References into Functions
  1. Data indicate that miR-126 overexpression in RASFs inhibits PIK3R2 expression and promotes proliferation while inhibiting apoptosis. PMID: 27729613
  2. this study shows that targeted depletion of PIK3R2 induces regression of lung squamous cell carcinoma PMID: 27835880
  3. Data show that phosphoinositol-3 kinase regulatory subunit 2 (PIK3R2) mRNA is a direct target of miR-126-3p. PMID: 27191494
  4. Data indicate that miR-126 expression negatively correlates with p85beta in CLL patients and that miR-126 can effectively target p85beta in a cell-line system. PMID: 28299881
  5. Mosaic mutations in PIK3CA or PIK3R2 which activate class 1A PI3K cause severe non-ketotic hypoglycaemia in a subset of patients, with the metabolic phenotype presumably related to the extent of mosaicism within the liver PMID: 28566443
  6. PIK3R2 D557H mutation causes polymicrogyria, corpus callosum hyperplasia and focal cortical dysplasia. PMID: 26860062
  7. miR-3151 silencing by DNA methylation protected chronic lymphocytic leukemia cells from apoptosis by over-expression of its direct targets MADD and PIK3R2, constitutive activation of MEK/ERK and PI3K/AKT signaling , and over-expression of MCL1. PMID: 26517243
  8. pik3r2 was confirmed to be a direct target of miR126 in prostate cancer. PMID: 26677064
  9. indicated that miR-126 expression was negatively correlated with PIK3R2 mRNA expression PMID: 26723864
  10. The miR-126 was found to act as proliferation suppressor targeting PIK3R2 gene and reducing p85beta (a regulatory subunit of PI3K kinase) protein translation and lower AKT kinase activity. PMID: 26384552
  11. Constitutional and mosaic mutations in the PIK3R2 gene are associated with developmental brain disorders ranging from Bilateral perisylvian polymicrogyria with a normal head size to the megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome. PMID: 26520804
  12. The authors show that this potentiation involves reorganization of the natural CrkL-p85beta complex into a novel trimeric complex where influenza A virus NS1 serves as a bridging factor. PMID: 26099693
  13. the metastasis and angiogenesis functions of miR-126-3p were mediated by LRP6 and PIK3R2. PMID: 25240815
  14. Study indicates that miR-126 is a tumor suppressor that inhibits gastric cancer cells proliferation by targeting PI3KR2, Crk and PLK2. PMID: 24969300
  15. FBXL2 mediates the ubiquitylation and degradation of p85beta on cell membranes. PMID: 23604317
  16. miR-126-mediated phosphoinositide-3-kinase regulation, not only fine-tunes VEGF-signaling, but it strongly enhances the activities of Ang-1 on vessel stabilization and maturation. PMID: 22867989
  17. p85beta phosphoinositide 3-kinase subunit regulates tumor progression PMID: 22733740
  18. recombinant production, crystallization and X-ray structure determination at 2.0 A resolution of the SH3 domain of human p85beta is described PMID: 22102226
  19. miR-126 could target both VEGFA and PIK3R2, and its expression was decreased in human breast cancer, implying that miR-126 may play a role in tumor genesis and growth by regulating the VEGF/PI3K/AKT signaling pathway PMID: 21249429
  20. The crystal structure of human p85beta iSH2 determined to 3.3A resolution is reported. PMID: 21139197
  21. Macropinocytosis is regulated by interactions between Abi1 pY213 and the C-terminal SH2 domain of p85-thereby linking Abl kinase signaling to p85-dependent regulation of macropinocytosis. PMID: 20598684
  22. Overexpression of SH3 domain of p85beta inhibits influenza A virus replication. PMID: 20653952
  23. the PTEN phosphatase is active against the PI3K p85beta subunit and dephosphorylates a protein involved in insulin signaling where known downstream consequences are increased cell migration, motility, and invasion. PMID: 20515662
  24. oncogenic p85 mutations lead to a loss of a C2-iSH2 domain contact that is required for inhibition of p110alpha by p85. PMID: 19915146
  25. NS5A mediates activation of beta-catenin in a phosphoinositide-3 kinase-dependent fashion. PMID: 19846673
  26. Mutant viruses carrying NS1 with mutations in SH3 binding motif 1 failed to interact with p85ss and induce the subsequent activation of PI3K/Akt pathway. PMID: 17881440
  27. in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration PMID: 18420583

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Involvement in disease Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 (MPPH1)
Protein Families PI3K p85 subunit family
Database Links

HGNC: 8980

OMIM: 603157

KEGG: hsa:5296

STRING: 9606.ENSP00000471914

UniGene: Hs.371344

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