Recombinant Mouse DNA polymerase iota (Poli)

Code	CSB-YP750922MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP750922MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP750922MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP750922MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP750922MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Poli

Uniprot No.

Q6R3M4

Alternative Names

Poli; Rad30b; DNA polymerase iota; EC 2.7.7.7; Rad30 homolog B

Species

Mus musculus (Mouse)

Expression Region

1-717

Target Protein Sequence

MEPLHAGAAG SSRAVCSQGP PTQISSSRVI VHVDLDCFYA QVEMISNPEL KDRPLGVQQK YLVVTCNYEA RKLGVRKLMN VRDAKEKCPQ LVLVNGEDLS RYREMSYKVT ELLEEFSPAV ERLGFDENFV DLTEMVEKRL QQLPSEEVPS VTVFGHVYNN QSVNLHNIMH RRLVVGSQIA AEMREAMYNQ LGLTGCAGVA PNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKRL EVLGINSVHD LQTFPIKTLE KELGIAIAQR IQQLSFGEDK SPVTPSGPPQ SFSEEDTFKK CSSEVEAKAK IEELLSSLLT RVCQDGRKPH TVRLVIRRYS DKHCNRESRQ CPIPSHVIQK LGTGNHDSMP PLIDILMKLF RNMVNVKMPF HLTLMSVCFC NLKALSSAKK GPMDCYLTSL STPAYTDKRA FKVKDTHTED SHKEKEANWD CLPSRRIEST GTGESPLDAT CFPKEKDTSD LPLQALPEGV DQEVFKQLPA DIQEEILYGK SRENLKGKGS LSCPLHASRG VLSFFSTKQM QASRLSPRDT ALPSKRVSAA SPCEPGTSGL SPGSTSHPSC GKDCSYYIDS QLKDEQTSQG PTESQGCQFS STNPAVSGFH SFPNLQTEQL FSTHRTVDSH KQTATASHQG LESHQGLESR ELDSAEEKLP FPPDIDPQVF YELPEEVQKE LMAEWERAGA ARPSAHR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Target Background

Function

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Gene References into Functions

Pol iota occasionally accesses the replication fork to generate a first mutation, and Pol zeta extends the mismatch with a second mutation. PMID: 27455952
Mouse DNA polymerase iota lacking exon 2 (42 amino acids) is catalytically inactive in vitro. PMID: 28077247
Exon 2 deletion abrogates both the DNA polymerase and dRP lyase activities of Pol iota in the presence of either Mg(2+) or Mn(2+) ions. Thus, 129-derived strains of mice express catalytically inactive alternatively spliced Pol iota variant. PMID: 28077248
PolH contributes to accurate translesion synthesis (TLS) past both T- & C-containing dimers. PolI is involved in error-prone TLS past cytosine-containing dimers when Poleta is inactivated. PMID: 25733082
Poliota causes the Par2 effect and inhibits tumorigenesis and mutagenesis PMID: 23955086
in mammalian cells, both polymerases kappa and iota are necessary for the error-free bypass of N(2)-CEdG and N(2)-CMdG. PMID: 21454642
Upon DNA damage, the UBDs (UBM domains) of polymerase iota (Pol iota) interact with ubiquitinated proliferating cell nuclear antigen to regulate the interchange between processive DNA polymerases and translesional synthesis PMID: 20929865
In the presence of Mg2+, the enzyme was active only in testicles and brain, whereas in the presence of Mn2+ the activity was found in all organs. PMID: 20673215
Data suggest that either DNA polymerase iota does not participate in hypermutation, or its role is nonessential and can be readily assumed by another low-fidelity polymerase. PMID: 12925679
Nucleotide polymorphisms in DNA polymerase iota is associated with lung tumorigenesis in mouse and humans PMID: 15026325
Pol kappa and Pol iota double-deficient mice had the normal somatic hypermutation frequency PMID: 15860226
Pol iota gene may participate in error-free repair of damaged DNA and prevention of lung tumor development PMID: 15904465
identification of two previously unknown ubiquitin-binding domains in the Y-family translesion synthesis polymerases that enable them to interact with monoubiquitinated targets and undergo monoubiquitination in vivo PMID: 16357261
suggest the involvement of the Pol eta and Pol iota proteins in UV-induced skin carcinogenesis PMID: 17015482
Although pol iota deficiency alone had no effect, UV-induced skin tumors in pol eta-deficient mice developed 4 weeks earlier in mice concomitantly deficient in pol iota PMID: 17114294