Code | CSB-RA187762A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
MAPK1, also called ERK2, is a serine/threonine-protein kinase belonging to the mitogen-activated protein kinase (MAPK) family and is widely found in eukaryotic cells. It plays a central role in signal transduction from surface receptors to the nucleus through the classical Ras-Raf-MEK-ERK1/2 pathway. Activated ERK2 phosphorylates substrates in the cytoplasm or nucleus, and thus induce expression or activation of specific proteins, resulting in regulation of cell growth, development, proliferation, differentiation, apoptosis, and other processes.
The first step in the preparation of recombinant MAPK1 antibody is to obtain the MAPK1 antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant MAPK1 antibody was obtained using Affinity-chromatography. This recombinant MAPK1 antibody has been validated for the detection of MAPK1 protein from Human, Mouse, Rat in the ELISA, WB, IHC, IF.There are currently no reviews for this product.