CD4

CD4, recognized as a surface antigen, is a glycoprotein found on the membranes of a specific subset of T cells, predominantly Helper T cells. These cells are integral to the human immune system. CD4 enables T cells to identify and react to antigens by interacting with major histocompatibility complex (MHC) class II molecules, thereby triggering T cell activation and initiating an immune response. Moreover, CD4 serves as the primary receptor for HIV to enter human cells, which exhibits significant importance in the diagnosis and treatment of HIV/AIDS. The CD4+ T cells are crucial regulators within the immune system, tasked with the elimination and control of various infections and the defense against invading pathogens, constituting a vital component of human immune defense mechanisms.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF004935HU1 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CD4.

CD4 Recombinant Monoclonal Antibody (CSB-RA004935A0HU)

Validated Data

Western Blot
Positive WB detected in Jurkat whole cell lysate
All lanes CD4 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa

IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Human cluster of differentiation 4,CD4 ELISA Kit (CSB-E08956h)

Validated Data

Code: CSB-E08956h

Size: 96T,5×96T,10×96T

Sensitivity: 0.156 ng/mL

Detection Range: 0.625 ng/mL-40 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following CD4 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CD4 Antibodies

CD4 Proteins

CD4 ELISA Kits

CD4 Antibodies

CD4 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA004935GA01HU	CD4 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA004935ESR2HU	CD4 Antibody	Human	ELISA, IHC
CSB-PA001453	CD4 Antibody	Human,Mouse	WB, IHC, IF, ELISA
CSB-PA070257	Phospho-CD4 (S433) Antibody	Human,Mouse	IHC, IF, ELISA
CSB-RA004935A0HU	CD4 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC

CD4 Antibodies for H, M, R

Code	Product Name	Species Reactivity	Application
CSB-MA000228	CD4 Monoclonal Antibody	Human,Mouse,Rat	ELISA,IHC

CD4 Proteins

CD4 Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-YP004935SH CSB-EP004935SH CSB-BP004935SH CSB-MP004935SH CSB-EP004935SH-B	Recombinant Sheep T-cell surface glycoprotein CD4 (CD4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP004935HU1 CSB-EP004935HU1 CSB-BP004935HU1 CSB-EP004935HU1-B	Recombinant Human T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli
CSB-CF004935HU	Recombinant Human T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system

CD4 Proteins for Macaca nemestrina (Pig-tailed macaque)

Code	Product Name	Source
CSB-YP600482MOX CSB-EP600482MOX CSB-BP600482MOX CSB-MP600482MOX CSB-EP600482MOX-B	Recombinant Macaca nemestrina T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys)

Code	Product Name	Source
CSB-YP600852DRI CSB-EP600852DRI CSB-BP600852DRI CSB-MP600852DRI CSB-EP600852DRI-B	Recombinant Cercocebus atys T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Saimiri sciureus (Common squirrel monkey)

Code	Product Name	Source
CSB-YP639036SWD CSB-EP639036SWD CSB-BP639036SWD CSB-MP639036SWD CSB-EP639036SWD-B	Recombinant Saimiri sciureus T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Delphinapterus leucas (Beluga whale)

Code	Product Name	Source
CSB-YP894546DJB CSB-EP894546DJB CSB-BP894546DJB CSB-MP894546DJB CSB-EP894546DJB-B	Recombinant Delphinapterus leucas T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-CF004935RA	Recombinant Rat T-cell surface glycoprotein CD4 (Cd4)	in vitro E.coli expression system
CSB-YP004935RA1 CSB-EP004935RA1 CSB-BP004935RA1 CSB-MP004935RA1 CSB-EP004935RA1-B	Recombinant Rat T-cell surface glycoprotein CD4 (Cd4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-CF004935MO	Recombinant Mouse T-cell surface glycoprotein CD4 (Cd4)	in vitro E.coli expression system
CSB-YP004935MO1 CSB-EP004935MO1 CSB-BP004935MO1 CSB-MP004935MO1 CSB-EP004935MO1-B	Recombinant Mouse T-cell surface glycoprotein CD4 (Cd4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Macaca mulatta (Rhesus macaque)

Code	Product Name	Source
CSB-CF004935MOW	Recombinant Macaca mulatta (Rhesus macaque) T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935MOW1 CSB-EP004935MOW1 CSB-BP004935MOW1 CSB-MP004935MOW1 CSB-EP004935MOW1-B	Recombinant Macaca mulatta (Rhesus macaque) T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-CF004935MOV	Recombinant Macaca fascicularis T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935MOV1 CSB-EP004935MOV1 CSB-BP004935MOV1 CSB-MP004935MOV1 CSB-EP004935MOV1-B	Recombinant Macaca fascicularis T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Macaca fuscata fuscata (Japanese macaque)

Code	Product Name	Source
CSB-CF004935MMX	Recombinant Macaca fuscata fuscata T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935MMX1 CSB-EP004935MMX1 CSB-BP004935MMX1 CSB-MP004935MMX1 CSB-EP004935MMX1-B	Recombinant Macaca fuscata fuscata T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-CF004935EQV	Recombinant Pan troglodytes T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935EQV1 CSB-EP004935EQV1 CSB-BP004935EQV1 CSB-MP004935EQV1 CSB-EP004935EQV1-B	Recombinant Pan troglodytes T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Source
CSB-CF004935RB	Recombinant Rabbit T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935RB1 CSB-EP004935RB1 CSB-BP004935RB1 CSB-MP004935RB1 CSB-EP004935RB1-B	Recombinant Rabbit T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD4 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Source
CSB-CF004935DO	Recombinant Dog T-cell surface glycoprotein CD4 (CD4)	in vitro E.coli expression system
CSB-YP004935DO1 CSB-EP004935DO1 CSB-BP004935DO1 CSB-MP004935DO1 CSB-EP004935DO1-B	Recombinant Dog T-cell surface glycoprotein CD4 (CD4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli