Rat cluster of differentiation 4,CD4 ELISA Kit

Code CSB-E12928r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat CD4 ELISA Kit was designed for the quantitative measurement of Rat CD4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 U/mL-50 U/mL and the sensitivity is 0.195 U/mL.

Target Name CD4 molecule
Alternative Names Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4
Abbreviation CD4
Uniprot No. P05540
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 U/mL-50 U/mL
Sensitivity 0.195 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 97    
Range % 87-102    
1:2 Average % 101    
Range % 94-105    
1:4 Average % 99    
Range % 88-103    
1:8 Average % 105    
Range % 98-109    
Recovery
The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 85-98    
EDTA plasma (n=4) 98 89-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
U/ml OD1 OD2 Average Corrected    
50 2.656 2.587 2.622 2.508    
25 2.171 2.062 2.117 2.003    
12.5 1.438 1.388 1.413 1.299    
6.25 0.720 0.715 0.718 0.604    
3.12 0.412 0.424 0.418 0.304    
1.56 0.261 0.252 0.257 0.143    
0.78 0.176 0.183 0.180 0.066    
0 0.112 0.115 0.114      
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Gene References into Functions
  1. Adoptive transfusion of CD4+CD25+ Regulatory T Cells into Experimental Autoimmune Neuritis model rats has significant therapeutic effects. PMID: 29772575
  2. Increased C4d deposition may be amenable to identify early myocardial infarction after cardiac arrest. PMID: 26467126
  3. Data show several homologies across human, rat and mouse in the kinetic of loss of several LAgs such as CD5, CD4 and CD28. PMID: 21745657
  4. testicular macrophages expressed the markers CD45 and MAC387 and most also expressed CD4. PMID: 11994538
  5. C4d deposition and cellular infiltrates as markers of acute rejection in rat models of orthotopic lung transplantation. PMID: 18622289
  6. In this EAE model, perivascular cells, microglia and macrophages showed different dynamics; microglial cells expressed CD4 during the recovery phase, suggesting microglial CD4 expression in the resolution of the immune response. PMID: 19246105
  7. Clear trend toward worse graft survival in patients with focal C4d after kidney transplantaation. PMID: 19344468
  8. Capillary C4d deposition is a marker of antibody-mediated rejection in kidney transplantation and can be detected by various staining methods. PMID: 19376373

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Subcellular Location Cell membrane; Single-pass type I membrane protein.
Database Links

KEGG: rno:24932

STRING: 10116.ENSRNOP00000021915

UniGene: Rn.10748

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