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The CD4 recombinant monoclonal antibody is a highly specific antibody against the human CD4 protein. This CD4 antibody was produced in vitro by transfecting human CD4 monoclonal antibody gene- vector clones into the cell line, followed by affinity-chromatography purification from the tissue culture supernatant (TCS). The mice were immunized with a human CD4 synthetic peptide to produce the human CD4 monoclonal antibody. This CD4 antibody has the same isotype as rabbit IgG. It can be applied in ELISA, WB, and IHC.
T cells, natural killer (NK) cells, monocytes, and macrophages all express the CD4 coreceptor. CD4 regulates differentiation, migration, and cytokine expression in macrophages and NK cells. It plays a role in thymic development and antigen recognition in the periphery in T lymphocytes.
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