Alkaline phosphatase (abbreviated as ALP or AKP, AP) is a monophospholipid hydrolase widely distributed in human liver, bone, intestine, kidney and placenta. This enzyme catalyzes the removal of the 5' phosphate from nucleic acid molecules, thereby converting the 5'-P terminus of a DNA or RNA fragment to a 5'-OH terminus.
AKP is a membrane-bound metalloglycoprotein composed of two subunits. As a homodimeric enzyme, AKP includes four isoenzymes, involving tissue nonspecific AP (TNAP), intestinal AP (IAP), placental AP (PLAP), and PLAP-like.
TNAP is mainly expressed in liver, kidney and bone tissue, namely liver/bone/kidney type AP. Among them, bone-type AP is an extracellular enzyme of osteoblasts, which can be released into the blood circulation. Its main physiological function is to hydrolyze phosphate esters in the process of osteogenesis to provide the necessary phosphoric acid for the deposition of hydroxyapatite. At the same time, it hydrolyzes pyrophosphate to relieve its inhibitory effect on the formation of bone salt, which is beneficial to the osteogenesis process.
IAP, PLAP and PLAP-like are tissue-specific AKPs. IAP is a brush border protein that can hydrolyze monophosphates and can be further divided into fetal IAP and adult IAP. This protein is secreted by intestinal mucosal epithelial microvilli, distal end of renal proximal convoluted tubule and uterus. Fetal IAP, also known as Kasahara isoenzyme, is used as a tumor marker and appears in the serum of patients with tumors in liver cancer and other organs. PLAP is mainly secreted by the microvilli of placental syncytiotrophoblast cells after 8 weeks of gestation, and a small amount of PLAP is also secreted by the lungs, ovaries, sub-organs, gastrointestinal tract and other organs. The active transport and calcium and phosphorus metabolism of the fetus provide nutrients for fetal growth and development, and play an important role in the growth and development of the fetus. Dynamic observation of placental alkaline phosphatase activity can be used as an indicator of pregnancy. PLAP-like, also known as Nagao isoenzyme or GCAP, a tumor marker that appears in a variety of cancers, such as seminoma, ovarian cancer, etc.
Popular Product:
● Recombinant Rat Intestinal-type alkaline phosphatase 1(Alpi) (Active) (CSB-MP001627RA)
Validated Data
Less than 1.0 EU/ug as determined by LAL method.
Greater than 95% as determined by SDS-PAGE.
Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate (pNPP), in 1 minute at 37°C, pH10.0.The specific activity is > 10370.37 U/mg.
● Recombinant Human Intestinal-type alkaline phosphatase(ALPI) (Active) (CSB-MP001627HU)
Validated Data
Less than 1.0 EU/ug as determined by LAL method.
Greater than 95% as determined by SDS-PAGE.
Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate (pNPP), in 1 minute at 37°C, pH10.0. The specific activity is > 8836.463 U/mg.
The following ALPI reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ALPI Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA11989B0Rb | ALPI Antibody, HRP conjugated |
Human | ELISA |
CSB-PA11989A0Rb | ALPI Antibody |
Human | ELISA, WB |
CSB-PA921124 | ALPI Antibody |
Human | ELISA,IHC |
ALPI Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP001627BO CSB-EP001627BO CSB-BP001627BO CSB-MP001627BO CSB-EP001627BO-B |
Recombinant Bovine Intestinal-type alkaline phosphatase (ALPI) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ALPI Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-EP001627RA CSB-BP001627RA CSB-EP001627RA-B |
Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) |
E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-YP001627RA | Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) |
Yeast |
CSB-MP001627RA | Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active) |
Mammalian cell |
ALPI Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) |
Yeast |
CSB-BP001627HU CSB-EP001627HU-B |
Recombinant Human Intestinal-type alkaline phosphatase (ALPI) |
Baculovirus In Vivo Biotinylation in E.coli |
CSB-RP119894h | Recombinant Human Intestinal-type alkaline phosphatase (ALPI), partial |
E.coli |
CSB-YP001627HU1 CSB-EP001627HU1 CSB-BP001627HU1 CSB-MP001627HU1 CSB-EP001627HU1-B |
Recombinant Human Intestinal-type alkaline phosphatase (ALPI), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) |
E.coli |
CSB-MP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) (Active) |
Mammalian cell |
ALPI ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E09709h | Human Intestinal-type alkaline phosphatase,ALPI ELISA Kit |
serum, plasma, tissue homogenates | 0.78 ng/mL |