Mitochondria are the main energy-supplying organelles of cells, with a high degree of dynamic activity. Under normal conditions, mitochondrial fusion and fragmentation are in equilibrium. Once the balance is broken, the fragmentation of mitochondria increases, resulting in an increase in pathological apoptosis.
OMA1 is a zinc ion metalloproteinase located in the inner membrane of mitochondria. It is also a redox-dependent protein with multiple transmembrane domains and zinc finger binding motifs. It is expressed by OMA1 gene, and OMA1 protease is an important regulator of OPA1, which cleaves OPA1 under cellular stress. The dynamic balance of mitochondrial fragmentation-fusion can be controlled by regulating the hydrolysis of intimal protein OPA1. OPA1 is a member of the mitochondrial molding protein family. The protein is located in the mitochondrial intima and is an important protein regulating mitochondrial intimal fusion.
Metalloproteinase OMA1 is located on chromosome 1p32.2-p32.1 and contains 9 exons. In humans, the protein encoded by the OMA1 gene consists of 524 amino acids.
The molecular weight of human metallo-endonuclease OMA1, is 60.1 kDa, and the amino acid of 1-13 is the signal peptide.
OMA1 and m-AAA protease have overlapping activity. The human lineal homologous gene of OMA1 is MPRP-1.
Mammalian OMA1 shows an extended carboxyl terminal and a positively charged amino-terminal domain, which is considered to be important for the activation of OMA1, because the mutation of the positively charged domain impairs the activation of OMA1 without affecting its proteolytic function. OMA1 itself has also been found to be cut and inactivated under pressure, which is attributed to autocatalytic processing or YME1L1-mediated cleavage.
OMA1 is widely expressed in the body, and is highly expressed in the heart, skeletal muscle, kidney and liver.
When mitochondrial membrane potential or ATP is lost, OMA1 can degrade OPA1. This induced proteolysis is the main regulatory mechanism of proteolytic inactivation OPA1.
Under the condition of OMA1 deletion, the cleavage of OPA1 at S1 site is blocked, but the morphology of mitochondria is not greatly affected, but under pressure stimulation, mitochondria are fragmented, which requires OMA1 to cut OPA1. OMA1-deficient mice can survive but suffer from diet-induced obesity and abnormal thermogenesis, indicating that OMA1 plays an important role in OPA1 cutting in maintaining metabolic balance.
After stress, the activation of protease OMA1 will over-hydrolyze OPA1, and then promote mitochondrial breakage. If it persists, it will cause cell death and tissue degeneration in the body, and then lead to a series of diseases, such as autosomal optic nerve atrophy ((DOA)), Barthes syndrome, neurodegenerative diseases and cancer.
OMA1 is a metallo-endopeptidase, which initiates the proteolysis of OPA1 and deteriorates the structure and function of mitochondria. Researchers have demonstrated that HRI or HO stimulation can induce self-cleavage of OMA1 and change OPA1 from long to short, leading to mitochondrial breakage, cytochrome c release and apoptosis. The candidate compounds of OMA1 inhibitors were also selected: epigallocatechin gallate (EGCG) and betaine. The study also suggests the use of EGCG-induced OMA1 inhibition to treat cardiac IRI.
It is generally believed that mitochondria play an important role in tumorigenesis. Therefore, understanding the potential mechanism of mitochondria in cancer progression has potential significance for the development of new therapeutic strategies. Some studies have shown that the low expression of OMA1 is related to the low overall survival rate of breast cancer patients. The loss of OMA1 increases the migration characteristics of metastatic breast cancer cells, indicating that OMA1 plays a key role in inhibiting the metastatic ability of breast tumors. The decrease of OMA1 activity/level provides insight into the mechanisms leading to the development of breast cancer, promoting malignant progression of cancer cells and adverse clinical outcomes, which may be a possible prognostic marker and therapeutic target for the treatment of breast cancer.
Hot Products of OMA1
Recombinant Human Metalloendopeptidase OMA1, mitochondrial(OMA1) (Active) (CSB-CF856923HU)
Validated Data
Greater than 90% as determined by SDS-PAGE.
Immobilized OMA1 at 5 μg/ml can bind human BZW2, the EC50 of human BZM2 is 45.42-72.22 μg/ml.
The following OMA1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
OMA1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA016332GA01HU | OMA1 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA856923LA01HU | OMA1 Antibody |
Human | ELISA, WB, IHC, IF |
CSB-PA856923LB01HU | OMA1 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA856923LC01HU | OMA1 Antibody, FITC conjugated |
Human | |
CSB-PA856923LD01HU | OMA1 Antibody, Biotin conjugated |
Human | ELISA |
OMA1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA339648XA01SVG | OMA1 Antibody |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
OMA1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA882230XA01SXV | oma1 Antibody |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ELISA, WB (ensure identification of antigen) |
OMA1 Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA792972XA01DIL | oma1 Antibody |
Danio rerio (Zebrafish) (Brachydanio rerio) | ELISA, WB (ensure identification of antigen) |
OMA1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Source |
---|---|---|
CSB-CF882230SXV | Recombinant Schizosaccharomyces pombe Mitochondrial metalloendopeptidase OMA1 (oma1) |
in vitro E.coli expression system |
CSB-YP882230SXV1 CSB-EP882230SXV1 CSB-BP882230SXV1 CSB-MP882230SXV1 CSB-EP882230SXV1-B |
Recombinant Schizosaccharomyces pombe Mitochondrial metalloendopeptidase OMA1 (oma1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
OMA1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Source |
---|---|---|
CSB-CF339648SVG | Recombinant Saccharomyces cerevisiae Mitochondrial metalloendopeptidase OMA1 (OMA1) |
in vitro E.coli expression system |
CSB-YP339648SVG1 CSB-EP339648SVG1 CSB-BP339648SVG1 CSB-MP339648SVG1 CSB-EP339648SVG1-B |
Recombinant Saccharomyces cerevisiae Mitochondrial metalloendopeptidase OMA1 (OMA1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
OMA1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF887550MO | Recombinant Mouse Metalloendopeptidase OMA1, mitochondrial (Oma1) |
in vitro E.coli expression system |
CSB-YP887550MO1 CSB-EP887550MO1 CSB-BP887550MO1 CSB-MP887550MO1 CSB-EP887550MO1-B |
Recombinant Mouse Metalloendopeptidase OMA1, mitochondrial (Oma1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
OMA1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF664873BO | Recombinant Bovine Metalloendopeptidase OMA1, mitochondrial (OMA1) |
in vitro E.coli expression system |
CSB-YP664873BO1 CSB-EP664873BO1 CSB-BP664873BO1 CSB-MP664873BO1 CSB-EP664873BO1-B |
Recombinant Bovine Metalloendopeptidase OMA1, mitochondrial (OMA1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
OMA1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF856923HU | Recombinant Human Metalloendopeptidase OMA1, mitochondrial (OMA1) (Active) |
in vitro E.coli expression system |
CSB-YP856923HU1 CSB-EP856923HU1 CSB-BP856923HU1 CSB-MP856923HU1 CSB-EP856923HU1-B |
Recombinant Human Metalloendopeptidase OMA1, mitochondrial (OMA1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-MP856923HU | Recombinant Human Metalloendopeptidase OMA1, mitochondrial (OMA1)-VLPs |
Mammalian cell |