Bovine Interferon γ ,IFN-γ ELISA Kit

Code CSB-E09812b
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine IFNG ELISA Kit was designed for the quantitative measurement of Bovine IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Target Name interferon, gamma
Alternative Names IFNG ELISA Kit; Interferon gamma ELISA Kit; BoIFNG ELISA Kit; IFN-gamma ELISA Kit
Abbreviation IFNG
Uniprot No. P07353
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-95  
1:2 Average % 102  
Range % 99-105  
1:4 Average % 94  
Range % 89-97  
1:8 Average % 97  
Range % 93-101  
Recovery
The recovery of bovine IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-96  
EDTA plasma (n=4) 95 92-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 1.974 1.882 1.928 1.785  
1000 1.290 1.196 1.243 1.100  
500 0.818 0.807 0.813 0.670  
250 0.519 0.502 0.511 0.368  
125 0.324 0.333 0.329 0.186  
62.5 0.268 0.280 0.274 0.131  
31.25 0.212 0.221 0.217 0.074  
0 0.142 0.144 0.143    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Gene References into Functions
  1. Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-gamma production via PD-1/PD-L1 blockade in bovine mycoplasmosis. PMID: 28544524
  2. The expression of multiple toll-like receptors, interferon-gamma, and interleukin-12 (IL-12) in cattle with low and high proviral loads of bovine leukemia virus are reported. PMID: 27473994
  3. Diet-driven interferon-gamma enhances malignant transformation of primary bovine mammary epithelial cells through nutrient sensor GCN2-activated autophagy. PMID: 26962688
  4. Data suggest that luteolytic factors (such as IFNG, tumor necrosis factor alpha, and PGF2a) control expression of MMP1, other matrix metalloproteinases, and tissue inhibitors of metalloproteinase in cultured luteal cells. PMID: 25924700
  5. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
  6. These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
  7. Genetic characterization of IFNG gene was done in resistant and susceptible animals of Sahiwal cattle (n = 95) and Friesian (n = 92). PMID: 21960011
  8. Data suggest that activation of gammadelta T cells to IFN-gamma production, NK cell-like killing plays a pivotal role in controlling virus infection. PMID: 21383249
  9. The differential expression levels of IFN-gamma mRNA between cattle and buffalo could be due to a conserved 4 base (GTCT) deletion in the promoter region of buffalo. PMID: 20541234
  10. SNPs in IFNG, IFNGR1 and R2, IL22, and IL22RA1 were analyzed for an association to Estimated breeding values for somatic cell score in Canadian Holstein bulls; no significant associations were found. PMID: 21328101
  11. Nuclear localization sequence of bovine gamma-interferon provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus PMID: 21268843
  12. The upregulation of TNFRI mRNA expression by IFNG suggests that TNF and IFNG synergistically affect the death of luteal endothelial cells resulting in acute luteolysis PMID: 20562522
  13. Data demonstrated that the monoclonal antibodies secreted by the four hybridoma cell lines could react specifically to the recombinant BovIFN-gamma. PMID: 17366886
  14. The entire bovine IFN-gamma gene (BoIFNG) and 2605 bp of its promoter DNA were sequenced. A comprehensive survey for polymorphisms in the bovine IFN-gamma gene reveals a highly polymorphic intronic DNA sequence allowing improved genotyping of Bovinae. PMID: 12396714
  15. Bovine interferon gamma demonstrates no effect on P-glycoprotein transport activity in human Caco-2 intestinal epithelial cells with rhodamine 123 as susbstrate. PMID: 15124210
  16. In giant cell arteritis, IFN-gamma functional polymorphisms are associated with clinical manifestations of severity rather than susceptibility to this vasculitis. PMID: 15675129
  17. The majority of patients with gad-enhancing lesions showed PLP/IFN gamma and MBP/IFN gamma recurrent burst responses PMID: 15957503
  18. A polymorphism within the interferon gamma gene is a risk factor in severe acute respiratory syndrome susceptibility. PMID: 16672072
  19. Intradermal sensitization of cows in the subclinical stage of M. paratuberculosis infection will upregulate expression of interferon gamma, enhancing the sensitivity of this assay. PMID: 17459831
  20. This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. PMID: 17524495
  21. Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
  22. Taken together, these results support the hypothesis of an inactivating phosphorylation of nNOS by IFNgamma, via JAK/STAT, in bovine chromaffin cells. PMID: 19141072
  23. A tendency toward statistical significance for the effect of polymorphisms in the odds of infection in cattle was not found for alleles IFNG. PMID: 19525022

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Subcellular Location Secreted.
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: bta:281237

STRING: 9913.ENSBTAP00000016634

UniGene: Bt.188

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