Interferon gamma (IFN-gamma) is a key cytokine produced primarily by activated T helper 1 cells, cytotoxic T lymphocytes, and natural killer cells. This pleiotropic molecule plays important roles in immune regulation, including macrophage activation, enhancement of antigen presentation, promotion of Th1 cell differentiation, and coordination of cellular immune responses against intracellular pathogens and tumors. IFN-gamma also shows antiviral properties and influences various physiological processes including cell growth regulation and apoptosis, making it a valuable biomarker in immunological and inflammatory research.
The Hamster interferon, gamma (IFNG) ELISA kit (CSB-EL011050HA) provides quantitative measurement of IFN-gamma in Mesocricetus auratus samples using a sandwich assay principle. This kit works with serum, plasma, and tissue homogenate samples with a detection range of 1.87 pg/mL to 120 pg/mL and sensitivity of 0.46 pg/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of hamster IFN-gamma levels.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in immunotherapy and cancer research studies using Syrian hamster models to quantify interferon gamma production. The kit allows measurement of this key cytokine in cell culture supernatants and tissue homogenates to determine immune responses and therapeutic efficacy.
• Cancer immunotherapy research: Measuring interferon gamma levels in splenocyte cultures following various treatment combinations including checkpoint inhibitor therapy and viral vector-based interventions
• Immune cell activation studies: Determining cytokine production by lymphocytes cocultured with tumor cells to measure T-cell responses and characterize Th1/Th2 immune profiles
• Respiratory disease research: Quantifying interferon gamma in lung tissue homogenates alongside other inflammatory mediators to profile cytokine expression patterns
• Therapeutic intervention monitoring: Measuring interferon gamma as a biomarker to determine the immunomodulatory effects of experimental treatments in hamster disease models
