Human urokinase plasminogen activator,uPA ELISA kit

Instructions
Code CSB-E04751h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name urokinase plasminogen activator,uPA
Alternative Names ATF ELISA Kit; ATF uPA ELISA Kit; BDPLT5 ELISA Kit; Plasminogen activator ELISA Kit; Plasminogen activator urinary ELISA Kit; Plasminogen activator urokinase ELISA Kit; PLAU ELISA Kit; QPD ELISA Kit; u PA ELISA Kit; U plasminogen activator ELISA Kit; u-PA ELISA Kit; U-plasminogen activator ELISA Kit; uPA ELISA Kit; URK ELISA Kit; UROK_HUMAN ELISA Kit; Urokinase plasminogen activator ELISA Kit; Urokinase type plasminogen activator ELISA Kit; Urokinase type plasminogen activator precursor ELISA Kit; Urokinase-type plasminogen activator chain B ELISA Kit
Abbreviation uPA
Uniprot No. P00749
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, urine
Detection Range 78.125 pg/mL-5000 pg/mL
Sensitivity 55.100 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of human uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Gene References into Functions
  1. The combination of decoy receptor 3, soluble urokinase type plasminogen activator receptor, and procalcitonin improved the sensitivity and specificity of diagnosis of sepsis, suggesting that use of the combination of three indexes enhanced the efficiency of sepsis diagnosis. PMID: 29760745
  2. High Expressions of PLAU is associated with lung adenocarcinoma. PMID: 28454121
  3. Increased expression of uPA in epidermal cells in psoriasis and in tumor cells in basal cell carcinomas suggests an important role of the uPA system for aggressively proliferating and invading cells of epidermal origin. PMID: 28429105
  4. study provides strong support in the role of L. reuteri in suppression of GC cell invasion by downregulation of pathways which is involved in extracellular matrix degradation such as uPA and uPAR PMID: 28643742
  5. Gelsolin enhances the invasive capacity of colon cancer cells via elevating intracellular superoxide (O2.-) levels by interacting with Cu/ZnSOD, and gelsolin gene expression positively correlates with urokinase plasminogen activator (uPA), an important matrix-degrading protease invovled in cancer invasion. PMID: 27391159
  6. Study provides evidence that the stimulation of u-PA/u-PAR system contributes to the activated phenotype and function of cancer-associated fibroblasts during multiple myeloma. PMID: 28340565
  7. OB-Rb, RhoA/ROCK, PI3K/AKT, JAK/STAT pathways and NF-kB activation are involved in leptin-induced upA expression. PMID: 28104444
  8. Results provide evidence that uPA and IGF1R directly interact with uPAR enhancing malignant potential of triple-negative breast cancer. PMID: 27502396
  9. suggest that the low endogenous levels of uPA in blood are actively regulated, and that the regulatory mechanisms are disrupted in QPD in a megakaryocyte-specific manner PMID: 28301587
  10. an intricate link between caveolin-1 and Src kinase-mediated cell signaling and alveolar epithelial cell apoptosis due to loss of SP-C expression through p53 and uPA system-mediated cross-talk, is reported. PMID: 28385810
  11. results show that the uPA/uPAR/LRP1 system is a potential target for the development of therapeutic strategies to promote axonal recovery following a CNS injury PMID: 27986809
  12. The present research concluded that aspirin suppressed prostate cancer cell invasion by reducing MMP-9 activity and uPA expression through decreasing of IKK-beta-mediated NF-kappaB activation, indicating that the ability of aspirin to inhibit cell invasion might be useful in the chemoprevention of metastatic prostate cancer. PMID: 28278500
  13. These studies identify uPA-dependent de-repression of vegfr1 and vegfr2 gene transcription through binding to HHEX/PRH as a novel mechanism by which uPA mediates the pro-angiogenic effects of VEGF and identifies a potential new target for control of pathologic angiogenesis. PMID: 27151212
  14. The up-regulation of uPA mRNAs was correlated with high-risk clinicopathological features, including extrathyroid invasion, loss of cellular polarity/cohesiveness, and the BRAF(V600E) mutation. PMID: 25085839
  15. Transplantation of uPA gene modified mesenchymal stem cells suppressed liver fibrosis and ameliorated liver function and may be a new approach to treating liver fibrosis PMID: 26877613
  16. ApaL1 and Taq1 single nucleotide polymorphisms of the urokinase and VDR genes are associated with recurrent urolithiasis in a Caucasian population. PMID: 26275878
  17. Resveratrol inhibited hypoxia-induced HIF-1alpha protein expression. Resveratrol also suppressed hypoxiainduced expression of metastatic-related factors, uPA and MMP2. PMID: 26707376
  18. Significance of the urokinase-type plasminogen activator and its receptor in the progression of focal segmental glomerulosclerosis in clinical and mouse models. PMID: 26846181
  19. Data suggest that enhanced levels of uPA in breast cancer modulate the mitogenic effects of EGF which helps to better understand breast cancer pathogenesis. PMID: 25641046
  20. Results found high levels of uPA and uPAR exclusively in metastatic osteosarcoma (OS)cells and suggest that malignant conversion of OS cells to uPA/uPAR axis in an autocrine and paracrine fashion. PMID: 26317203
  21. The morphologically normal tissue adjacent to the tumor shows the substantial expression of MMP-2 and MMP-9 and in some cases the enhanced activity of uPA and ACE, which makes an additional contribution to the increased invasive potential of tumor PMID: 26978018
  22. it was found that the recombinant fusion protein uPA17-34-KPI(kunitz-type protease inhibitor ) inhibited the invasion and metastasis of ovarian tumors PMID: 26166362
  23. Crystal structure of uPA bound with cyclic peptidic inhibitors. PMID: 25744057
  24. u-PA is a dysregulated gene involved in the tumorigenesis, bio-pathological features and outcomes of EEC. PMID: 26033187
  25. High cytoplasmic expression of uPA is associated with cells of rectal cancer and metastases of perienteric lymph nodes. PMID: 26504024
  26. DIM can influence the cell migratory and invasive properties of human colorectal cancer cells and may decrease the invasive capacity of colorectal cancer through downregulation of uPA and MMP9 mediated by suppression of the transcription factor FOXM1 PMID: 25962429
  27. Porphyromonas gingivalis-derived RgpA-Kgp complex activates the macrophage uPA. PMID: 25979345
  28. SNP P141L associated with collateral circulation in coronary artery disease PMID: 24952395
  29. Taking uPA(1-43) amino acids specifically binding to uPAR as targeted part of fusion protein, and making use of antitumor activity of melittin, the recombinant fusion protein it was able to inhibit growth of ovarian tumors . PMID: 25394558
  30. uPA/uPAR stimulates triglyceride synthesis in Huh7 hepatoma cells via p38-dependent upregulation of DGAT2 PMID: 25244504
  31. Studied urokinase-type plasminogen activator regulation in cigarette smoke extract (CSE)-induced EMT in human small airway epithelial cell lines (HSAEpiCs)and in the small airway epithelium of COPD patients. PMID: 25706093
  32. Silencing of ETV4 suppresses invasion of prostate cancer cells by inhibiting the expression of uPA/uPAR as well as (MMP)-2 and MMP-9 PMID: 25544710
  33. miR-193b inhibits the expression of stathmin 1 (STMN1) and urokinase-type plasminogen activator (uPA) in Panc-1 cells. PMID: 25215905
  34. Activation of uPA and MMP-3 in perifocal zone of the tumor can serve as an indexof its invasive activity. PMID: 25993872
  35. These data support the hypothesis that uPA upregulation stimulated by macrophages may play an active role in the onset of osteoarthritis and in the shear-stress protection against this induction. PMID: 23597113
  36. High preprocedural plasma uPA and serum CRP levels are indicative of angina recurrence after successful PTCA, and are valuable for the prognosis of restenosis. PMID: 24057344
  37. Data indicate that urokinase-type plasminogen activator (uPA) activates epithelial sodium channel, gamma subunit (ENaC gamma) through catalytic activity-dependent proteolytic modification. PMID: 25555911
  38. IL-17 may act as chemotropic factor for peripheral blood-Mesenchymal stem cellss by incrementing cell motility and uPA expression during inflammation development. PMID: 25433194
  39. Suggest a possible protective effect of podocyte uPA/uPAR expression against interstitial fibrosis in IgA nephritis. PMID: 24648800
  40. High u-PA expression is associated with metastasis in breast cancer. PMID: 24610082
  41. In human coronary vascular smooth muscle, uPA induces uPAR-independent, domain-dependent smooth muscle cell proliferation through transactivation of EGFR by a plasmin-mediated, ADAM-induced, and HB-EGF-dependent process. PMID: 25082749
  42. IL-33 is involved in u-PA-driven angiogenesis, a process that has been shown before to be linked to inflammation in various pathologies. PMID: 24702774
  43. IL-1beta-induced urokinase plasminogen activator expression via activation of the ERK1/2 and NF-kappaB pathways, which results in invasion of gastric cancer cells. PMID: 24626561
  44. Studied the relationship between uPA gene polymorphisms and clinical manifestations of idiopathic membranous nephropathy. PMID: 24822208
  45. These effects were mediated by the efficient conversion of pro-uPA to active uPA and high phosphorylation levels of ERK detected in the PANC-1 cells expressing exogenous polyserase-1. PMID: 24756697
  46. We found no association between allele frequency and risk of multiples sclerosis for any single nucleotide polymorphism investigated for PLAU PMID: 23897640
  47. results of this study suggest that uPA decreases the removal of HDL-cholesteryl ester in the liver via suppression of the hepatic scavenger receptor class B type I (SR-BI)expression PMID: 24529115
  48. Oncogenic K-Ras and loss of Smad4 mediate invasion by activating an EGFR/NF-kappaB Axis that induces expression of MMP9 and uPA in human pancreas progenitor cells. PMID: 24340014
  49. The findings provide evidence that the rs2227564 C/T SNP in the u-PA gene is associated with the development of oral tongue squamous cell carcinoma. PMID: 23621237
  50. Decreasing uPA levels sensitized cancer cells to TRAIL, leading to markedly increased apoptosis. PMID: 24481457
  51. Plasminogen stimulates airway smooth muscle cell proliferation in a manner mediated by urokinase and annexin A2. PMID: 24111848
  52. The uPA expression was significantly correlated with TMPRSS4 expression in human lung and prostate cancers. PMID: 23978400
  53. uPAR, IL-33, and ST2 values as a predictor of subclinical chorioamnionitis in preterm premature rupture of membranes. PMID: 24304137
  54. we are reporting the strong correlation among uPA and ECM proteins in newly diagnosed MM patients of different stages. PMID: 24807734
  55. The uPA/uPAR angiogenesis regulatory system supports the endothelial cells that need to migrate, invade the extracellular matrix, survive, proliferate, and to re-establish new contacts with cells and the surrounding matrix. (Review) PMID: 24217605
  56. The effects of UPA/UPAR on migration of lipid-loaded cells occurred through the binding to LRP-1. PMID: 23812296
  57. Urokinase plasminogen activator is a central regulator of macrophage three-dimensional invasion, matrix degradation, and adhesion. PMID: 24616477
  58. TMPRSS4 directly converted inactive pro-uPA into the active form through its proteolytic activity. Conditioned medium from cells overexpressing TMPRSS4 showed that the active TMPRSS4 protease domain is released and is associated with the plasma membrane. PMID: 24434139
  59. The expression of the uPA system positively correlates with peritoneal metastasis of gastric cancer. PMID: 23985164
  60. T allele of rs2227564 polymorphism in PLAU gene could increase the effects on risk of Alzheimer's disease PMID: 23813610
  61. Analysis revealed a positive correlation between u-PA and MMPs and a negative correlation between u-PA/MMPs and TIMPs. PMID: 23900236
  62. Results show that uPA regulates Lhx2 expression by suppressing expression of miR-124 and p53 expression. These results demonstrate that regulation of gene transcription by uPA contributes to pancreatic cancer cell stemness and clinical lethality PMID: 23864708
  63. Matrix metalloproteinases (MMP)--MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines PMID: 24479343
  64. Analysis revealed a positive correlation between u-PA and MMPs and a negative correlation between u-PA/MMPs and TIMPs. These findings suggest the therapeutic potential of NM in treatment of adult sarcomas PMID: 23661254
  65. Urokinase plasminogen activator induces pro-fibrotic/m2 phenotype in murine cardiac macrophages. PMID: 23536772
  66. Data indicate that knockdown of interleukin enhancer-binding factor 3 (ILF3) leads to the increased levels of mature urokinase-type plasminogen activator (uPA) mRNA-targeting miRNAs miR-193a, miR-193b and miR-181a. PMID: 22986534
  67. uPA has a major role in cholesterol biosynthesis; PLAU Pro141Leu (rs2227564) is significantly associated with serum lipid levels in a Japanese population PMID: 23628797
  68. The beta sheet in the growth factor-like domain is not necessary for high affinity binding of uPA to uPAR. PMID: 23848154
  69. In conclusion, our data suggest that radiation-mediated hypomethylation triggers uPA expression in meningioma cells. PMID: 23441133
  70. P141L and 3'-UTR polymorphisms of the urokinase gene are not associated with urolithiasis in a Japanese population PMID: 23532423
  71. Dose-dependent increases in uPA and IL-8 expression were detected following TRAIL stimulation in pancreatic ductal adenocarcinoma cells. PMID: 23392805
  72. study investigated the role of uPA, PAI-1, MMP-2, and TIMP-2 in formation of self-assembled network in the 2D co-ulture of bone marrow stromal cells and HUVECs; data showed that uPA played an important role in network formation, while the process appears independent on TIMP-2 but involved MMP-2 PMID: 23059760
  73. urokinase plasminogen activator is involved in mediating the tumor-suppressing effects of CFTR PMID: 22797075
  74. UPA and VEGF protein expression of the high microvessel density (MVD) group was significantly lower than in the low MVD group (P < 0.05). PMID: 22994764
  75. these data suggest that PKD2 and PKD3 coordinate to promote prostate cancer cell invasion through p65 NF-kappaB- and HDAC1-mediated expression and activation of urokinase-type plasminogen activator (uPA). PMID: 22797919
  76. SphK1 promoting the secretion of MMP-2/9 and uPA via activation of ERK1/2 and suppression of p38 MAPK pathways maybe the molecular mechanisms for its regulation of the malignant behavior of colon cancer cell. PMID: 22684547
  77. UVA stimulated the production of uPA, a key factor in the modulation of extracellular matrixes, inflammatory processes, and angiogenesis. PMID: 23322578
  78. In papillary thyroid carcinoma BRAFV600E is associated with increased expression of the urokinase plasminogen activator and its cognate receptor, but not with disease-free interval. PMID: 22702340
  79. The detrimental effects of atherogenic LDL on VSMC are mediated by a decrease and delocalization of the UPA-UPAR interaction that result in an impairment of cytoskeleton dynamics and adhesion capacity affecting cell phenotype and function. PMID: 22906080
  80. MMP-9 and uPA might be involved in the activation of pro-MMP-13 through unknown mechanisms in arthritic diseases. PMID: 21913037
  81. Complexes between alpha2-macroglobulin and urokinase were detected in alveolar edema fluids as well as in lungs of patients with acute lung injury/acute respiratory distress syndrome where they were located mainly in close proximity to epithelial cells. PMID: 23064953
  82. the effect of the mu-opioid receptor selective peptide, morphiceptin, and its two synthetic analogs on mRNA and protein levels of MMP-9 PMID: 23350235
  83. novel functions of gelsolin in colorectal tumor cell invasion through its modulation of the uPA/uPAR cascade PMID: 22927998
  84. PLAU is particularly important for memory Tregs and PLAU mediates Treg suppressor function via STAT5 and ERK signaling pathways. PMID: 23169000
  85. aminoterminal fragment requires NAD(P)H oxidase activity through a Gbetagamma-, rac-, and src-mediated pathway to facilitate transactivation of EGFR and vascular smooth muscle cell migration PMID: 22575880
  86. Data suggest that lycopene, a dietary component, inhibits angiogenesis; lycopene appears to down-regulate signal transduction by inhibition of uPA and MMP2 (matrix metallopeptidase 2) via vascular endothelial growth factor receptor 2 signaling. PMID: 22707264
  87. Small hairpin RNA against uPA and/or uPAR increased secretion of TIMP-1, which is known to enhance SVEGFR1 secretion in endothelial cells and glioblastoma cells. PMID: 22177802
  88. TGF-beta1-induced activation of Smad3 is the critical step for the uPA upregulation and E-cadherin downregulation PMID: 21798735
  89. The downregulation of uPA/uPAR results in increased integrin alphavbeta5 cell-surface protein levels that regulate the activity of beta1 integrins, promoting characteristics of the persistent Myofibroblasts. PMID: 22470492
  90. HDAC inhibitor trichostatin A (TSA) increased the expression of TIMP-1 and TIMP-3 while decreased MMP-2, MMP-9 and uPA and have an inhibitory effect on trophoblast invasion. PMID: 22291969
  91. In the Brazilian population, genetic variations in both uPA and TAFI were not relevant to endometriosis and/or infertility. PMID: 21819230
  92. suggest that uPA promotes cell migration by binding to fibulin-5, initiating its cleavage by plasmin, which leads to its dissociation from beta1-integrin and thereby unblocks the capacity of integrin to facilitate cell motility PMID: 22280367
  93. The effect of SDF-1 on colorectal cancer cell signaling and uPA expression is mediated by the CXCR4/beta1 integrin axis. PMID: 21567400
  94. Confirmed the correlation between elevated uPA values & an aggressive course of invasive breast cancer. PMID: 21989445
  95. Posttranscriptional regulation of uPA involves the newly recognized pathway of approximately 40 kDa cytoplasmic-nuclear shuttling protein ribonuclease reductase M2 (RRM2) present in the uPA mRNA 3'untranslated region (UTR). PMID: 22166006
  96. Data show that urokinase plasminogen activator expression correlates with lymphatic invasion and metastasis in vivo and is required for CCA cell invasion in vitro, suggesting its potential as a therapeutic target. PMID: 22294827
  97. Data show that kahweol inhibits HUVEC MMP-2 and urokinase involved in angiogenesis. PMID: 21858104
  98. Studies indicate that the genetic cause of QPD is a tandem duplication mutation of the uPA gene, PLAU, which upregulates uPA expression in megakaryocytes by an unknown mechanism. PMID: 22102275
  99. survivin is an essential mediator of arthritogenic properties of uPA regulating its synthesis in synovial fibroblasts and uPAR expression in leucocytes PMID: 19298527
  100. No association between uPA (rs4065)polymorphism and endometrial carcinoma risk PMID: 21761413

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Involvement in disease Quebec platelet disorder (QPD)
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Expressed in the prostate gland and prostate cancers.
Database Links

HGNC: 9052

OMIM: 191840

KEGG: hsa:5328

STRING: 9606.ENSP00000361850

UniGene: Hs.77274

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