Mouse Complement 5,C5 ELISA Kit

Instructions
Code CSB-E13802m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Complement 5,C5
Alternative Names C5 ELISA Kit; HcComplement C5 ELISA Kit; Hemolytic complement) [Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain] ELISA Kit
Abbreviation C5
Uniprot No. P06684
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.25 ng/mL-16 ng/mL
Sensitivity 0.06 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %93
Range %86-100
1:200Average %95
Range %87-106
1:400Average %90
Range %86-99
1:800Average %95
Range %90-105
Recovery
The recovery of mouse C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9687-101
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
162.011 1.964 1.988 1.827
81.589 1.564 1.577 1.416
41.095 1.038 1.067 0.906
20.643 0.620 0.632 0.471
10.442 0.426 0.434 0.273
0.50.297 0.285 0.291 0.130
0.250.221 0.216 0.219 0.058
00.156 0.165 0.161
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; FUNCTION
Gene References into Functions
  1. In mice that lost the ability to express complement C5, there was a lower frequency of metastasis, and males no longer had a higher frequency of metastasis than females. PMID: 27105526
  2. C5 contributes effectively to liver steatosis and inflammation in non-alcoholic fatty liver disease pathogenesis PMID: 27477770
  3. C5a in vitro caused activation (phosphorylation) of MAPKs and Akt in cardiomyocytes, which required availability of both C5a receptors. These data suggest that polymicrobial sepsis causes cardiac dysfunction that appears to be linked to activation of MAPKs and Akt in heart. PMID: 28572445
  4. data suggest a novel role for the anaphylatoxin C5a as a master switch of the delicate pHi balance in neutrophils resulting in profound inflammatory and metabolic changes that contribute to hyperlactatemia during sepsis. PMID: 28490576
  5. n the complex but clinically relevant DH model the local and systemic inflammatory immune response features both, C5-dependent and C5-independent characteristics. Activation of caspase-3 in lung tissue after DH was C5-dependent whereas local inflammation in lung tissue was C5-independent. PMID: 27437704
  6. We induced anti-myeloperoxidase vasculitis in bone marrow chimaeric mice and found that circulating and not bone marrow-derived C5 was required for disease PMID: 27235854
  7. Choroidal neovascularization lesions trigger a systemic immune response, augmenting local ocular inflammation via the infiltration of IL-17-producing gamma-delta T-cells, which are presumably recruited to the eye in a C5a-dependent manner. PMID: 27029558
  8. Carboxypeptidase B2 deficiency reveals that complement C5a exacerbates infection in a murine polymicrobial sepsis model. PMID: 25851247
  9. In mice, loss of C5 or injection of a C5a-receptor antagonist significantly reduced the level of fibrosis of chronic pancreatitis. PMID: 26001927
  10. C5a signaling increases the expression of the chemokine monocyte chemoattractant protein-1 in hepatic metastases of colon cancer PMID: 25739439
  11. C5a produced during lung injury binds to C5aR in alveolar macrophages, initiates downstream signaling that promotes autophagy. PMID: 25032853
  12. Loss of C5a is associated with atherosclerotic plaques. PMID: 25124749
  13. Protein kinase C-delta mediates sepsis-induced activation of complement 5a and urokinase-type plasminogen activator signaling in macrophages PMID: 24682410
  14. Activation of the complement cascade (ComC) and cleavage of C5 by C5 convertase are enabling events in the release of C5a that plays a crucial role in the egress of hematopoietic stem cells from bone marrow. PMID: 24667943
  15. Protective actions of aspirin-triggered (17R) resolvin D1 and its analogue, 17R-hydroxy-19-para-fluorophenoxy-resolvin D1 methyl ester, in C5a-dependent IgG immune complex-induced inflammation and lung injury. PMID: 25172497
  16. The C5(-/-) mice displayed significantly reduced lumenal inflammatory infiltration and cytokine production in oviduct tissue, suggesting that C5 may contribute to chlamydial induction of hydrosalpinx by enhancing inflammatory responses. PMID: 24842924
  17. Complement component C5a contributes to fetal cortical brain injury in a mouse model of preterm birth. PMID: 24184716
  18. C5 plays an important role in glaucoma, with its deficiency lessening disease severity. PMID: 23806181
  19. C5a regulates IL-12(+)dendritic cell migration to induce pathogenic Th1 and Th17 cells in sepsis. PMID: 23936099
  20. lipocalin OmCI ameliorates murine immune complex-induced acute lung injury in a process involving inhibition of LTB4 and C5 PMID: 23625922
  21. component 5a has a role in vascular integrity and airway remodeling PMID: 23530212
  22. C5a generating enzyme included in cowshed dust extract (CDE) might account for some of the allergy protective effects of CDE by generation of C5a in murine lungs. PMID: 23093649
  23. unilateral uterine ischemia/reperfusion results in bilateral fetal loss and fetal growth restriction, mediated by a systemic mechanism. In the current model, this pathological process is completely dependent on intact complement component 5. PMID: 22688254
  24. The presence of C5a in tumor-bearing mice renders naive innate leukocytes more cytotoxic to tumor cells, and indicates C5a has activating potential of naive neutrophils and/or natural killer (NK) cells for superior effector function. PMID: 22914051
  25. C5a robustly suppresses IL-27(p28) gene expression and release in peritoneal macrophages. PMID: 22491257
  26. These results strongly suggest that the complement component 5 (C5) plays a critical role in early protective inflammatory response to infection with Taenia crassiceps. PMID: 22206032
  27. C5 deficiency leads to attenuated inflammation and normalized MMP-9 activity concomitantly with a significant reduction of fibrosis. PMID: 22277671
  28. IL-17F was produced in peritoneal macrophages after TLR4 activation, peaking after 12 h. This effect was completely dependent on the presence of MyD88. The copresence of the complement activation product C5a amplified IL-17F production. PMID: 21859896
  29. Real-time imaging reveals that P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent. PMID: 22199395
  30. P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent. PMID: 22057273
  31. Data demonstrate that high locally generated C5a levels are present in wounds for at least 72 hours after incision. PMID: 21736743
  32. Dysregulation of complement C5 in synovial joints has a key role in the pathogenesis of osteoarthritis. PMID: 22057346
  33. C5a alters blood-brain barrier integrity by signaling through CD88 and thereby disturbs the brain microenvironment. PMID: 21929539
  34. Our results identify C5aR activation as a novel pathway driving detrimental effects of NKT and NK cells during early experimental sepsis. PMID: 22058413
  35. Complement component 5 contributes to poor disease outcome in a pneumococcal meningitis model. PMID: 21926466
  36. Complement C5b-9 induce a JNK/Bid-dependent and JNK-independent necrotic cell death. PMID: 19864026
  37. The development of atypical hemolytic uremic syndrome depends on complement C5. PMID: 21148255
  38. Antibody-mediated blockade of C5, treatment with C5a Receptor antagonist, or C5a Receptor deficiency in mice resulted in enhanced pathological retina angiogenesis. PMID: 20625009
  39. these data provide compelling evidence for a protective role of C5a during bloodstream infection by Staphylococcus aureus. PMID: 20375626
  40. The data suggest that complement activation by exogenous or endogenous stimulation can initiate Th17 cell differentiation and expansion in certain autoimmune diseases and presumably in microbial infections. PMID: 20457757
  41. C5a participates in the regulation of IL-17 secretion by gammadelta T cells following sepsis after cecal ligation and puncture. C5a acted on DC, which subsequently induced T cells to secrete IL-17. PMID: 20140904
  42. Complement Ca activates expression of the mRNA encoding cyclooxygenase 2 but not cyclooxygenase 1 in the hypothalamus. PMID: 19800413
  43. function in regulating the inhibitory/activating FcgammaRII/III receptor pair to connect complement and FcgammaR effector pathways in immune inflammation PMID: 12488432
  44. C5 may be required for the limitation of inflammatory response within the central nervous system. PMID: 15033785
  45. Complement C5 may be required in experimental autoimmune encephalomyelitis for clearance of apoptotic inflammatory cells and protection of oligodendrocytes from Fas-dependent apoptosis, possibly through activation of the terminal C5b-9 complex. PMID: 15100315
  46. causative role of C5 deficiency in the dysregulated cytokine response to Candida albicans PMID: 15385488
  47. genome scan provides additional evidence confirming the role of C5 as a probable candidate gene for collagen-induced arthritis 2 and collagen-induced arthritis 4 loci PMID: 15529344
  48. C5 has a causal role in fibrogenesis across species PMID: 15995705
  49. plays a significant role in mediation of chemotactic and activation events during mycobacterial infection PMID: 16101818
  50. data suggest that, in the genetic absence of C3, thrombin substitutes for the C3-dependent C5 convertase to produce C5a; this linkage between the complement and coagulation pathways may represent a new pathway of complement activation PMID: 16715088

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Subcellular Location Secreted
Database Links

KEGG: mmu:15139

STRING: 10090.ENSMUSP00000028233

UniGene: Mm.2168

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