TP53 Monoclonal Antibody

Datasheet
Code CSB-MA0240771A0m
Product Type Monoclonal Antibody
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P04637
Image
  • Immunohistochemical of paraffin-embedded Human lung cancer tissue using CSB-MA0240771A0m at dilution of 1:200.

  • Immunohistochemical of paraffin-embedded Human rectum cancer tissue using CSB-MA0240771A0m at dilution of 1:200.

  • Immunohistochemical of paraffin-embedded Human breast cancer tissue using CSB-MA0240771A0m at dilution of 1:200.

  • All Lanes:Mouse anti human tumor protein p53 Monoclonal antibody at 1μg/ml
    Lane 1:Whole HEK-293 cells Lysates
    Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size:43,38,39,40,34,35,30,24,25KD
    Observed band size:53KD

Immunogen Recombinant Human TP53 Protein
Raised in mouse
Species Reactivity Human
Specificity specific for human TP53 denatured and native forms.
Tested Applications ELISA,WB,IHC;Recommended dilution:WB:1:500-1:5000,IHC:1:50-1:500
Relevance This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism.Mutations in this gene are associated with a variety of human cancers.
Form liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%,protein G purifed
Clonality monoclonal
Alias P53 Cellular Tumor Antigen P53 Mutant Tumor Protein53 P53 Tumor Suppressor Tumor Protein Tumor Suppressor P53
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Gene Names TP53
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 6H5E7
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Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2
Involvement in disease Esophageal cancer (ESCR); Li-Fraumeni syndrome (LFS); Squamous cell carcinoma of the head and neck (HNSCC); Lung cancer (LNCR); Papilloma of choroid plexus (CPP); Adrenocortical carcinoma (ADCC); Basal cell carcinoma 7 (BCC7)
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, Note=Interaction with BANP promotes nuclear localization, Recruited into PML bodies together with CHEK2, Translocates to mitochondria upon oxidative stress, Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408), SUBCELLULAR LOCATION: Isoform 1: Nucleus, Cytoplasm, Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4, SUBCELLULAR LOCATION: Isoform 2: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, Cytoplasm, Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells, SUBCELLULAR LOCATION: Isoform 4: Nucleus, Cytoplasm, Note=Predominantly nuclear but translocates to the cytoplasm following cell stress, SUBCELLULAR LOCATION: Isoform 7: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 8: Nucleus, Cytoplasm
Protein Families P53 family
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3
Database Links

HGNC: 11998

OMIM: 133239

KEGG: hsa:7157

STRING: 9606.ENSP00000269305

UniGene: Hs.437460

Pathway MAPK signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Wnt signaling pathway
Apoptosis
Cell cycle
Cellular senescence
Cell ageing and metabolism
Sphingolipid signaling pathway
Neurotrophin signaling pathway

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