Phospho-CCND1 (Ser90) Antibody

Datasheet
Code CSB-PA963558
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P24385
Image
  • Western blot analysis of extracts from K562 tissue using Cyclin D1 (Phospho-Ser90) antibody.The lane on the right is treated with the antigen-specific peptide.
Immunogen Peptide sequence around phosphorylation site of serine 90(F-L-S(p)-L-E) derived from Human Cyclin D1 .
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of Cyclin D1 only when phosphorylated at serine 90.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Sewing A, M?er R (1994) Oncogene 9, 2733-6

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias BCL1; CCND1; CYL-1; PRAD1; PRAD1 oncogene
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Target Names CCND1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Involvement in disease Multiple myeloma (MM)
Subcellular Location Nucleus, Cytoplasm, Membrane
Protein Families Cyclin family, Cyclin D subfamily
Database Links

HGNC: 1582

OMIM: 168461

KEGG: hsa:595

STRING: 9606.ENSP00000227507

UniGene: Hs.523852

Pathway Hedgehog signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Wnt signaling pathway
Focal adhesion
Cell cycle
Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
Apelin signaling pathway
FoxO signaling pathway

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