Name: Recombinant Human RAC-alpha serine/threonine-protein kinase (AKT1)
Brand: CUSABIO
SKU: CSB-EP001553HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

AKT1

Uniprot No.

P31749

Research Area

Apoptosis

Alternative Names

AKT 1; AKT; AKT1; AKT1_HUMAN; C AKT; cAKT; MGC99656; PKB alpha; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-480aa

Target Protein Sequence

MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

82.7kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To produce recombinant human AKT1 in E. coli, the gene of interest is co-cloned into a vector with an N-terminal GST-tag gene and transformed into E. coli cells. The gene of interest encodes the full-length human AKT1 (1-480aa). The cells are cultured to express the AKT1 protein and then lysed to extract it. The harvested protein is purified using affinity chromatography. Its purity is checked with SDS-PAGE, reaching over 90%.

The Human AKT1 protein is a serine/threonine-protein kinase that belongs to the protein kinase B (PKB) family. AKT1 phosphorylates various substrates, indicating its role in diverse cellular processes [1]. It plays a crucial role in various cellular processes such as cell growth, survival, glucose metabolism, genome stability, and neovascularization [2][3]. The phosphorylation-dependent substrate selectivity of AKT1 underscores its specificity in signaling cascades [4]. AKT1 is a key mediator of the PI3K/AKT/mTOR signaling pathway, which is essential for regulating cell proliferation and inhibiting apoptosis, particularly in cancer [5]. Human AKT1 has been identified to have 215 unique human proteins as its experimentally verified substrates, highlighting its significance in cellular signaling networks [6]. The activation of AKT1 is initiated by binding its pleckstrin homology (PH) domain to phosphoinositides, followed by phosphorylation at specific regulatory amino acids, serine 473 and threonine 308 [7].

References:
[1] M. McKenna, N. Balasuriya, S. Zhong, S. Li, & P. O’Donoghue, Phospho-form specific substrates of protein kinase b (akt1), Frontiers in Bioengineering and Biotechnology, vol. 8, 2021. https://doi.org/10.3389/fbioe.2020.619252
[2] Y. Yoshioka, T. Suzuki, Y. Matsuo, M. Nakakido, G. Tsurita, C. Simoneet al., Smyd3-mediated lysine methylation in the ph domain is critical for activation of akt1, Oncotarget, vol. 7, no. 46, p. 75023-75037, 2016. https://doi.org/10.18632/oncotarget.11898
[3] J. Testa and A. Bellacosa, Akt plays a central role in tumorigenesis, Proceedings of the National Academy of Sciences, vol. 98, no. 20, p. 10983-10985, 2001. https://doi.org/10.1073/pnas.211430998
[4] N. Balasuriya, N. Davey, J. Johnson, H. Liu, K. Biggar, L. Cantleyet al., Phosphorylation-dependent substrate selectivity of protein kinase b (akt1), Journal of Biological Chemistry, vol. 295, no. 24, p. 8120-8134, 2020. https://doi.org/10.1074/jbc.ra119.012425
[5] H. Zhao, B. Martin, M. Bisoffi, & D. Dunaway-Mariano, The akt c-terminal modulator protein is an acyl-coa thioesterase of the hotdog-fold family, Biochemistry, vol. 48, no. 24, p. 5507-5509, 2009. https://doi.org/10.1021/bi900710w
[6] F. Chen, Q. Liu, T. Hilliard, T. Wang, H. Liang, W. Gaoet al., A chemical-genetics and nanoparticle enabled approach for in vivo protein kinase analysis,, 2020. https://doi.org/10.1101/2020.05.13.094573
[7] A. Kumar, V. Rajendran, R. Sethumadhavan, & R. Purohit, Akt kinase pathway: a leading target in cancer research, The Scientific World Journal, vol. 2013, p. 1-6, 2013. https://doi.org/10.1155/2013/756134

Read more reviews >>

■ Q&A

I am interested in several of your recombinant proteins. Can you please provide some informations:
For CSB-EP001553HU (all expression systems):
a.Sequence
b.Tag type
c.Tag position
d.Sizes and formats
e.Is the protein provided soluble in solution?
f.Is the protein purified from bacterial inclusion bodies are you supplied refolded or still in urea?
g.I want to use this protein for conjugation purposes and need it supplied in an amine free buffer (so not Tris). Can you supply this protein in one of the buffers at my request: phosphate, carbonate, or borate? Would there be an extra charge?

Thanks for your inquiry. Proteins will be supplied soluble in solution.
It is not certain for the protein to be expressed in the supernatant or precipitation, but our proteins are provided with folding correctly.
If you need it supplied in an amine free buffer (so not Tris). We can do this. Please mention this requirement in your official order. We can try to provide PBS buffer, this is not extra charged.
Recombinant Human RAC-alpha serine/threonine-protein kinase(AKT1)
CSB-YP001553HU
CSB-EP001553HU
CSB-BP001553HU
CSB-MP001553HU
Expression Region: 1-480aa;Full length
Tag information:EP: N-terminal GST-tagged;YP, BP, MP:Tag type will be determined during the manufacturing process.
The expected tag for other expression systems are listed as follows:YP: N-terminal 6xHis-tagged;BP,MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
You can view the full sequence on this page.

Target Background

Function

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53. Phosphorylates palladin (PALLD), modulating cytoskeletal organization and cell motility. Phosphorylates prohibitin (PHB), playing an important role in cell metabolism and proliferation. Phosphorylates CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. Phosphorylates PCK1 at 'Ser-90', reducing the binding affinity of PCK1 to oxaloacetate and changing PCK1 into an atypical protein kinase activity using GTP as donor. Also acts as an activator of TMEM175 potassium channel activity in response to growth factors: forms the lysoK(GF) complex together with TMEM175 and acts by promoting TMEM175 channel activation, independently of its protein kinase activity.

Gene References into Functions

The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway. PMID: 29410498
Results suggest that transient receptor potential vanilloid 4 (TRPV4) accelerates glioma migration and invasion through the AKT/Rac1 signaling, and TRPV4 might be considered as a potential target for glioma therapy. PMID: 29928875
Data indicate a regulatory mechanism underlying drug resistance and suggest that tribbles homologue 2 (TRIB2) functions as a regulatory component of the PI3K network, activating AKT in cancer cells. PMID: 28276427
findings indicated that shikonin inhibits proliferation and promotes apoptosis in human endometrioid endometrial cancer (EEC) cells by modulating the miR-106b/PTEN/AKT/mTOR signaling pathway, suggesting shikonin could act a potential therapeutic agent in the EEC treatment. PMID: 29449346
SIRT6 inhibited proliferation, migration, and invasion of colon cancer cells by up-regulating PTEN expression and down-regulating AKT1 expression. PMID: 29957460
LHPP suppresses cell proliferation and metastasis in cervical cancer, and promotes apoptosis by suppressing AKT activation. PMID: 29944886
Data show that activated proto-oncogene protein Akt (AKT) directly phosphorylates Fas associated factor 1 (FAF1) reduces FAF1 at the plasma membrane and results in an increase in TGF-beta type II receptor (TbetaRII) at the cell surface. PMID: 28443643
Data show while overexpression of AKT serine/threonine kinase 1 (AKT1) promoted local tumor growth, downregulation of AKT1 or overexpression of AKT serine/threonine kinase 2 (AKT2) promoted peritumoral invasion and lung metastasis. PMID: 28287129
High AKT1 expression is associated with metastasis in ovarian cancer. PMID: 29739299
Circ-CFH promotes glioma progression by sponging miR-149 and regulating the AKT1 signaling pathway. PMID: 30111766
High AKT1 expression is associated with metastasis via epithelialmesenchymal transition carcinoma in colorectal cancer. PMID: 30066935
High AKT1 expression is associated with tumor-node-metastasis in nonsmall cell lung cancer. PMID: 30106450
High expression of AKT1 is associated with drug resistance and proliferation of breast cancer. PMID: 28165066
Germline variants in AKT1 gene are associated with prostate cancer. PMID: 29298992
High AKT1 expression is associated with cisplatinresistant oral cancer. PMID: 29956797
that Akt1 was a novel target for miR-637, and its knockdown also induced cell growth inhibition and apoptosis in pancreatic ductal adenocarcinoma cells PMID: 29366808
High AKT1 expression is associated with periodontitis. PMID: 30218719
High AKT1 expression is associated with angiogenesis of esophageal squamous cell carcinoma. PMID: 30015941
High AKT1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
In MCF-7 cells, AIB1 overexpression increases p-AKT (Ser 473) activity.In both T47D and MCF-7 cells overexpressing A1B1, p-AKT (Ser 473) expression was significantly increased in the presence or absence of IGF-1,but increased more in presence of IGF-1. PMID: 29808803
In this study, we used the Ion Personal Genome Machine (PGM) and Ion Torrent Ampliseq Cancer panel to sequence hotspot regions from PIK3CA, AKT and PTEN genes to identify genetic mutations in 39 samples of TNBC subtype from Moroccan patients and to correlate the results with clinical-pathologic data PMID: 30227836
AKT pathway is activated by CBX8 in in hepatocellular carcinoma. PMID: 29066512
Here the authors identified a direct interaction of both MEK1 and MEK2 with AKT. The interaction between MEK and AKT affects cell migration and adhesion, but not proliferation. The specific mechanism of action of the MEK-AKT complex involves phosphorylation of the migration-related transcription factor FoxO1. PMID: 28225038
miR-195 suppresses cell proliferation of ovarian cancer cells through regulation of VEGFR2 and AKT signaling pathways. PMID: 29845300
High AKT1 expression is associated with cell growth, aggressiveness, metastasis in gastric cancer. PMID: 30015981
this is the first report showing long-duration exposure to nicotine causes increased proliferation of human kidney epithelial cells through activation of AKT pathway. PMID: 29396723
RBAP48 overexpression contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3kinase/protein kinase B pathway suppression. PMID: 29901205
Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity. PMID: 28209968
PI3K-Akt pathway inhibitors, Akti-1/2 and LY294002, reduced PFKFB3 gene induction by PHA, as well as Fru-2,6-P2 and lactate production. Moreover, both inhibitors blocked activation and proliferation in response to PHA, showing the importance of PI3K/Akt signaling pathway in the antigen response of T-lymphocytes. PMID: 29435871
RIO kinase 3 (RIOK3) positively regulates the activity of the AKT/mTOR pathway in glioma cells. PMID: 29233656
High AKT1 phosphorylation is associated with colorectal carcinoma. PMID: 29970694
Results show that AKT1 was associated with hypertension in Mexican Mestizos but not Mexican Amerindians. PMID: 30176313
TERT could induce thyroid carcinoma cell proliferation mainly through the PTEN/AKT signaling pathway. PMID: 29901196
findings uncover a new function of p53 in the regulation of Akt signaling and reveal how p53, ASS1, and Akt are interrelated to each other. PMID: 28560349
We performed quantitative mass spectrometry of IAV1918-infected cells to measure host protein dysregulation. Selected proteins were validated by immunoblotting and phosphorylation levels of members of the PI3K/AKT/mTOR pathway were assessed. PMID: 29866590
Radiation resistance tumors have upregulated Onzin and POU5F1 expression. PMID: 29596836
The essential role of AKT in the endocrine therapy resistance in estrogen receptor-positive, HER2-negative breast cancer.[review] PMID: 29086897
FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR-637 in HSCR. PMID: 30062828
The overexpression of CHIP significantly increased the migration and invasion of the DU145 cells, which is possible due to activation of the AKT signaling pathway and upregulation of vimentin. The expression level of CHIP was observed to be increased in human prostate cancer tissues compared with the adjacent normal tissue. PMID: 29693147
Genistein (GE) inhibited the growth of human Cholangiocarcinoma (CCA) cell lines by reducing the activation of EGFR and AKT, and by attenuating the production of IL6. E2 and ER were also involved in the growth-inhibitory effect of GE in CCA cells. PMID: 29693152
this study identifies ORP2 as a new regulatory nexus of Akt signaling, cellular energy metabolism, actin cytoskeletal function, cell migration, and proliferation. PMID: 29947926
The role of USP18 in breast cancer provides a novel insight into the clinical application of the USP18/AKT/Skp2 pathway. PMID: 29749454
Collectively, these results indicate that COX-1/PGE2/EP4 upregulates the beta-arr1 mediated Akt signaling pathway to provide mucosal protection in colitis. PMID: 28432343
AKT kinase pathway is regulated by SPC24 in breast cancer. PMID: 30180968
CREBRF promotes the proliferation of human gastric cancer cells via the AKT signaling pathway. PMID: 29729692
these results indicate that miR124 transection inhibits the growth and aggressive of osteosarcoma, potentially via suppression of TGFbetamediated AKT/GSK3beta/snail family transcriptional repressor 1 (SNAIL1) signaling, suggesting miR124 may be a potential anticancer agent/target for osteosarcoma therapy. PMID: 29488603
piperine reduced the expression of pAkt, MMP9 and pmTOR. Together, these data indicated that piperine may serve as a promising novel therapeutic agent to better overcome prostate cancer metastasis. PMID: 29488612
S100A8 gene knockdown reduced cell proliferation in the HEC-1A cells compared with control cells, induced cell apoptosis, inhibited the phosphorylation of protein kinase B (Akt), and induced the expression of pro-apoptotic genes. PMID: 29595187
intact keratin filaments are regulators for PKB/Akt and p44/42 activity, basal and in response to stretch. PMID: 29198699

Hide All

Involvement in disease

Breast cancer (BC); Colorectal cancer (CRC); Proteus syndrome (PROTEUSS); Cowden syndrome 6 (CWS6)

Subcellular Location

Cytoplasm. Nucleus. Cell membrane.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily

Tissue Specificity

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers dur

Database Links

HGNC: 391

OMIM: 114480

KEGG: hsa:207

STRING: 9606.ENSP00000270202

UniGene: Hs.525622

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Code	CSB-EP001553HU
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human RAC-alpha serine/threonine-protein kinase (AKT1)

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage