Product Details

Uniprot No.

P17927

Target Names

CR1

Alternative Names

Complement receptor type 1 (C3b/C4b receptor) (CD antigen CD35), CR1, C3BR

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CD35

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Complement receptor type 1 (CR1/CD35) serves as a critical regulator of complement activation, functioning as the primary receptor for C3b and C4b on erythrocytes, leukocytes, and glomerular podocytes. This membrane glycoprotein plays essential roles in immune complex clearance, complement cascade regulation, and protection against complement-mediated tissue damage. CR1 has emerged as a significant research target in studies of autoimmune diseases, inflammatory conditions, and neurodegenerative disorders, where dysregulated complement activity contributes to pathology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CD35, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental conditions remain stable across long-term studies and multi-site collaborations.

Validation in immunohistochemistry demonstrates reliable CR1 detection in formalin-fixed, paraffin-embedded human tissues. Testing in human tonsil tissue confirms expected staining patterns in lymphoid compartments, while kidney tissue validation aligns with known CR1 expression on glomerular podocytes. Both applications utilized citrate buffer antigen retrieval at pH 6.0 with overnight primary antibody incubation at 4°C, with recommended working dilutions between 1:50 and 1:200 providing flexibility for optimization across different tissue types and detection systems.

Supplied in a glycerol-containing buffer suitable for long-term storage, this antibody supports immunology and stem cell research programs investigating complement biology, immune regulation, and CR1-associated disease mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages. Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation. Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d. Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells.; (Microbial infection) Acts as a receptor for Epstein-Barr virus.

Gene References into Functions

this study correlated phenotype data (CR1 density per erythrocyte (CR1/E), blood soluble CR1 (sCR1)) with genetic data (density/length polymorphisms) in AD patients and healthy controls.. PMID: 30044434
CR1 rs1704660G, rs17047661G and rs6691117G variants were significantly associated with Chagas disease. PMID: 29323238
Meta-analysis validated the association of late onset Alzheimer disease with CR1 (rs6656401) variants. PMID: 29504051
rs6656401 CR1 is not associated with late-onset Alzheimer's disease. PMID: 28477215
CR1 plays a vital role in Alzheimer's disease pathology and disease progression. PMID: 26742530
the effect of CR1 rs3818361 polymorphism on Alzheimer's disease (AD) risk in Chinese cohorts is consistent with the increased risk observed in European AD cohorts. PMID: 26189835
Genetic variation within the CR1 gene is associated with inflammation and the risk of incident coronary artery disease. PMID: 28033544
The distribution of the CR1 alleles, genotypes and haplotypes differed significantly among population in Brazil, Vietnam, India, Republic of Congo and Ghana. PMID: 28520715
CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis. PMID: 27258232
This study demonstrates an up-regulation of complement regulatory proteins, CD35 and CD55 in HIV associated pre-eclamptic compared to normotensive pregnancy. PMID: 27521598
Although B lymphocytes of active systemic lupus erythematosus (SLE) patients express lower level of CR1, the inhibitory capacity of this complement receptor is still maintained and its ligand-induced clustering results in significant inhibition of the main B cell functions, similar to that found in the case of healthy individuals. PMID: 27981054
CyaA binding did not trigger downstream signaling of CR3 in human monocytes and CyaA-catalyzed elevation of cAMP effectively blocked CR3 signaling initiated by a natural ligand. PMID: 26650353
the Putative Role of CR1 in Alzheimer's Disease PMID: 26914463
Knops blood group antigen a/b genotype was associated with increased susceptibility to severe malaria and the b/b genotype was associated with reduced risk of severe malaria. PMID: 25916414
The C4d/CR1 ratio is a simple and quickly determinable biomarker that enables the differentiation between infection and flare-up in febrile SLE patients at initial evaluation. PMID: 26273660
This meta-analysis further supports previous findings that CR1 rs6656401 polymorphism contributes to Alzheimer's Disease susceptibility. PMID: 24878768
Complement receptor type 1 (CR1/CD35) expressed on activated human CD4+ T cells contributes to generation of regulatory T cells PMID: 25742728
These results provide a framework for understanding how loss of CR1 expression on podocytes may contribute to complement-mediated damage in the kidney. PMID: 26260209
findings provide evidence that the A carrier (AG/GG) and the A allele of the ECR1 A3650G polymorphism may be correlated to the pathogenesis of NRDS and, hence, might be involved in the susceptibility to NRDS. PMID: 25494101
THE COMBINATION OF EXPRESSION OF MARKERS CR1 AND CR2 (CD35/CD21) IN DIAGNOSTIC OF B-CELL LYMPHOPROLIFERATIVE DISEASES PMID: 26189291
CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms may not be involved in susceptibility to malaria in Chinese population. PMID: 25683978
In addition to being a risk factor for Alzheimer's disease development, a CR1 SNP appears to be associated with higher rates of medium-term disease progression PMID: 24998857
Data indicate that complement activation promote complement component 3 C3b deposition on native and acetylated LDL and binding to the complement receptor 1 (CR1). PMID: 25349208
A meta-analysis demonstrated significant associations of both the CR1 rs6656401 and CR1 rs3818361 polymorphisms with late-onset Alzheimer's disease susceptibility. PMID: 24996192
CR1 SNPs correlates with specific aspects of cognitive impairment in Alzheimer's disease patients. PMID: 24530172
This study identified nominally significant or suggestive associations between the LOAD-risky CR1 variants and worse Logical Memory immediate recall scores in blacks. PMID: 23643458
Increased CR1 expression was associated with histology classification. PMID: 23464487
CR1 rs7525160 G > C polymorphism was associated with an increased risk of developing non-small cell lung cancer in Chinese population PMID: 24621201
Polymorphism of the complement receptor 1 gene correlates with the hematologic response to eculizumab in patients with paroxysmal nocturnal hemoglobinuria. PMID: 24038027
this is the first study to elucidate the relationship of the L-CR1 transcript with the disease activity of RA patients. PMID: 24433281
Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. PMID: 24214979
Maternal coding variants in complement receptor 1 is associated with spontaneous idiopathic preterm birth. PMID: 23591632
two Alzheimer's disease-associated CR1 variants, rs6656401 and rs3818361, are associated with major recurrent depression in females in a population-based cohort using individuals from the Generation Scotland: Scottish Family Health Study PMID: 22244847
It did not explain (part of) the association of genome wide association top single-nucleotide polymorphisms rs3818361/rs6656401, nor of the CR1copy number variation, with Alzheimer disease in our cohort PMID: 23582656
ATP release following CR1 ligation increases the mobility of the lipid fraction of RBC membranes, which in turn facilitates CR1 clustering, and thereby enhances the binding avidity of complement-opsonized particles to the RBC CR1. PMID: 24022490
Data show that increased CD35, CD46, CD55 and CD59 on neutrophils and monocytes present potent markers of bacterial infection and viral infection. PMID: 23376460
The CD35 antigen bounds gp350/220 and became latently infected when the fusion receptor HLA II was coexpressed. PMID: 23416052
Genome-wide association studies found 3 new susceptibility loci for Alzheimer disease(AD): complement receptor 1,clusterin,and the phosphatidylinositol-binding clathrin assembly protein. These SNPS are not associated with AD in a Polish population. PMID: 23650005
Our findings suggest complex mechanisms underlying the interaction of CR1, APOE, and brain amyloid pathways in Alzheimer disease PMID: 23022416
Complement receptor type 1 (CR1, CD35) is a potent inhibitor of B-cell functions in rheumatoid arthritis patients. PMID: 22962438
We identified rare small events overlapping CR1 and BIN1 in Alzheimer's disease and normal controls with opposite copy number variation dosage. PMID: 23202439
Malaria inhibits surface expression of complement receptor 1 in monocytes/macrophages, causing decreased immune complex internalization. PMID: 23440418
There are fewer mono- or dinucleated, but dramatically more numbers of tri- or polynucleated megakaryocytes in the bone marrow of transgenic Crry mice. PMID: 23390291
Common CR1 variants significantly protect against severe malaria in an endemic area. PMID: 23152904
In conclusion, despite there are some limitations, this meta-analysis indicates that the A allele of the CR1 SNP rs6656401 is significantly associated with LOAD susceptibility in Chinese Han populations. PMID: 22960360
The clustering phenomenon of CR1 in erythrocytes is identified in electron microscopy. PMID: 22374251
our results support that the CR1-S isoform explains the GWAS signals and open a novel prospect for the investigation of CR1-related disease mechanisms. PMID: 22819390
Simultaneous quantitative analysis of CD64 and CD35 expression on neutrophils might be useful to distinguish between bacterial and viral infections in rheumatoid arthritis patients. PMID: 22237765
CR1 (CD35) and CR3 (CD11b/CD18) act in concert for phagocytosis of opsonized Francisella tularensis by human neutrophils. PMID: 22888138
CLU and CR1 were associated with more rapid cognitive decline. PICALM was associated with an earlier age at midpoint of cognitive decline. PMID: 22952074

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Receptors of complement activation (RCA) family

Tissue Specificity

Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.

Database Links

HGNC: 2334

OMIM: 120620

KEGG: hsa:1378

STRING: 9606.ENSP00000356016

UniGene: Hs.334019

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Code	CSB-RA714310A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA714310A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA714310A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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CR1 Recombinant Monoclonal Antibody

Product Details

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Target Background