CD276, CD276 antigen, alias B7H3, B7-H3, 4Ig-B7-H3, is a type I transmembrane protein, a member of the B7 family, which is mainly expressed on the surface of antigen-presenting cells.
Its mechanism of action involves interaction with members of the T cell co-stimulatory molecule family to activate or inhibit T cell activation and proliferation. CD276 may act as a co-stimulatory factor to promote T cell activation, proliferation and cytokine secretion, or it may play a co-inhibitory role in certain adaptive immune responses, causing tumors to evade immune responses and inhibiting NK cell-mediated cell lysis.
CD276 is expressed in a variety of tumors and is associated with tumorigenesis, invasion and metastasis, and is an important immune checkpoint molecule and potential target for tumor immunotherapy.
Recombinant Human CD276 antigen (CD276), partial (Active) (CSB-MP733578HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD276 at 2 μg/ml can bind Anti-CD276 rabbit monoclonal antibody, the EC50 of human CD276 protein is 1.961-2.243 ng/ml.
Recombinant Macaca fascicularis CD276 molecule(CD276),partial (Active) (CSB-MP5140MOV)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD276 at 2 μg/ml can bind Anti-CD276 recombinant antibody(CSB-RA733578MA1HU). The EC50 is 4.299-5.373 ng/mL.
The purity of CD276 was greater than 90% as determined by SEC-HPLC
CD276 Recombinant Monoclonal Antibody (CSB-RA733578MA1HU)
Validated Data
The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 4.299-5.373 ng/mL.
The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP733578HU(F2)) at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC50 is 47.12-54.16 ng/mL.
Overlay Peak curve showing MCF7 cells stained with CSB-RA733578MA1HUMA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
The following CD276 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD276 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004912GA01HU | CD276 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA005320 | CD276 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA745871ESR1HU | CD276 Antibody | Human | ELISA, WB, IHC |
CSB-PA745871ESR2HU | CD276 Antibody | Human | ELISA, IHC |
CSB-RA733578MA1HU | CD276 Recombinant Monoclonal Antibody | Human, Cynomolgus | ELISA, FC |
CD276 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP731567XBE CSB-EP731567XBE CSB-BP731567XBE CSB-MP731567XBE CSB-EP731567XBE-B |
Recombinant Xenopus laevis CD276 antigen homolog (cd276), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD276 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP733578HU CSB-EP733578HU CSB-BP733578HU CSB-EP733578HU-B |
Recombinant Human CD276 antigen (CD276), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-MP733578HU | Recombinant Human CD276 antigen (CD276), partial (Active) | Mammalian cell |
CSB-MP733578HU-B | Recombinant Human CD276 antigen (CD276), partial, Biotinylated | Mammalian cell |
CSB-MP733578HU(F2) | Recombinant Human CD276 antigen (CD276), partial (Active) | Mammalian cell |
CSB-MP733578HU(A4)f4 | Recombinant Human CD276 antigen (CD276)-VLPs | Mammalian cell |
CD276 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP745871RA CSB-EP745871RA CSB-BP745871RA CSB-MP745871RA CSB-EP745871RA-B |
Recombinant Rat CD276 antigen (Cd276), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD276 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP848693MO CSB-EP848693MO CSB-BP848693MO CSB-EP848693MO-B |
Recombinant Mouse CD276 antigen (Cd276), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CD276 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E14285h | Human Soluble CD276,sCD276/sB7-H3 ELISA Kit | serum, plasma, urine, cell culture supernates | 0.78 ng/mL |