MAPK1 Antibody

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Specification
Target

Code	CSB-PA943928
Product Type	Polyclonal Antibody
Size	US$297
Uniprot No.	P28482
Image	Western blot analysis of extracts from Jurkat cells and 293 cells, using p42 MAPK antibody.
Immunogen	Synthesized peptide derived from internal of Human p42 MAPK.
Raised in	Rabbit
Species Reactivity	Human,Mouse,Rat
Specificity	The antibody detects endogenous levels of total p42 MAPK protein.
Tested Applications	ELISA,WB;WB:1:500-1:3000
Relevance	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992). Gevaert K., Nat. Biotechnol. 21:566-569(2003). Greenway A.L., J. Virol. 70:6701-6708(1996).
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality	Polyclonal
Alias	EC 2.7.11.24; ERK-2; ERK2; ERT1; Extracellular signal- regulated kinase 2
Immunogen Species	Homo sapiens (Human)
Protocols	ELISA Protocol Western Blotting(WB) Protocol
Target Names	MAPK1
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).
Subcellular Location	Cytoplasm, cytoskeleton, spindle, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, Membrane, caveola
Protein Families	Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links	HGNC: 6871 OMIM: 176948 KEGG: hsa:5594 STRING: 9606.ENSP00000215832 UniGene: Hs.431850
Pathway	cAMP signaling pathway Chemokine signaling pathway ErbB signaling pathway Estrogen signaling pathway HIF-1 signaling pathway MAPK signaling pathway Melanogenesis mTOR signaling pathway PI3K-Akt signaling pathway TGF-beta signaling pathway VEGF signaling pathway Focal adhesion Gap junction Lipids and Inflammation in Atherogenesis Regulation of actin cytoskeleton TNF signaling pathway Vascular smooth muscle contraction Apoptosis Autophagy Cellular senescence Ras signaling pathway Signaling pathways regulating pluripotency of stem cells B cell receptor signaling pathway Fc epsilon RI signaling pathway Fc gamma R-mediated phagocytosis IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Apelin signaling pathway cGMP-PKG signaling pathway FoxO signaling pathway Insulin signaling pathway Oxidative Phosphorylation Phospholipase D signaling pathway Rap1 signaling pathway Sphingolipid signaling pathway Excitatory synapse pathway Glutamatergic synapse Neurotrophin signaling pathway

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