Recombinant Sheep Interferon gamma (IFNG) is produced through an E. coli expression system, which appears to provide reliable production of the full-length mature protein spanning amino acids 24 to 166. The protein carries an N-terminal 6xHis-tag that helps with purification and detection processes. This product shows a purity level greater than 90% when verified through SDS-PAGE analysis, suggesting it may be suitable for research applications where high purity matters.
Interferon gamma (IFNG) represents a key cytokine within the immune system. Its primary role involves modulating immune responses, though its effects can be quite complex. The protein activates macrophages and enhances antigen presentation - functions that likely contribute to pathogen defense mechanisms. IFNG also participates in various signaling pathways that regulate immune processes, which is why immunology researchers find it such an interesting subject to study.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. In Vitro Immunological Assays for Sheep-Specific Research
This recombinant sheep IFNG could work as a positive control or standard in ELISA-based assays designed to measure interferon gamma levels in sheep serum, plasma, or cell culture supernatants. Researchers might find it useful as a reference standard when developing species-specific immunoassays or calibrating existing sheep IFNG detection systems. Those studying sheep immune responses, vaccination efficacy, or inflammatory conditions may benefit from using this protein to establish baseline measurements and validate their detection methods.
2. Antibody Development and Characterization
The N-terminal 6xHis-tagged sheep IFNG could serve as an immunogen for generating sheep-specific interferon gamma antibodies in laboratory animals. It might also prove useful for screening existing antibody libraries. The His-tag makes purification and immobilization of the protein on various surfaces relatively straightforward for antibody binding studies, epitope mapping, or antibody affinity measurements. This application appears particularly valuable for researchers developing diagnostic tools or research reagents specific to ovine interferon gamma.
3. Protein-Protein Interaction Studies
Researchers can use the recombinant protein in pull-down assays, taking advantage of the N-terminal His-tag to identify potential binding partners or receptors that interact with sheep interferon gamma. The protein can be immobilized on nickel-affinity matrices to capture interacting proteins from sheep cell lysates or tissue extracts. This approach may help researchers study the molecular mechanisms of interferon gamma signaling pathways in sheep and identify species-specific interaction networks, though results might vary depending on experimental conditions.
4. Comparative Protein Structure and Function Analysis
This sheep IFNG protein could prove useful in comparative studies with interferon gamma from other species to investigate evolutionary relationships, structural differences, and species-specific functional variations. The purified protein can be subjected to biophysical characterization techniques such as circular dichroism spectroscopy, dynamic light scattering, or analytical ultracentrifugation to determine its structural properties. Such studies might contribute to understanding the molecular evolution of cytokines across different mammalian species, though some limitations in cross-species comparisons should be expected.
5. Cell Culture Supplementation for Ovine Cell Lines
Researchers can add the recombinant sheep IFNG to cell culture media containing sheep-derived immune cells or cell lines to study cellular responses in a species-matched system. This application seems particularly relevant for those working with primary sheep immune cells or established ovine cell lines where species-specific cytokine stimulation is required. The protein may be useful for investigating cellular activation, gene expression changes, or metabolic responses in ovine immune cells under controlled laboratory conditions, though optimal concentrations and timing would need to be determined experimentally.
