AK2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA597662A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Hela whole cell lysate(30µg), Mouse kidney tissue lysate(30µg), Rat kidney tissue lysate(30µg), Mouse liver tissue lysate(30µg) All lanes: Adenylate kinase 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time：10s IHC image of CSB-RA597662A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA597662A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA597662A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA597662A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P54819

Target Names

AK2

Alternative Names

Adenylate kinase 2, mitochondrial (AK 2) (EC 2.7.4.3) (ATP-AMP transphosphorylase 2) (ATP:AMP phosphotransferase) (Adenylate monophosphate kinase) [Cleaved into: Adenylate kinase 2, mitochondrial, N-terminally processed], AK2, ADK2

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Adenylate kinase 2 is a mitochondrial enzyme essential for cellular energy homeostasis, catalyzing the reversible transfer of phosphate groups between ATP and AMP. Located in the mitochondrial intermembrane space, AK2 plays a critical role in maintaining adenine nucleotide balance and has been implicated in apoptotic signaling pathways. Mutations in AK2 are associated with reticular dysgenesis, a severe form of immunodeficiency, making this enzyme a significant target for immunology, metabolic disease, and mitochondrial biology research.

This recombinant rabbit monoclonal antibody, clone 7E10, offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human AK2, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, providing confidence across longitudinal studies.

Validation data demonstrates robust performance across multiple platforms. Western blot analysis detects a clean 26 kDa band matching the predicted molecular weight in diverse human cell lines including K562, Jurkat, HEK293, HL-60, and HeLa, with cross-species reactivity confirmed in mouse kidney, mouse liver, and rat kidney tissue lysates. Immunohistochemistry staining has been validated in paraffin-embedded human liver cancer and kidney tissue sections, while immunofluorescence studies in HeLa cells reveal the expected mitochondrial localization pattern. Flow cytometry analysis in Jurkat cells further demonstrates utility for single-cell applications.

This versatile antibody supports researchers investigating mitochondrial function, cellular energetics, immune cell development, and apoptosis mechanisms, offering flexibility across tissue sections, cultured cells, and lysate-based detection methods.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

Gene References into Functions

Genetic variants of AK2 activates tenofovir for HIV therapy. PMID: 29641561
Reticular dysgenesis -patient derived induced pluripotent stem cells can recapitulate disease phenotype which can be rescued by AK2 overexpression. PMID: 29462620
In conclusion, our data suggest that SIRPalpha signaling through SHP-2-PI3K-Akt2 strongly influences osteoblast differentiation from bone marrow stromal cells. PMID: 27422603
AK2 deficiency compromises the mitochondrial energy metabolism required for differentiation of human neutrophil and lymphoid lineages. PMID: 26270350
results suggest that AK2 is an associated activator of DUSP26 and suppresses cell proliferation by FADD dephosphorylation, postulating AK2 as a negative regulator of tumour growth. PMID: 24548998
AK2 is indispensable for neutrophil differentiation, indicating a possible causative link between AK2 deficiency and neutropenia in reticular dysgenesis. PMID: 24587121
These results suggest that, acting in concert with FADD and caspase-10, AK2 mediates a novel intrinsic apoptotic pathway that may be involved in tumorigenesis. PMID: 17952061
The alpha-borano or alpha-H on PMEA and PMPA were detrimental to the activity of recombinant human AMP kinases 2 PMID: 18404568
Biallelic mutations in AK2 (adenylate kinase 2) in seven individuals affected with reticular dysgenesis and sensorineural deafness, were identified. PMID: 19043416
The gene encoding the mitochondrial energy metabolism enzyme adenylate kinase 2 (AK2) is mutated in individuals with reticular dysgenesis. PMID: 19043417

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Involvement in disease

Reticular dysgenesis (RDYS)

Subcellular Location

Mitochondrion intermembrane space.

Protein Families

Adenylate kinase family, AK2 subfamily

Tissue Specificity

Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood,

Database Links

HGNC: 362

OMIM: 103020

KEGG: hsa:204

STRING: 9606.ENSP00000346921

UniGene: Hs.470907

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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