Product Features

● 8 Key Quality Control Standards

CUSABIO ELISA kits are ready-to-use ELISA kits with complete reagents, strict quality control, and validated performance. During the development process, multiple stringent steps are taken to ensure the outstanding quality of the kits, guaranteeing the products provided to customers have high standards in terms of linearity, specificity, stability, and sensitivity.

01
Detection Range

The actual detection sample value is calculated through the standard curve, and the correlation coefficient R of the standard curve for quantitative kits is greater than 0.99.

Take CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) as an example of the standard curve.

ng/ml OD1 OD2 Average Corrected
100 2.839 2.897 2.868 2.718
50 1.968 1.974 1.971 1.821
25 1.144 1.192 1.168 1.018
12.5 0.709 0.733 0.721 0.571
6.25 0.433 0.450 0.441 0.291
3.125 0.303 0.301 0.302 0.152
1.562 0.242 0.223 0.233 0.083
0 0.149 0.151 0.150
CSB-E07270h standard curve
02
Specificity

Specificity is a key indicator of ELISA kits. It is mainly affected by the interference of highly homologous proteins between different species, target protein ligands, or target protein receptors.

CUSABIO selects raw materials with high specificity to ensure the excellent specificity of ELISA kits, analyze cross-reactions and interference, and confirm that only the target analyte can be identified and detected.

03
Lower Detection Limit

The lower detection limit is an indicator of sensitivity. It is determined by factors such as the antibody titer used, and during the development process, the sensitivity and low background detection results are generally optimized to significantly improve the sensitivity of the kit. The lower the detection limit, the higher the sensitivity.

Sensitivity is determined by adding 3 standard deviations to the average O.D. obtained by repeating the zero standard.

04
Linearity

Linearity is a criterion for determining the accuracy and specificity of the kit. To evaluate the linearity of the assay, samples with high concentration analytes are added to various matrices and diluted with sample diluent to produce samples with values within the dynamic range of the assay. Dilution linearity tests are performed for each validated sample type; if the results are within 80%-120% of the expected concentration for each dilution, the linearity is considered good. Linearity is crucial for the accurate measurement of analyte concentrations within the detection dynamic range.

Taking CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) for example: Serum samples are diluted at 1:100, 1:200, 1:400, and 1:800. Cell culture supernatants are diluted at 1:2, 1:4, 1:8, and 1:16. The test values show good linearity within 80-120%.

Sample Serum(n=4)
1:100 Average( %) 91
Range ( %) 85-97
1:200 Average( %) 97
Range ( %) 91-103
1:400 Average( %) 93
Range ( %) 85-95
1:800 Average( %) 95
Range ( %) 90-100
Sample Cell Culture Supernates(n=4)
1:1 Average( %) 95
Range ( %) 83-101
1:2 Average( %) 97
Range ( %) 86-115
1:4 Average( %) 90
Range ( %) 83-98
1:8 Average( %) 91
Range ( %) 80-107
05
Recovery Rate

Recovery rate = measured target protein concentration / added target protein concentration * 100%

It reflects the degree of analyte loss during the sample analysis process; the higher the recovery rate, the higher the accuracy.

Recovery experiments can test whether the kit is affected by interfering factors. Using a natural sample matrix without added protein as a blank control, the recovery rate should be between 80% and 120%, indicating good kit performance.

For example, the recovery rate of CSB-E07270h (Human Adiponectin, ADP ELISA Kit):

Sample Type Average(%) Range(%)
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
06
Accuracy

The coefficient of variation (CV%) represents the consistency of the measurement, i.e., the degree of conformity for repeated measurements of the same sample.

Intra-assay accuracy: The precision testing method within the same batch: measure low, medium, and high-value test samples with the same batch of products 20 times (n) each, calculate the average and standard deviation (SD) of the 20 measurement results, and calculate the coefficient of variation (CV) using the formula CV = standard deviation/average × 100%.

To evaluate the accuracy of CSB-E07270h (Human Adiponectin (ADP) ELISA Kit): CV% <8%.

Inter-assay accuracy: The precision testing method between different batches: measure low, medium, and high-value test samples with three batches of products 20 times (n) each, calculate the average and standard deviation (SD) of the 20 measurement results, and calculate the coefficient of variation (CV) using the formula CV = standard deviation/average × 100%.

To evaluate the accuracy of CSB-E07270h (Human Adiponectin (ADP) ELISA Kit): CV% <10%.

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(ng/ml) 5.345 16.273 29.471 29.471 20.841 34.563
SD 0.24 0.62 0.62 0.43 1.65 2.34
CV(%) 4.49 3.81 6.42 5.14 7.92 6.77
07
Stability

The better the stability, the longer the storage time.

The main components and assay plates of CUSABIO ELISA kits are subjected to heat damage testing at 37°C for 7 days, and then compared with materials stored at 4°C. After 7 days, if the OD value is ≤25%, this is considered satisfactory.

Some reagents can withstand high-temperature heat damage at 60°C, which greatly minimizes the inactivation of antigens and antibodies during long-distance transportation and storage.

08
Natural Sample Validation

CUSABIO ELISA kits can detect natural samples, selecting appropriate natural samples (serum, plasma, cell supernatant, etc.) according to the target properties, providing customers with the most accurate experimental data reference.

Taking CSB-E07270h (Human Adiponectin (ADP) ELISA Kit) as an example, we previously tested serum and plasma samples at a 1:500 dilution, and the test values were S3-S5.

● 15 Research Areas

● 20+ Species

CUSABIO ELISA kits cover more than 20 species, involving multiple research areas, meeting the needs of different customers.

● Wide Range of Detectable Samples

CUSABIO ELISA kits can detect various biological samples, including serum, plasma, body fluids such as urine, cell culture supernatants, and cell/tissue lysates.

● Ready for Research Use

Each ELISA kit contains a plate coated with antibodies and ready-to-use reagents. Simple operation, no special handling is required.

● 10,000+ Published Publication

CUSABIO ELISA kits are trusted by thousands of researchers worldwide and have been cited in many prestigious high-impact journals, such as Nature, Science, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology, etc.


ELISA Kits Catalog

Here is the catalog of all ELISA kits. You can find the right ones for your research, or search them in the search box on the header part.

Mouse Interleukin 1β (IL-1β) ELISA Kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
78 pg/ml-5000 pg/ml
Sensitivity:
19.5 pg/ml
Size:
96T,5×96T,10×96T

CSB-E08054m(1)

Human Interleukin 12,IL-12/P70 ELISA Kit

Sample Types:
serum, plasma, cell culture supernates
Detect Range:
3.9 pg/ml-250 pg/ml
Sensitivity:
0.975 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04599h-IS

Mouse Interleukin 17,IL-17 ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
10.938 pg/ml-700 pg/ml
Sensitivity:
2.735 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04608m-IS

Human Interleukin 1β,IL-1β ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
2.344 pg/ml-150 pg/ml
Sensitivity:
0.586 pg/ml
Size:
96T,5×96T,10×96T

CSB-E08053h-IS

Mouse Interleukin 2,IL-2 ELISA kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
3.9 pg/ml-250 pg/ml
Sensitivity:
0.975 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04627m-IS

Mouse Interleukin 1β,IL-1β ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
12.5 pg/ml-800 pg/ml
Sensitivity:
3.125 pg/ml
Size:
96T,5×96T,10×96T

CSB-E08054m-IS

Human Interleukin 10,IL-10 ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
3.9 pg/ml-250 pg/ml
Sensitivity:
0.975 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04593h-IS

Mouse Interleukin 6,IL-6 ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
3.9 pg/ml-250 pg/ml
Sensitivity:
0.975 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04639m-IS

Mouse Tumor necrosis factor α,TNF-α ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
7.8 pg/ml-500 pg/ml
Sensitivity:
1.95 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04741m-IS

Human Interferon γ (IFN-γ) ELISA Kit

Sample Types:
serum, plasma, tissue homogenates, cell culture supernates
Detect Range:
7.8 pg/ml-500 pg/ml
Sensitivity:
1.95 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04577h-IS

Mouse Interferon γ,IFN-γ ELISA Kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
0.156 ng/mL-10 ng/mL
Sensitivity:
0.039 ng/mL
Size:
96T,5×96T,10×96T

CSB-E04578m(1)

Mouse C-Reactive Protein,CRP ELISA Kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
5 ng/mL-4000 ng/mL
Sensitivity:
5 ng/mL
Size:
96T,5×96T,10×96T

CSB-E07923m(1)

Mouse Tumor necrosis factor α,TNF-α ELISA KIT

Sample Types:
cell culture supernates, tissue homogenates
Detect Range:
62.5 pg/mL-4000 pg/mL
Sensitivity:
15.6 pg/mL
Size:
96T,5×96T,10×96T

CSB-E04741m(1)

Rat fibroblast growth factor 23 (FGF23) ELISA Kit

Sample Types:
serum, plasma, cell culture supernates, tissue homogenates
Detect Range:
15.6 ng/mL-1000 ng/mL
Sensitivity:
3.9 ng/mL
Size:
96T,5×96T,10×96T

CSB-E12170r(1)

Human Interferon γ (IFN-γ) ELISA Kit

Sample Types:
Cell Culture Supernates
Detect Range:
0.312 ng/ml-20 ng/ml
Sensitivity:
0.078 ng/ml
Size:
96T,5×96T,10×96T

CSB-E04577h

Human Interleukin 12,IL-12/P70 ELISA KIT

Sample Types:
serum, plasma, cell culture supernates
Detect Range:
31.25 pg/ml - 2000pg/ml
Sensitivity:
7.8 pg/ml
Size:
96T,5×96T,10×96T

CSB-E04599h

SARS-CoV-2 N ELISA Kit

Sample Types:
serum, plasma, swabs
Detect Range:
3.12 ng/mL-200 ng/mL
Sensitivity:
0.78 ng/mL
Size:
96T,5×96T,10×96T

CSB-EL33251

SARS-CoV-2 S1 RBD ELISA Kit

Sample Types:
serum, plasma, swabs
Detect Range:
1.25 ng/mL-80 ng/mL
Sensitivity:
0.31 ng/mL
Size:
96T,5×96T,10×96T

CSB-EL33244

SARS-CoV-2 Neutralizing Antibody ELISA Kit

Sample Types:
serum, plasma
Size:
96T,5×96T,10×96T

CSB-EL33243HU

Human SARS-CoV-2 S1 RBD IgM Antibody ELISA Kit

Sample Types:
serum, plasma
Size:
96T,5×96T,10×96T

CSB-EL33242HU

Human SARS-CoV-2 N/S1 IgG Antibody ELISA Kit

Sample Types:
serum, plasma
Size:
96T,5×96T,10×96T

CSB-EL3326HU

Human SARS-CoV-2 S1 RBD IgG Antibody ELISA Kit

Sample Types:
serum, plasma
Detect Range:
Size:
96T,5×96T,10×96T

CSB-EL33241HU

Human SARS-CoV-2 N IgG Antibody ELISA Kit

Sample Types:
serum, plasma
Size:
96T,5×96T,10×96T

CSB-EL3325HU

Mouse low density lipoprotein receptor,LDLR ELISA Kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
0.312 ng/mL-20 ng/mL
Sensitivity:
0.078 ng/mL
Size:
96T,5×96T,10×96T

CSB-EL012846MO

Human neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

Sample Types:
serum, plasma, urine, tissue homogenates
Detect Range:
15.6 pg/mL-1000 pg/mL
Sensitivity:
3.9 pg/mL
Size:
96T

CSB-E09408h

Human Interleukin 18,IL-18 ELISA KIT

Sample Types:
serum, plasma, urine, tissue homogenates
Detect Range:
31.25 pg/mL-2000 pg/mL
Sensitivity:
7.8 pg/mL
Size:
96T,5×96T,10×96T

CSB-E07450h

Rat rotavirus (RV) antigen (Ag) ELISA kit

Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027718RA

Human alpha II-spectrin breakdown product SBDP145 ELISA kit

Sample Types:
serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
Detect Range:
0.625 ng/mL-40 ng/mL
Sensitivity:
0.156 ng/mL
Size:
96T,5×96T,10×96T

CSB-EQ028022HU

Human heparin (Hep) antibody (IgG) ELISA kit

Sample Types:
serum, plasma
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027890HU

Pig brucella antibody (IgG) ELISA kit

Sample Types:
serum, plasma
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027847PI

Plant arachidonic Acid (AA) ELISA kit

Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027590PL

Guinea pig anti-hepatitis B virus surface antibody(HBsAb) ELISA kit

Sample Types:
serum, plasma
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027657GU

Bovine arachidonic Acid(AA) ELISA kit

Sample Types:
serum, plasma,tissue homogenates
Detect Range:
39 ng/mL-2500 ng/mL
Sensitivity:
39 ng/mL
Size:
96T,5×96T,10×96T

CSB-EQ027590BO

Mouse chemokine (C-X-C motif) ligand 17 (CXCL17) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
37.5 pg/mL-2400 pg/mL
Sensitivity:
9.375 pg/mL
Size:
96T,5×96T,10×96T

CSB-EL006246MO

Goat vitamin B12 (VB12) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
1.56 pg/mL-100 pg/mL
Sensitivity:
0.39 pg/mL
Size:
96T,5×96T,10×96T

CSB-EQ028114GO

Horse insulin-like growth factor 1 (IGF1) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
12.5 ng/mL-800 ng/mL
Sensitivity:
3.12 ng/mL
Size:
96T,5×96T,10×96T

CSB-EL011086HO

CSB-EQ028079HU

Human papillomavirus type 18 (HPV18) antibody (IgM) ELISA kit

Sample Types:
serum, plasma
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ028067HU

Mouse complement fragment 3b (C3b) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
0.235 ng/mL-15 ng/mL
Sensitivity:
0.059 ng/mL
Size:
96T,5×96T,10×96T

CSB-EQ027757MO

Human midregional pro-atrial natriuretic peptide (MR-proANP) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
39 pmol/L-2500 pmol/L
Sensitivity:
9.8 pmol/L
Size:
96T,5×96T,10×96T

CSB-EQ028056HU

Rat Lysozyme C(LYZ) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
0.156 ng/mL-10 ng/mL
Sensitivity:
0.039 ng/mL
Size:
96T,5×96T,10×96T

CSB-EL013283RA

Human enterovirus 71 virus (EV71) antibody (IgG) ELISA kit

Sample Types:
serum
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027974HU

Mouse interferon alpha-2(IFNA2) ELISA kit

Sample Types:
serum, plasma, tissue homogenates, cell lysates
Detect Range:
9.38 pg/mL-600 pg/mL
Sensitivity:
2.35 pg/mL
Size:
96T,5×96T,10×96T

CSB-EL011038MO

Rat fibroblast growth factor 21 (FGF21) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
23.5 pg/mL-1500 pg/mL
Sensitivity:
5.8 pg/mL
Size:
96T,5×96T,10×96T

CSB-EL008627RA

Human keyhole limpet hemocyanin antibody(IgE)ELISA kit

Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ028014HU

Pig Progonadoliberin-2(GNRH2) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
40 pg/mL-8000 pg/mL
Sensitivity:
20 pg/mL
Size:
96T,5×96T,10×96T

CSB-EL009636PI

Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
23.5 pg/mL-1500 pg/mL
Sensitivity:
5.86 pg/mL
Size:
96T,5×96T,10×96T

CSB-EL018425MO

Rabbit arachidonic Acid(AA) ELISA kit

Sample Types:
serum, plasma, tissue homogenates
Detect Range:
78.125 ng/mL-5000 ng/mL
Sensitivity:
69.748 ng/mL
Size:
96T,5×96T,10×96T

CSB-EQ027590RB

Human transforming growth factor, beta 2 (TGFB2) autoantibody ELISA kit

Sample Types:
serum, plasma
Detect Range:
Request Information
Sensitivity:
Request Information
Size:
96T,5×96T,10×96T

CSB-EQ027984HU




Customer Reviews

Write Product Reviews
Average Rating:
4.9 - 181 reviews

Review: The sample was heparin anticoagulant plasma, diluted 1:3 for testing, and the result was OK.

Review: I used CSB-E08021h to measure the telomerase content in the lytic fluid of cancer cells, diluted twice, and OD was about 0.3. The scale was normal and the telomerase quantification was consistent with the literature reports. Easy to use, if possible, make the box smaller, better preservation.

Review: The linear correlation of calibration curve is good > 0.9999, and the sample concentration is located in the second half of calibration curve.

Review: I used CSBE08421m to detect the ctnI value of mice with heart failure after myocardial infarction, and the effect of the product was stable.

Review: I used CSBE08421m to detect the ctnI value of mice with heart failure after myocardial infarction, and the effect of the product was stable.

Review: The calibration is accurate, and the sample values are within the calibration range, which is in line with the expected results.


Citations

Mouse Lipopolysaccharides(LPS) ELISA Kit cited in "Hepatic pIgR-mediated secretion of IgA limits bacterial translocation and prevents ethanol-induced liver disease in mice". Gut, 2023

Fish adrenocorticotropic hormone (ACTH) ELISA kit cited in "Acute stress response on Atlantic salmon: a time-course study of the effects on plasma metabolites, mucus cortisol levels, and head kidney transcriptome profile". Fish physiology and biochemistry, 2023

Human Interleukin 6,IL-6 ELISA KIT cited in "Novel Siprulina platensis Bilosomes for Combating UVB Induced Skin Damage". Pharmaceuticals, 2023

Human irisin ELISA kit cited in "Relationship among Body Composition, Adipocytokines, and Irisin on Exercise Capacity and Quality of Life in COPD: A Pilot Study". Biomolecules, 2023

Rat cyclooxygenase-2,COX-2 ELISA Kit cited in "Cyclooxygenase-2 activates EGFR–ERK1/2 pathway via PGE2-mediated ADAM-17 signaling in testosterone-induced benign prostatic hyperplasia". Inflammopharmacology, 2023

Read More >>


Request Trial Samples

24T trial size is available for all ELISA kits except food safety ELISA kits.

Only one 24T trial size kit can be applied for each product for a single customer. No quantity limit for products you can apply.

How to apply for 24T trial size:

StepⅠ: Search for the ELISA kit you need on our website.

StepⅡ: Apply for 24T trial size at the product page.

Explore More >>
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