CLDN6

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC₅₀ is 1.243-2.426 ng/mL.

Activity
Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length Protein with an affinity constant of 5.43 nM as detected by MetaSPR Assay(in presence of DDM/CHS) (WeSPR^TM 200).

CSB-MP005508HU2 is detected by Mouse anti-6*His monoclonal antibody

The purity of VLPs was greater than 95% as determined by SEC-HPLC

The Binding Activity of Huamn CLDN6 with Anti-CLDN6/9 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC₅₀ is 1.501-2.035 ng/mL.

The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC₅₀ is 24.93-35.47 ng/mL.

Untransfected HEK293T cells surface (green line) and transfected Human CLDN6 HEK293T stable cells surface (red line) were stained with anti-CLDN6/9 recombinant antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected MC38 cells (green line) and transfected Human CLDN9 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected CHO-K1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The Binding Activity of Macaca fascicularis CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN6(CSB-MP6148GKN) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 4.186-6.635 ng/mL.
The VLPs (CSB-MP3838) is negative control.

The Binding Activity of Human CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A5)-D) at 2 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 1.243-2.426 ng/mL.

The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity：Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU-D) at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 2.812-3.524 ng/mL.

The Binding Activity of Mouse cldn6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse cldn6(CSB-MP3347MO) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 4.027-6.270 ng/mL.

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005508LA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA005508LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).