CLDN6 is located on chromosome 16p3.3 and has a molecular weight of about 20-40 kDa. The claudins have similar structures to each other, having four transmembrane structural domains, two extracellular loops, and two intracellular tail regions. CLDN6 possesses a C-terminal PDZ binding site allows tight junction proteins to interact with certain intracellular proteins, which is essential in cell junctions and epithelial tissue permeability
More and more studies have shown that abnormal expression of CLDN6 can participate in the occurrence and development of breast cancer, ovarian cancer, cervical cancer, gastric cancer, lung cancer, and other malignant tumors. CLDN6, as an important molecule in the formation of the cytoskeleton and the regulation of cell signaling pathways, can affect the growth process of cells, which provides a new direction for the diagnosis and intervention of tumors. At present, a variety of drugs targeting CLDN6 are in the clinical and preclinical stages and are used for the treatment of many tumors.
Recombinant Human Claudin-6 (CLDN6)-VLPs (Active) (CSB-MP005508HU(A4))
Validated Data
CSB-MP005508HU(A4) is detected by Mouse anti-6*His monoclonal antibody.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 1.501-2.035 ng/mL
The presence of VLP-like structures was confirmed by TEM
Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length VLP Protein with an affinity constant of 6.58 nM as detected by MetaSPR Assay (WeSPRTM 200).
The purity of VLPs was greater than 95% as determined by SEC-HPLC
CLDN6/9 Monoclonal Antibody (CSB-RA005508MA1HU)
Validated Data
The Binding Activity of Huamn CLDN6 with Anti-CLDN6/9 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC50 is 1.501-2.035 ng/mL.
The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU) at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody, the EC50 is 24.93-35.47 ng/mL.
Untransfected HEK293T cells surface (green line) and transfected Human CLDN6 HEK293T stable cells surface (red line) were stained with anti-CLDN6/9 recombinant antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected MC38 cells (green line) and transfected Human CLDN9 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected CHO-K1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
The Binding Activity of Macaca fascicularis CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN6(CSB-MP6148GKN) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC50 is 4.186-6.635 ng/mL.
The VLPs (CSB-MP3838) is negative control.
The Binding Activity of Human CLDN6 with Anti-CLDN6/9 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A5)-D) at 2 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC50 is 1.243-2.426 ng/mL.
The Binding Activity of Human CLDN9 with Anti-CLDN6/9 Recombinant Antibody
Activity:Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 (CSB-MP005511HU-D) at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody. The EC50 is 2.812-3.524 ng/mL.
CLDN6 Recombinant Monoclonal Antibody (CSB-RA005508MA2HU)
Validated Data
The Binding Activity of Human CLDN6 with Anti-CLDN6 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6 recombinant antibody. The EC50 is 2.033-2.415 ng/mL. The VLPs (CSB-MP3838) is negative control.
Untransfected HEK-293T cells (green line) and transfected Human CLDN6 HEK-293T stable cells (red line) were stained with anti-CLDN6 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
The following CLDN6 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CLDN6 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA207788 | Phospho-CLDN6 (Tyr219) Antibody | Human,Mouse | ELISA,WB |
CSB-PA263732 | CLDN6 Antibody | Human | ELISA,WB,IHC,IF |
CSB-PA001673 | CLDN6 Antibody | Human | WB, IHC, IF, ELISA |
CSB-PA070220 | Phospho-CLDN6 (Y219) Antibody | Human,Mouse | WB, IHC, ELISA |
CSB-PA070221 | CLDN6 Antibody | Human,Mouse | IHC, ELISA |
CSB-PA005508LA01HU | CLDN6 Antibody | Human | ELISA, IHC, IF |
CSB-PA005508LB01HU | CLDN6 Antibody, HRP conjugated | Human | ELISA |
CSB-RA005508MA1HU | CLDN6/9 Monoclonal Antibody | Human, Macaca fascicularis | ELISA, FC |
CLDN6 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF005508HU | Recombinant Human Claudin-6 (CLDN6), partial | in vitro E.coli expression system |
CSB-YP005508HU1 CSB-EP005508HU1 CSB-BP005508HU1 CSB-MP005508HU1 CSB-EP005508HU1-B |
Recombinant Human Claudin-6 (CLDN6), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-MP005508HU(A4) | Recombinant Human Claudin-6 (CLDN6)-VLPs (Active) | Mammalian cell |
CSB-MP005508HU(A4)f4 | Recombinant Human Claudin-6 (CLDN6)-VLPs, Fluorescent (Active) | Mammalian cell |
CSB-MP005508HU(A5)-D | Recombinant Human Claudin-6(CLDN6)-Detergent (Active) | Mammalian cell |