FCGR3A

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A at 2 μg/ml can bind Anti-FCGR3A recombinant antibody (CSB-RA008543MA1HU). The EC₅₀ is 0.2330-0.3147 ng/mL.

The purity of FCGR3A was greater than 90% as determined by SEC-HPLC

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-FCGR3A antibody (CSB-RA008543MA2HU) at 2 μg/ml can bind Biotinylated Cynomolgus FCGR3A. The EC₅₀ is 14.09-25.58 ng/ml.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-FCGR3A antibody (CSB-RA008543MA2HU) at 2 μg/ml can bind Biotinylated Human FCGR3A. The EC₅₀ is 7.814-12.17 ng/ml.

The purity of FCGR3A was greater than 95% as determined by SEC-HPLC

Immunofluorescence staining of Hela cell with CSB-RA008543MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The Binding Activity of Rat Gcgr with Anti-Gcgr Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat Gcgr (CSB-MP009316RA1) at 2 μg/mL can bind Anti-Gcgr recombinant antibody. The EC₅₀ is 29.63-35.34 ng/m

The Binding Activity of Human FCGR3A with Anti-FCGR3A Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A (CSB-MP008543HU1) at 2 μg/mL can bind Anti-FCGR3A recombinant antibody. The EC₅₀ is 0.2330-0.3147 ng/mL.