FCGR3A encodes the Fc gamma receptor IIIa, also known as CD16a. This protein is a low - affinity receptor for the Fc region of immunoglobulin G. It plays a crucial role in immune responses, mainly by binding to immune complexes and triggering cell - mediated cytotoxicity through the activation of signaling cascades such as the Syk - PLCγ pathway.
Alterations in FCGR3A function are associated with various diseases, including autoimmune disorders and some types of cancers. In drug development, monoclonal antibodies targeting FCGR3A are being investigated to enhance immune - mediated tumor killing, showing potential in improving cancer immunotherapy.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Loaded Anti-Human CD16a Antibody (CSB-RA008543MA1HU) on 96-Flat plate, can bind Human CD16a(F176V), with an affinity constant of 12 nM as determined in BLI assay (Gator Prime).
FCGR3A Recombinant Monoclonal Antibody (CSB-RA008543MA1HU)
Validated Data
Immunofluorescence staining of Hela cell with CSB-RA008543MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Binding Activity of Rat Gcgr with Anti-Gcgr Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat Gcgr (CSB-MP009316RA1) at 2 μg/mL can bind Anti-Gcgr recombinant antibody. The EC50 is 29.63-35.34 ng/m
The Binding Activity of Human FCGR3A with Anti-FCGR3A Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human FCGR3A (CSB-MP008543HU1) at 2 μg/mL can bind Anti-FCGR3A recombinant antibody. The EC50 is 0.2330-0.3147 ng/mL.
Human Low affinity immunoglobulin gamma Fc region receptor III-A(FCGR3A) ELISA kit (CSB-EL008543HU)
Validated Data
Code: CSB-EL008543HU
Size: 96T,5×96T,10×96T
Sensitivity: 11.75 pg/mL
Detection Range: 47 pg/mL-3000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following FCGR3A reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
FCGR3A Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA287660 | FCGR3A Antibody | Human | ELISA,IHC |
CSB-PA183080 | FCGR3A Antibody | Human | ELISA,IHC |
CSB-PA008543LA01HU | FCGR3A Antibody | Human | ELISA, IHC, IF |
CSB-PA008543LB01HU | FCGR3A Antibody, HRP conjugated | Human | ELISA |
CSB-PA008543LC01HU | FCGR3A Antibody, FITC conjugated | Human | |
CSB-PA008543LD01HU | FCGR3A Antibody, Biotin conjugated | Human | ELISA |
CSB-RA008543MA1HU | FCGR3A Recombinant Monoclonal Antibody | Human, Rat | ELISA, IF |
FCGR3A Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP303590BO CSB-EP303590BO CSB-BP303590BO CSB-MP303590BO CSB-EP303590BO-B |
Recombinant Bovine Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FCGR3A Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP388701MOW CSB-EP388701MOW CSB-BP388701MOW CSB-MP388701MOW CSB-EP388701MOW-B |
Recombinant Macaca mulatta Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FCGR3A Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP636747PI CSB-EP636747PI CSB-BP636747PI CSB-MP636747PI CSB-EP636747PI-B |
Recombinant Pig Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FCGR3A Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP854680MOV CSB-EP854680MOV CSB-BP854680MOV CSB-MP854680MOV CSB-EP854680MOV-B |
Recombinant Macaca fascicularis Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FCGR3A Proteins for Homo sapiens (Human)
FCGR3A ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL008543HU | Human Low affinity immunoglobulin gamma Fc region receptor III-A(FCGR3A) ELISA kit | serum, plasma, tissue homogenates | 11.75 pg/mL |