SRC, sarcoma virus protein Src, alias c-Src, p60-Src, etc. It is a non-receptor tyrosine kinase that is a member of the Src family of proteins encoded by the human cell itself. It is a non-receptor tyrosine kinase, a member of the Src family of protein tyrosine kinases, and is encoded by the SRC gene in human cells themselves.SRC is expressed in a wide range of cell types, and is particularly highly expressed in lymphocytes and osteoblasts.SRC is involved in the regulation of intracellular signaling through its kinase activity, which is capable of phosphorylating the tyrosine residues of a wide range of proteins, thereby activating or inhibiting the function of these proteins.SRC activity is regulated by a number of factors, including phosphorylation and inhibition of these proteins. SRC activity is regulated by a variety of factors, including phosphorylation, dephosphorylation, oligomerization, and binding to membranes, etc.
SRC has a wide range of biological significance, and it is involved in a variety of physiological processes, including cell proliferation, differentiation, migration, adhesion, and apoptosis, etc. It is also known for its ability to activate a variety of downstream signaling pathways, such as MAPK, PI3K/AKT, JAK/STSTM, AKT, and JAK/STM, as well as its ability to activate the cell membrane. AKT, JAK/STAT, etc., SRC plays an important role in cell survival and function. In addition, the abnormal function of SRC is associated with the development of many diseases, especially closely related to tumorigenesis, development and metastasis. In tumor cells, overactivation of SRC can promote tumor cell proliferation and invasion, making it a potential target in tumor therapy. Therefore, small molecule inhibitors against SRC are being developed for the treatment of certain types of cancer.
Recombinant Human Proto-oncogene tyrosine-protein kinase Src (SRC) (CSB-EP022650HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
SRC Recombinant Monoclonal Antibody (CSB-RA022650A0HU)
Validated Data
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate
All lanes: SRC antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 61 KDa
Observed band size: 60, 61 KDa
Immunofluorescence staining of MCF-7 cells with CSB-RA022650A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing SH-SY5Y cells stained with CSB-RA022650A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Human Proto-oncogene tyrosine-protein kinase Src(SRC) ELISA kit (CSB-EL022650HU)
Validated Data
Code: CSB-EL022650HU
Size: 96T,5×96T,10×96T
Sensitivity: 6.25 pg/mL
Detection Range: 25 pg/mL-1600 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following SRC reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
SRC Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA022650GA01HU | SRC Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IP,IF |
CSB-PA954497 | Phospho-SRC (Tyr529) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA829908 | SRC (Ab-529) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA014991 | Phospho-SRC (Tyr418) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA928286 | Phospho-SRC (Ser75) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA000506 | Phospho-SRC (Y529) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA000637 | Phospho-SRC (Y419) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA001803 | SRC Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA001806 | SRC Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA001807 | SRC Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA001809 | SRC Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA050132 | Phospho-SRC (Y216) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA050133 | Phospho-SRC (S75) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA050134 | SRC Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA081645 | SRC Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA080378 | SRC Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA022650XA01HU | SRC Antibody | Homo sapiens (Human) | ELISA, WB (ensure identification of antigen) |
CSB-RA022650A0HU | SRC Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB, IF, FC |
CSB-PA022650ZA01HU | SRC Antibody | Homo sapiens | ELISA, WB (ensure identification of antigen) |
CSB-PA022650LA01HU | SRC Antibody | Human | ELISA, WB, IHC |
CSB-PA022650LB01HU | SRC Antibody, HRP conjugated | Human | ELISA |
CSB-PA022650LC01HU | SRC Antibody, FITC conjugated | Human | |
CSB-PA022650LD01HU | SRC Antibody, Biotin conjugated | Human | ELISA |
CSB-PA022650LA11HU | SRC Antibody | Human | ELISA, IHC |
CSB-PA022650LB11HU | SRC Antibody, HRP conjugated | Human | ELISA |
CSB-PA022650LC11HU | SRC Antibody, FITC conjugated | Human | |
CSB-PA022650LD11HU | SRC Antibody, Biotin conjugated | Human | ELISA |
SRC Antibodies for Gallus gallus (Chicken)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA022650XA01CH | SRC Antibody | Gallus gallus (Chicken) | ELISA, WB (ensure identification of antigen) |
SRC Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA193157XA01DIL | src Antibody | Danio rerio (Zebrafish) (Brachydanio rerio) | ELISA, WB (ensure identification of antigen) |
SRC Antibodies for Zebrafish
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA24479A0Rb | src Antibody | Zebrafish | ELISA, WB |
CSB-PA24479B0Rb | src Antibody, HRP conjugated | Zebrafish | ELISA |
CSB-PA24479C0Rb | src Antibody, FITC conjugated | Zebrafish | |
CSB-PA24479D0Rb | src Antibody, Biotin conjugated | Zebrafish | ELISA |
SRC Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP022650HU CSB-BP022650HU CSB-MP022650HU CSB-EP022650HU-B |
Recombinant Human Proto-oncogene tyrosine-protein kinase Src (SRC) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP022650HU | Recombinant Human Proto-oncogene tyrosine-protein kinase Src (SRC) | E.coli |
CSB-EP022650HUe1 | Recombinant Human Proto-oncogene tyrosine-protein kinase Src (SRC) | E.coli |
SRC Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP022650MO CSB-EP022650MO CSB-BP022650MO CSB-MP022650MO CSB-EP022650MO-B |
Recombinant Mouse Neuronal proto-oncogene tyrosine-protein kinase Src (Src) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SRC Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP022650CH CSB-EP022650CH CSB-BP022650CH CSB-MP022650CH CSB-EP022650CH-B |
Recombinant Chicken Proto-oncogene tyrosine-protein kinase Src (SRC), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SRC Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP894363RA CSB-EP894363RA CSB-BP894363RA CSB-MP894363RA CSB-EP894363RA-B |
Recombinant Rat Proto-oncogene tyrosine-protein kinase Src (Src) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
SRC ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL022650HU | Human Proto-oncogene tyrosine-protein kinase Src(SRC) ELISA kit | serum, plasma, tissue homogenates, cell lysates | 6.25 pg/mL |