Recombinant Human Proto-oncogene tyrosine-protein kinase Src(SRC)

In Stock
Code CSB-EP022650HU
Product Type Recombinant Protein
Size US$2466
Uniprot No. P12931
Lead Time 3-7 business days
Storage Buffer Tris-based buffer,50% glycerol
Species Homo sapiens (Human)
Purity Greater than 85% as determined by SDS-PAGE.
Sequence GSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
Research Area Cancer
Source E.coli
Gene Names SRC
Protein Names Recommended name: Proto-oncogene tyrosine-protein kinase Src EC= 2.7.10.2 Alternative name(s): Proto-oncogene c-Src pp60c-src Short name= p60-Src
Expression Region 2-536aa
Tag Info N-terminal 10xHis-tagged
Mol. Weight 64.2 kDa
Protein Description Full Length of Mature Protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN)
Involvement in disease Thrombocytopenia 6 (THC6)
Subcellular Location Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm, cytoskeleton, Cytoplasm, perinuclear region
Protein Families Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
Tissue Specificity Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.
Database Links

HGNC: 11283

OMIM: 190090

KEGG: hsa:6714

STRING: 9606.ENSP00000350941

UniGene: Hs.195659

Pathway Chemokine signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
VEGF signaling pathway
Focal adhesion
Gap junction
Regulation of actin cytoskeleton
Adherens junction
Rap1 signaling pathway
Endocytosis
Excitatory synapse pathway

Q&A and Customer Reviews

Q&A
Q:

Regarding your protein CSB-EP022650HU, could you please provide some informations?
Have you expressed this protein before, and do you know if the tags can be removed successfully?
Lastly, do you know if any hosts of this protein are expected to produce an active form of the protein?

A:
Very nice to receive your inquiry.
Recombinant Human Proto-oncogene tyrosine-protein kinase Src(SRC)
CSB-EP022650HU >> E.coli
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

GSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFPILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL


Sorry, this is a semi-custom protein, we have never expressed any of these proteins before, neither have tried tag removal.
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
We can try enzyme digestion, but we can't guarantee 100% successfully. The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.
Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity, we can't 100% guarantee its activity.However, as what we provide is the full length protein, and it's purifie under very mild condition, we recommend you to purchase a small size of eukaryotic expressed protein to have a try. Generally, the more advanced expression system is the protein expressed with, the more likely to be active.
Reference: https://www.sciencedirect.com/science/article/pii/007668799100177X
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