Product Details

Uniprot No.

Target Names

Alternative Names

ASV antibody; Avian sarcoma virus antibody; AW259666 antibody; c SRC antibody; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody; cSrc antibody; EC 2.7.10.2 antibody; Neuronal CSRC tyrosine specific protein kinase antibody; Neuronal proto-oncogene tyrosine-protein kinase Src antibody; Neuronal SRC antibody; Oncogene SRC antibody; OTTHUMP00000174476 antibody; OTTHUMP00000174477 antibody; p60 Src antibody; p60-Src antibody; p60c-src antibody; p60Src antibody; pp60c src antibody; pp60c-src antibody; pp60csrc antibody; Proto oncogene tyrosine protein kinase Src antibody; Proto-oncogene c-Src antibody; Proto-oncogene tyrosine-protein kinase Src antibody; Protooncogene SRC antibody; Protooncogene SRC Rous sarcoma antibody; Src antibody; SRC Oncogene antibody; SRC proto oncogene non receptor tyrosine kinase antibody; SRC_HUMAN antibody; SRC1 antibody; Tyrosine kinase pp60c src antibody; Tyrosine protein kinase SRC 1 antibody; Tyrosine protein kinase SRC1 antibody; v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody; V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody; v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human SRC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SRC, also known as proto-oncogene tyrosine-protein kinase Src, serves as a central hub in cellular signal transduction pathways that govern proliferation, survival, adhesion, and migration. As one of the most extensively studied non-receptor tyrosine kinases, SRC plays critical roles in both normal cellular physiology and disease states, with aberrant SRC activity implicated in numerous cancers and its downstream signaling networks representing attractive therapeutic targets.

This recombinant monoclonal antibody, clone 21H5, offers researchers the reproducibility advantages inherent to sequence-defined reagents. Because the antibody is produced from a defined genetic sequence rather than traditional hybridoma methods, you can expect consistent performance across experiments and between lots, eliminating a common source of variability in long-term studies or multi-site collaborations.

The antibody has been validated across multiple detection platforms, providing flexibility for diverse experimental workflows. Western blot analysis demonstrates clear detection of SRC at the expected 60-61 kDa molecular weight in both NIH/3T3 mouse fibroblast and MCF-7 human breast cancer cell lysates, confirming cross-species reactivity between human and mouse samples. Immunofluorescence staining in MCF-7 cells reveals subcellular localization patterns, while flow cytometry validation in SH-SY5Y neuroblastoma cells enables quantitative analysis of SRC expression at the single-cell level.

The affinity-purified rabbit IgG format ensures compatibility with standard secondary detection systems, and the recommended working dilutions span practical ranges for optimization across different sample types and experimental conditions. For researchers investigating oncogenic signaling, cytoskeletal dynamics, or receptor tyrosine kinase pathways, this antibody provides a reliable tool for interrogating SRC biology across multiple experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN) (Probable). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors. Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1. Plays a role in EGF-mediated calcium-activated chloride channel activation. Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor (Probable). Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus. Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling. Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-128'. Phosphorylates CBLC at multiple tyrosine residues, phosphorylation at 'Tyr-341' activates CBLC E3 activity. Involved in anchorage-independent cell growth. Required for podosome formation. Mediates IL6 signaling by activating YAP1-NOTCH pathway to induce inflammation-induced epithelial regeneration.

Gene References into Functions

Mutation in c-Src phosphorylation site of either HK1 or HK2 remarkably abrogates the stimulating effects of c-Src on glycolysis, cell proliferation, migration, invasion, tumorigenesis and metastasis PMID: 28054552
results showed that CAV-1 could promote anchorage-independent growth and anoikis resistance in detached SGC-7901 cells, which was associated with the activation of Src-dependent epidermal growth factor receptor-integrin beta signaling as well as the phosphorylation of PI3K/Akt and MEK/ERK signaling pathways PMID: 30088837
This study shows that Leu33Pro polymorphism of integrin beta 3 modulates platelet Src pY418 and focal adhesion kinase pY397 phosphorylation in response to abnormally high shear stress. Whereas physiological shear stress does not affect platelet signaling, abnormally high-shear stress considerably elevates Src and FAK phosphorylation in both Pro33 and Leu33 platelets. PMID: 29965811
High SRC expression is associated with lung adenocarcinoma. PMID: 30015929
While activation in c-Src is strictly controlled by ATP-binding and phosphorylation, the authors find that activating conformational transitions are spontaneously sampled in Hsp90-dependent Src mutants. PMID: 28290541
High SRC expression is associated with gastric cancer cell migration. PMID: 30015970
Src kinase mediates UV-induced TRPV1 trafficking into cell membrane in HaCaT keratinocytes. PMID: 29080357
Src kinase activation by nitric oxide promotes resistance to anoikis in tumor cell lines. PMID: 29651879
Src and Aurora-A interact upon Golgi ribbon fragmentation; Src phosphorylates Aurora-A at tyrosine 148 and this specific phosphorylation is required for Aurora-A localization at the centrosomes. PMID: 27242098
Study demonstrated that c-Src contributed to hypoxic microenvironment-rendered paclitaxel resistance in human epithelial ovarian cancer cells by G2/M phase arrest deterioration, and through c-Src suppression, FV-429 was capable of reversing the resistance by blocking c-Src/Stat3/HIF-1alpha pathway. PMID: 29324735
Data demonstrated that the Src/Fn14/NF-kappaB axis plays a critical role in NSCLC metastasis. PMID: 29500337
Results suggest that Src promotes EGF-stimulated EMT and migration by upregulation of ZEB1 and ZEB2 through AKT signaling pathway in gastric cancer cells. PMID: 29052277
Combined targeting of AKT and SRC resulted in a synergistic efficacy against human pancreatic cancer growth and metastasis. PMID: 29978609
important roles for c-Src tyrosine kinase in phosphorylation and activation of SLC11A1 in macrophages PMID: 29723216
Our data suggest that targeting Src signaling may be an effective approach to the treatment of ALK-non-small cell lung cancer (NSCLC) with acquired resistance to ALK inhibitors. PMID: 29048652
Src kinase in chemo-naive human primary osteosarcoma cells is differentially activated. PMID: 28786551
This study demonstrates that simultaneous inhibition of c-Met and Src signaling in MD-MSCs triggers apoptosis and reveals vulnerable pathways that could be exploited to develop NF2 therapies. PMID: 28775147
Syntenin mediates SRC function in exosomal cell-to-cell communication. PMID: 29109268
Endothelial cell-derived matrix promotes the metabolic functional maturation of hepatocyte via integrin-Src signaling. PMID: 28470937
The expression of Src under the influence of nilotinib, dasatinib, erlotinib, gefitinib and afatinib was studied in HPV-positive head and neck squamous cell carcinomas. Src expression was significantly increased by all tested tyrosine kinase inhibitors. PMID: 29715092
Multivariate Cox regression analysis suggested that PTPRA expression was an independent prognostic factor in SCC patients. In the cellular models, PTPRA promotes SCC cell proliferation through modulating Src activation as well as cell cycle progression. In conclusion, higher PTPRA level was associated with worse prognosis of SCC patients and PTPRA could promote the cell cycle progression PMID: 28656243
c-Src/MAPK/NF-kB signaling pathway may contribute to the pathogenesis of pre-eclampsia PMID: 28544129
Data indicate the role of tyrosine kinase c-Src (Src) in rescuing Taz (transcriptional coactivator with PDZ-binding motif) from E3 ligase SCF(beta-TrCP)-mediated degradation. PMID: 28154141
Data suggest that response of bronchial epithelial cells to environmental carcinogen benzo[a]pyrene includes activation of AhR/Src/ERK signaling, CYP1A1 induction, and formation of stable DNA adducts. (AhR = aryl hydrocarbon receptor; Src = Src proto-oncogene kinase; ERK = extracellular signal-regulated kinases; CYP1A1 = cytochrome P450 family 1 subfamily A member 1) PMID: 29545172
It is unclear if we may have seen greater clinical activity if we were able to fully inhibit Src in this study, but given the requirement that enrolling patients have documented disease progression on cetuximab, acquired resistant KRAS-mutant clones may have been present, limiting future strategies to reverse EGFR resistance PMID: 28280091
this study shows that simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar PMID: 27181267
mutations in the germline and somatic DNA of the TEK gene were identified and analyze the expression level of Src and phospho-Src (p-Src) in tumor and healthy tissues from patients with facial cutaneo-mucosal venous malformations. PMID: 28316284
SOCS1 antagonizes epithelial-mesenchymal transition by suppressing Src activity, leading to thioredoxin expression and down-regulation of ROS levels in colon cancer cells PMID: 27613835
These findings suggest that the integrin beta4-FAK/Src signaling axis may play a crucial role in clonorchiasis-associated cholangiocarcinoma metastasis during tumor progression. PMID: 28286026
Estrogen receptor-Src signaling plays an important role in ER (+) breast cancer, which shows a high potential for bone metastasis. PMID: 28472954
thrombin binding to PAR-1 receptor activated Gi-protein/c-Src/Pyk2/EGFR/PI3K/Akt/p42/p44 MAPK cascade, which in turn elicited AP-1 activation and ultimately evoked MMP-9 expression and cell migration in SK-N-SH cells. PMID: 27181591
whereas Src activation under shear stress is dominantly ligand-dependent, FAK signaling seems to be mostly shear induced. PMID: 27467982
We provide evidence here that Rab7 is a substrate of Src kinase, and is tyrosine-phosphorylated by Src, withY183 residue of Rab7 being the optimal phosphorylation site for Src. Further investigations demonstrated that the tyrosine phosphorylation of Rab7 depends on the guanine nucleotide binding activity of Rab7 and the activity of Src kinase. PMID: 28336235
Expression of LINC00520 is regulated by oncogenic Src, PIK3CA and STAT3, and may contribute to the molecular etiology of breast cancer. PMID: 27626181
Findings indicate the importance of Src-Stat3 signaling cascade in gallic acid (GA)-mediated tumor-suppression activity and a therapeutic insight of GA for acquired resistance to EGF receptor tyrosine kinase inhibitors in lung cancer. PMID: 27419630
Memo facilitates ER-alpha and c-Src interaction, ER-alpha Y537 phosphorylation, and has the ability to control ER-alpha extra-nuclear localization in breast cancer cells. PMID: 27472465
Data show that MLLT11/AF1q-induced PDGFR signaling enhanced STAT3 activity through Src kinase activation. PMID: 27259262
Loss of myristoylation abolished the tumorigenic potential of Src and its synergy with androgen receptor in mediating tumor invasion. PMID: 29038344
N-WASP positively regulates demarcation membrane system development and proplatelet formation, and the Src family kinases in association with CDC42 regulate proplatelet formation through N-WASP PMID: 27685868
phosphorylation of mATG9 at Tyr8 by Src and at Ser14 by ULK1 functionally cooperate to promote interactions between mATG9 and the AP1/2 complex. PMID: 27934868
Data suggest that myristoylation of Src kinase is essential to facilitate Src-induced and high-fat diet-accelerated prostatic tumor progression; targeting Src kinase myristoylation, which is required for Src kinase association at cellular membrane, blocks dietary fat-accelerated tumorigenesis. PMID: 28939770
elevated levels of cellular Src in serum and phosphorylated Src in primary nasopharyngeal carcinoma tissue correlated with poor outcomes of these patients PMID: 27078847
Results indicate that src-family kinase (Src) is a upstream kinase of T-LAK cell-originated protein kinase (TOPK). PMID: 27016416
We suggest that the induction of SRC results in increased prostate cancer metastasis that is linked to the dysregulation of the AR signaling pathway through the inactivation of miR-203 PMID: 27028864
Data show that afatinib resistant clones were selectively killed by knock down of ERBB3 + c-MET + c-KIT, but not by the individual or doublet knock down combinations, and the combination of afatinib with the SRC family inhibitor dasatinib killed afatinib resistant H1975 cells in a greater than additive fashion. PMID: 26934000
These results suggest that stabilization of delta-catenin by Hakai is dependent on Src. PMID: 28069439
The protein kinase activity of PI3K phosphorylates serine residue 70 on Src to enhance its activity and induce EGFR transactivation following betaAR stimulation. PMID: 27169346
Data show that the solubilising factor UNC119 sequesters myristoylated Src family protein tyrosine kinases (SFKs) to maintain its enrichment at the plasma membrane to enable signal transduction. PMID: 28740133
Data indicate a role for AXL receptor tyrosine kinase (AXL) in regulating the nuclear translocation of epidermal growth factor receptor (EGFR) and suggest that AXL-mediated SRC family kinases (SFKs) and neuregulin-1 (NRG1) expression promote this process. PMID: 28049763
High Src expression is associated with breast cancer. PMID: 28754671

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Involvement in disease

Thrombocytopenia 6 (THC6)

Subcellular Location

Cell membrane; Lipid-anchor. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell junction, focal adhesion.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, SRC subfamily

Tissue Specificity

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Database Links

HGNC: 11283

OMIM: 190090

KEGG: hsa:6714

STRING: 9606.ENSP00000350941

UniGene: Hs.195659

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Code	CSB-RA022650A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate All lanes: SRC antibody at 1.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 61 KDa Observed band size: 60, 61 KDa Immunofluorescence staining of MCF-7 cells with CSB-RA022650A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay histogram showing SH-SY5Y cells stained with CSB-RA022650A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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SRC Recombinant Monoclonal Antibody

Product Details

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Target Background