Ace Antibody

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Code	CSB-PA001154XA01DLU
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Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Ace Polyclonal antibody

Uniprot No.

P07140

Target Names

Ace

Alternative Names

Ace antibody; CG17907Acetylcholinesterase antibody; AChE antibody; EC 3.1.1.7) [Cleaved into: Acetylcholinesterase 16 kDa subunit; Acetylcholinesterase 55 kDa subunit] antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Ace protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Rapidly hydrolyzes choline released into the synapse. It can hydrolyze butyrylthiocholine.

Gene References into Functions

1 engineered disulfide bridge, I327C/D375C increased stability to heat denaturation, urea, & organic solvents & provided protease resistance. The new disulfide bridge links the N-terminal domain (first 356 aa) to the C-terminal domain. PMID: 16686937
Results provide insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge. PMID: 18422651
kinetic studies show that it affects interactions with peripheral ligands and the entrance of some of the bulkier substrates PMID: 18439026
molecular dynamics simulation reveals the insecticidal resistance mechanism of AChE and its mutation PMID: 19263097

Subcellular Location

Cell junction, synapse. Cell membrane; Lipid-anchor, GPI-anchor. Note=Attached to the membrane of the neuronal cholinergic synapses by a GPI-anchor.

Protein Families

Type-B carboxylesterase/lipase family

Database Links

KEGG: dme:Dmel_CG17907

STRING: 7227.FBpp0289713

UniGene: Dm.1179

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