Product Details

Full Product Name

Rabbit anti-Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR106 Polyclonal antibody

Uniprot No.

P04298

Target Names

VACWR106

Alternative Names

VACWR106 antibody; D1RmRNA-capping enzyme catalytic subunit antibody; Virus termination factor large subunit antibody; VTF large subunit antibody; mRNA-capping enzyme 97 kDa subunit antibody; mRNA-capping enzyme D1 subunit antibody; mRNA-capping enzyme large subunit) [Includes: Polynucleotide 5'-triphosphatase antibody; EC 3.1.3.33 antibody; mRNA 5'-triphosphatase antibody; TPase); mRNA guanylyltransferase antibody; EC 2.7.7.50 antibody; GTP--RNA guanylyltransferase antibody; GTase); mRNA antibody; guanine-N(7)-)-methyltransferase antibody; EC 2.1.1.56 antibody; mRNA cap methyltransferase)] antibody

Raised in

Rabbit

Species Reactivity

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Immunogen

Recombinant Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR106 protein

Immunogen Species

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).; The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.

Gene References into Functions

VTF provides the connection between the termination signal in the RNA transcript and viral RNA polymerase to initiate transcription termination. PMID: 26280468
Crystal structure of vaccinia virus mRNA capping enzyme PMID: 24607143
the results presented in this study provide the first in vivo evidence that the vaccinia virus mRNA capping enzyme plays a role in early gene transcription termination and intermediate gene transcription. PMID: 18295814

Subcellular Location

Virion. Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.

Protein Families

DsDNA virus mRNA guanylyltransferase family; Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0 methyltransferase family

Database Links

KEGG: vg:3707562

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VACWR106 Antibody

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