rng Antibody

Code CSB-PA364339XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rng Polyclonal antibody
Uniprot No.
Target Names
rng
Alternative Names
rng antibody; cafA antibody; yhdF antibody; b3247 antibody; JW3216Ribonuclease G antibody; RNase G antibody; EC 3.1.26.- antibody; Cytoplasmic axial filament protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rng protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
An endonuclease that acts in the processing of the 5'-end of precursors of 16S rRNA. It prefers 5'-monophosphorylated over 5'-OH or 5'-triphosphorylated substrates and cleaves single-stranded sites rich in A and U residues; contributes to RNA turnover. 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides for cleavage. The optimal spacing between the 5' end and the scissile phosphate appears to be 6 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated. Processes the 5'-end precursors of 23S rRNA. Participates in processing of tRNA(Pro) (proK and proM). Also involved in metabolism of some mRNAs. Cells overproducing this protein form chains of cell with cytoplasmic axial filaments. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles, or merely regulate the formation of this structure.; Confers adaptive resistance to aminoglycoside antibiotics through modulation of 16S rRNA processing.
Gene References into Functions
  1. Escherichia coli RNase G sensing a 5' monophosphate is required in vivo for the normal rapid decay of functional mRNAs. PMID: 18078441
Subcellular Location
Cytoplasm. Cell inner membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton.
Protein Families
RNase E/G family, RNase G subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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