secG Antibody

Rare Species
Code CSB-PA314852XA01ENV
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes:secG Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 20 kDa
    Observed band size: 20 kDa
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) secG Antibody Polyclonal antibody
Uniprot No.
Target Names
secG Antibody
Alternative Names
Protein-export membrane protein SecG (P12) (Preprotein translocase band 1 subunit) secG b3175 JW3142
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) secG protein (67-110aa)
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG.
Gene References into Functions
  1. Authors show that SecG plays a critical role for the residual export mediated by mutant signal sequences and that the SecYE dimer can often be detected only in the presence of SecG. PMID: 25404704
  2. The hydrophobicity threshold for SecYEG-mediated cell membrane insertion is distinctly lower than that for Sec61-mediated insertion. PMID: 23659793
  3. Data show that ribosome binding is sufficient to open the SecYEG channel. PMID: 23645666
  4. Data indicate taht SecG inversion occurs only when MPIase associates with SecYEG. PMID: 23716687
  5. Charge composition features of model single-span membrane proteins that determine selection of YidC and SecYEG translocase pathways in Escherichia coli PMID: 23355473
  6. analysis of conformational dynamics of the plug domain of the SecYEG protein-conducting channel PMID: 22033919
  7. SecYEG-bound SecA undergoes ATP-dependent conformational changes that are not observed for soluble SecA. PMID: 15850376
  8. cryo-electron microscopy reconstruction of the E. coli protein-conducting channel, SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor PMID: 16292303
  9. cotranslational targeting of a protein to the E. coli SecYEG complex does not ensure its export but that export also depends on a subsequent event that involves sequences both within and just beyond the signal peptide PMID: 19211555

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
SecG family
Database Links
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