Bovine transforming growth factor β1,TGF-β1 ELISA Kit

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Target Data

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Name: Bovine transforming growth factor β1,TGF-β1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14208B

Product Details

Target Name

transforming growth factor, beta 1

Alternative Names

TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit

Abbreviation

TGFB1

Uniprot No.

P18341

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, cell culture supernates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-103
1:2	Average %	88
	Range %	83-97
1:4	Average %	91
	Range %	87-95
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of bovine TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-97
EDTA plasma (n=4)	103	103-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.684	2.636	2.660	2.537
200	2.348	2.454	2.401	2.278
100	1.786	1.884	1.835	1.712
50	1.031	0.997	1.014	0.891
25	0.542	0.542	0.542	0.419
12.5	0.329	0.311	0.320	0.197
6.25	0.214	0.228	0.221	0.098
0	0.119	0.126	0.123

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Data

Function	Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).
Gene References into Functions	TGF-beta1 stimulated lubricin secretion by superficial zone chondrocytes at all densities with twice-a-week TGF-beta treatment. It is noteworthy that the daily treatment of TGF-beta1 increased lubricin much higher compared with twice-a-week treatment. PMID: 28578597 hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells. PMID: 27373199 TGF-beta1 modulates the expression of syndecan-4 in cultured vascular endothelial cells in a biphasic manner. PMID: 28019669 Taken together, Staphylococcus aureus induces TGF-beta1 and bFGF expression through the activation of AP-1 and NF-kappaB in bovine mammary gland fibroblasts. PMID: 26948281 localized to maternal septum in the interdigitation area of cotyledonary villi and caruncle PMID: 26382756 The results identify TGFB1 and ESRRA as likely transcriptional regulators of rumen epithelial development and energy metabolism, respectively, and provide targets for modulation of rumen development and function in the growing calf. PMID: 24767884 the combined treatment with TGF-beta1 and BMP-7 or treatment first with TGF-beta1 followed by BMP-7 was more effective than other treatment groups in both chondrogenic differentiation and SZP secretion. PMID: 23848497 Tenascin-X promotes activation of latent TGF-beta1 and subsequent epithelial to mesenchymal transition in mammary epithelial cells. PMID: 24821840 a detailed computational model for TGF-beta signalling that incorporates elements of previous models together with crosstalking between Smad1/5/8 and Smad2/3 channels through a negative feedback loop dependent on Smad7. PMID: 23804438 Endogenous TGF-beta1 became more bioactive following activation of the transgene protein product in chondrocytes. PMID: 24105960 A novel peptide, P2K, regulating TGF-beta1 signaling had an anabolic effect on bovine intervertebral disc cells and rabbit degenerated discs. PMID: 23124260 Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746 Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease. PMID: 21722622 Immunohistochemistry in rectus abdominis muscle from foetuses at 180 and 260 days post-conception PMID: 12441094 vascular endothelial growth factor indirectly stimulates smooth muscle cell proliferation and migration through the modulation of basic fibroblast growth factor and transforming growth factor beta(1) released by endothelial cells PMID: 12591230 Data show that as antral follicles develop, transforming growth factor (TGF)-beta3 is the most abundant TGF-beta isoform and TGF-beta1 protein levels decline in large follicles. PMID: 14502602 TGF-beta 1 signaling pathway controls pericyte growth state and contractile phenotype PMID: 14609524 Reactive oxygen species mediate TGF-beta1-induced TIMP-3 gene expression PMID: 15203191 MGP plays a role in endothelial cell function, by increasing transforming growth factor-beta1 activity and stimulating VEGF expression PMID: 15456771 Exogenous TGF-beta1, IGF-I, EGF and GH inhibited fetal bovine serum-deficiency-stimulated TGF-beta1 expression in mammary epithelium. PMID: 15747730 ALK5 and Smad4 have roles in TGF-beta1-induced pulmonary endothelial permeability PMID: 16004987 IGF-1 protects against TGF-beta1 mediated apoptosis in mammary gland. PMID: 16077202 TGF-beta1 which is expressed in airways of asthmatics may contribute to irreversible airway remodeling by enhancing airway smooth muscle proliferation PMID: 16390551 The roles of TGF-beta1 and somatotropic pathways proteins in control of the switch between survival and death of bovine mammary epithelial cells are reported. PMID: 17388018 role for TGFbeta signaling in the mechanism of cellular mechanotransduction that is especially significant for joint lubrication PMID: 17968924 Transforming growth factor-beta1 protects against pulmonary artery endothelial cell apoptosis via ALK5. PMID: 18456797 the low friction of articular cartilage can be modified by TGF-beta1 and IL-1beta treatment and that the friction coefficient depends on multiple factors, including superficial zone protein localization and surface roughness PMID: 18683879 Delayed parturition in clone calving may be associated with persistence of elevated TGF-beta-1 expression in late pregnancy. PMID: 19167845 Sustained restoration of circulating latent TGFB1 to levels approaching the normal physiological range does not rescue the infertility phenotype caused by TGFB1 deficiency. PMID: 19383262 TGF-beta1 downregulates caveolin-1 of cultured endothelial cells, involving ALK-5 receptor subtype PMID: 19710365 Show More Hide All
Subcellular Location	Secreted, extracellular space, extracellular matrix
Protein Families	TGF-beta family
Database Links	KEGG: bta:282089 STRING: 9913.ENSBTAP00000027261 UniGene: Bt.469

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Code	CSB-E14208B
Size	96T,5×96T,10×96T
Trial Size
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