Human CXC-chemokine receptor 4(CXCR4)ELISA Kit

Code CSB-E12825h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-X-C motif) receptor 4
Alternative Names C-X-C chemokine receptor type 4 ELISA Kit; CD184 ELISA Kit; CD184 antigen ELISA Kit; Chemokine (C X C motif) receptor 4 ELISA Kit; Chemokine CXC Motif Receptor 4 ELISA Kit; CXC-R4 ELISA Kit; CXCR-4 ELISA Kit; CXCR4 ELISA Kit; CXCR4_HUMAN ELISA Kit; D2S201E ELISA Kit; FB22 ELISA Kit; Fusin ELISA Kit; HM89 ELISA Kit; HSY3RR ELISA Kit; LAP 3 ELISA Kit; LAP3 ELISA Kit; LCR1 ELISA Kit; LESTR ELISA Kit; Leukocyte derived seven transmembrane domain receptor ELISA Kit; Leukocyte-derived seven transmembrane domain receptor ELISA Kit; Lipopolysaccharide associated protein 3 ELISA Kit; Neuropeptide Y receptor Y3 ELISA Kit; NPY3R ELISA Kit; NPYR ELISA Kit; NPYRL ELISA Kit; NPYY3 ELISA Kit; NPYY3R ELISA Kit; Probable G protein coupled receptor lcr1 homolog ELISA Kit; SDF 1 receptor ELISA Kit; SDF-1 receptor ELISA Kit; SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR ELISA Kit; Stromal cell derived factor 1 receptor ELISA Kit; Stromal cell-derived factor 1 receptor ELISA Kit; WHIM ELISA Kit; WHIMS ELISA Kit
Abbreviation CXCR4
Uniprot No. P61073
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human CXCR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-103  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 97  
Range % 90-102  
1:8 Average % 90  
Range % 85-93  
The recovery of human CXCR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-103  
EDTA plasma (n=4) 95 91-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.575 2.555 2.565 2.437  
500 1.928 2.074 2.001 1.873  
250 1.290 1.189 1.240 1.112  
125 0.612 0.603 0.608 0.480  
62.5 0.394 0.382 0.388 0.260  
31.2 0.256 0.245 0.251 0.123  
15.6 0.201 0.199 0.200 0.072  
0 0.133 0.123 0.128    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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May I know are the antibodies used in this ELISA kit polyclonal antibodies or monoclonal antibodies?
Human CXC-chemokine receptor 4(CXCR4)ELISA Kit

Thanks for your inquiry. Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.

Target Background

(From Uniprot)
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.
Gene References into Functions
  1. Functional analysis in human breast cancer cells showed that LL-37 induced the internalization of CXCR4 through approaching Glu268, the residue of CXCR4, independent of the binding pocket (Asp171, Asp262, and Glu288) for CXCR4 inhibitor AMD3100, signifying that LL-37 is a distinct agonist of CXCR4. PMID: 30251699
  2. Together, these data suggest that the S18-2 protein induces epithelial to mesenchymal cell transition through the TWIST2/E-cadherin signalling and, consequently, CXCR4-mediated migration of prostate cancer cells. PMID: 29396484
  3. Study identified a variant near the chemokine receptor CXCR4 that was jointly associated with increased risk for progressive supranuclear palsy and Parkinson's disease. In addition, a mouse model of tauopathy, expression of CXCR4 and functionally associated genes was significantly altered in regions of the mouse brain that accumulate neurofibrillary tangles most robustly. PMID: 29636460
  4. The expression of CXCR4 and mTOR were found to be negatively correlated with remission. Kaplan-Meier analysis indicated a significant decrease in the rate of progression-free survival (PFS) and in that of overall survival (OS) in patients positive for CXCR4 and mTOR expression. PMID: 28952842
  5. the CXCL12-CXCR4 axis promotes the migration, invasion, and EMT processes in B-CPAP cells, at least partly, by activating the NF-kappaB signaling pathway. PMID: 29316404
  6. results demonstrate that non-oxidizable HMGB1 induce a sustained cardiac fibroblasts migration despite the redox state of the environment and by altering CXCL12/CXCR4 axis. This affects proper cardiac remodeling after an infarction. PMID: 28716707
  7. CXCR4 is highly abundant in the zona glomerulosa and in aldosterone producing adenomas suggesting a significant role in adrenocortical physiology and further representing a potential target for molecular imaging of aldosterone-producing tissue. PMID: 29279316
  8. High CXCR4 expression is associated with bladder cancer progression. PMID: 30015971
  9. The overexpression of CXCR4 increased sVCAM1, and the sVCAM1 secreted from CXCR4-overexpressing non-small cell lung carcinoma cells recruited and arrested additional osteoclast progenitors to promote osteoclastogenesis. PMID: 30355915
  10. MiR-125b functions as an important downstream mediator upon the activation of CXCL12/CXCR4 axis. PMID: 28176874
  11. Data suggest that CXCL12 and its receptor CXCR4 are critical in maintaining homeostasis, specifically during hematopoiesis. Present clinical trials (especially in hematological tumors) are testing whether adding CXCR4 inhibitors to impair tumor dissemination will increase effectiveness of ongoing anti-cancer treatments. (CXCL12 = C-X-C motif chemokine ligand 12; CXCR4 = C-X-C motif chemokine receptor-4) [REVIEW] PMID: 29288743
  12. hypoxiainduced expression of CXCR4 promoted trophoblast cell migration and invasion via the activation of HIF1alpha, which is crucial during placentation. PMID: 29786753
  13. CXCR4 expression was up-regulated in NSCLC cell lines. Inhibition of CXCR4 may reduce EMT, invasion and migration of NSCLC cells. PMID: 29972256
  14. results suggest that BCP-ALL cells create a leukemic niche that attracts leukemic cells in a CXCR4/CXCL12-independent manner PMID: 28619846
  15. Serum CXCR4 and CXCL12 levels increase significantly in septic neonates and they are valuable marker in diagnosis of neonatal sepsis. Serum concentrations of both chemokines represent promising novel biomarkers for neonatal sepsis. PMID: 28562124
  16. study provides atomistic-level description of the activation dynamics of the C-X-C chemokine receptor type 4 (CXCR4), a class A GPCR and important drug target PMID: 30238101
  17. CXCL12 and CXCR4 polymorphisms may be risk factors for hepatocellular carcinoma (HCC), and they may be potential HCC markers. PMID: 29741398
  18. The results suggested that CXCR4 is a predictor of poor prognosis and may serve as a biomarker of the mesenchymal subtype in patients with Glioblastoma multiforme (GBM). In addition, CXCR4 mediated the mitogenactivated protein kinase signaling pathway, which was identified specifically in patients with mesenchymal GBM. PMID: 29767255
  19. Stromal cell derived factor-1/C-X-C chemokine receptor type 4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3beta/beta-catenin pathways. PMID: 28067275
  20. EGFR Over-expression and Mutations Leading to Biological Characteristics Changes of Human Lung Adenocarcinoma Cells through CXCR4/CXCL12 Signaling Pathway PMID: 30037369
  21. BACH1 may inhibit the progression of colorectal cancer through BACH1/CXCR4 pathway. PMID: 29481800
  22. High CXCR4 expression is associated with differential expression patterns in adenocarcinoma and squamous cell carcinoma of the lung relative to small cell lung cancer. PMID: 30076481
  23. No significant associations were found between mean plasma levels of either CXCL12 or CXCR4 with age, gender, tumor site, tumor size, lymph-node involvement or tumor stage PMID: 29693336
  24. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)alpha and the stromal derived factor (SDF)1/CXC chemokine receptor type 4 (CXCR4) axis are abnormally expressed in Intrauterine adhesions endometrium. PMID: 29568895
  25. Daily oral administration of AMD070 significantly inhibited the lung metastasis of B88SDF1 cells in nude mice. These results indicated that AMD070 could be useful as a novel orally bioavailable inhibitor of oral cancer metastasis PMID: 29749473
  26. These data demonstrated that JWA suppressed the migration/invasion of breast carcinoma cells by downregulating the expression of CXCR4, and suggested that JWA may harbor prognostic and therapeutic potential in patients with breast cancer. PMID: 29658570
  27. These results suggest that SDF1 (e.g. presented on proteoglycans) can rapidly activate integrins in an allosteric manner by binding to site 2 in the absence of CXCR4. The allosteric integrin activation by SDF1 is a novel target for drug discovery PMID: 29301984
  28. High CXCR4 expression is associated with lymph node metastasis in colorectal cancer. PMID: 29719205
  29. This effect can be suppressed by miR-613 through directly downregulating CXCR4. PMID: 29845707
  30. These results suggest a key role for the CXCR4-CXCL12 chemokine axis in breast cancer progression and highlight the prognostic importance of this chemokine axis for breast cancer survival. PMID: 29516917
  31. CXCR4 can induce PI3Kdelta inhibitor resistance in ABC DLBCL. PMID: 29472546
  32. Our results demonstrated greater expression of pRET and CXCR4 in cisplatinresistant neuroblastomas (NBs). Vandetanib significantly inhibited SHSY5YR cell proliferation, colony formation, and invasion, while downregulating pRET and CXCR4 expression PMID: 29436676
  33. disruption of the CXCR4/CXCL12 axis by CXCR4 antagonist AMD3100 blocked the contribution of both cancer and stromal cells to the metastatic cascade in the liver. PMID: 29436696
  34. LncRNA PRNCR1 up-regulates CXCR4 through targeting miR-211-5p, which affects osteogenic differentiation and thus contributing to osteolysis after hip replacement PMID: 29775758
  35. Results demonstrated that miR-1246 inhibited cell invasion and EMT process by targeting CXCR4 and blocking JAK/STAT and PI3K/AKT signal pathways in lung cancer cells. PMID: 29171984
  36. High CXCR4 expression is associated with hepatocellular and cholangiocellular carcinomas in tumor capillaries. PMID: 29282035
  37. Each of the CXCR4-derived peptides exhibited high affinity for GroEL with a binding stoichiometry near seven. It is found that the peptides interact with the paired alpha helices in the apical domain of the chaperonin. Each of the two chaperonin rings is competent for accommodating all the seven CXCR4 peptides bound to GroEL under saturation conditions. ATP alone or combined with GroES promoted the peptide release from... PMID: 29627450
  38. Down-regulation of CXCR4 significantly reduced the cell proliferation, while remarkably increased the cell apoptosis and apoptotic protein expression levels in osteosarcoma cells. PMID: 29734183
  39. Quercetin suppressed breast cancer stem cell proliferation, self-renewal, and invasiveness. It also lowered the expression levels of proteins related to tumorigenesis and cancer progression, such as aldehyde dehydrogenase 1A1, C-X-C chemokine receptor type 4, mucin 1, and epithelial cell adhesion molecules. PMID: 29353288
  40. Icaritin enhances MSC proliferation, chemotaxis to stromal cell-derived factor-1 and osteogenic differentiation through STAT-3 activation, with a consequential up-regulation in the expression and activity of CXCR4. Phosphorylated STAT-3 binds the CXCr4 promoter upregulating its expression. PMID: 29679717
  41. CXCL11 did not significantly alter the (13)C-(1)H-HSQC spectrum of CXCR4. Our findings point towards ubiquitin as a biased agonist of CXCR4 PMID: 28455789
  42. High CXCR4 expression may define a specific subtype of sporadic malignant peripheral nerve sheath tumor with favorable prognosis. PMID: 29020982
  43. Data support the importance of SDF-1 and CXCR4 expression for loco-regional control and overall survival in HNSCC after primary radiochemotherapy. PMID: 29061496
  44. Presence of SST5, CXCR4 and ETA on tumor cells and of SST3, CXCR4 and ETA on microvessels gradually increased from grade II to grade IV tumors. PMID: 29696364
  45. These data revealed that CXCR4 is a novel hepatocellular carcinoma (HCC)vascular marker for vessel sprouting and could serve as a potential therapeutic target and a predictive factor for sorafenib treatment in patients with HCC PMID: 28223275
  46. Hetero-oligomerization of a1B/D-adrenergic receptor with the chemokine (C-X-C motif) receptor 4:atypical chemokine receptor 3 heteromeric complex is required for a1B/Dadrenergic receptor function. PMID: 28862946
  47. CXCR4+ cells were increased in response to DOXO, mainly in human cardiac mesenchymal progenitor cells (CmPC), a subpopulation with regenerative potential. PMID: 28837147
  48. This work demonstrates distinct roles for the SDF-1/CXCR4 or CXCR7 network in human induced pluripotent stem cell-derived ventricular cardiomyocyte specification, maturation and function. PMID: 28711757
  49. implantation of IGF1R(+) human dental pulp mesenchymal stem cells exerted enhanced neuroplasticity via integrating inputs from both CXCR4 and IGF1R signaling pathways. PMID: 27586516
  50. CXCR4 was overexpressed on systemic lupus erythematosus B cells, positively correlating with disease activity and kidney involvement PMID: 27665947

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Involvement in disease WHIM syndrome (WHIMS)
Subcellular Location Cell membrane, Multi-pass membrane protein, Cell junction, Early endosome, Late endosome, Lysosome
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microv
Database Links

HGNC: 2561

OMIM: 162643

KEGG: hsa:7852

STRING: 9606.ENSP00000386884

UniGene: Hs.593413


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