MAPK3 Antibody

Datasheet
Code CSB-PA037845
Size US$299
Uniprot No. P27361
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA037845(MAPK3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Human liver cancer tissue, 231 cells, Primary antibody: CSB-PA037845(MAPK3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Immunogen Synthetic peptide of Human MAPK3
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA,WB,IHC;ELISA:1:1000-1:2000,WB:1:200-1:1000,IHC:1:15-1:50
Relevance The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias mitogen-activated protein kinase 3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names MAPK3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Subcellular Location Cytoplasm, Nucleus, Membrane, caveola
Protein Families Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

HGNC: 6877

OMIM: 601795

KEGG: hsa:5595

STRING: 9606.ENSP00000263025

UniGene: Hs.861

Pathway cAMP signaling pathway
Chemokine signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
HIF-1 signaling pathway
MAPK signaling pathway
Melanogenesis
mTOR signaling pathway
PI3K-Akt signaling pathway
TGF-beta signaling pathway
VEGF signaling pathway
Focal adhesion
Gap junction
Lipids and Inflammation in Atherogenesis
Regulation of actin cytoskeleton
TNF signaling pathway
Vascular smooth muscle contraction
Adherens junction
Autophagy
Cellular senescence
MAPK Erk pathway
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
B cell receptor signaling pathway
Fc epsilon RI signaling pathway
Fc gamma R-mediated phagocytosis
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway
cGMP-PKG signaling pathway
FoxO signaling pathway
Insulin signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway
Rap1 signaling pathway
Sphingolipid signaling pathway
Excitatory synapse pathway
Glutamatergic synapse
Neurotrophin signaling pathway

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