Phospho-CTNNB1 (Ser37) Antibody

Datasheet
Code CSB-PA596316
Product Type Polyclonal Antibody
Former Code(s) CSB-PA546162
Size US$360
Uniprot No. P35222
Image
  • Western blot analysis of extracts from HT29 cells using b-Catenin(Phospho-Ser37) Antibody and the same antibody preincubated with blocking peptide .
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser37) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunogen Peptide sequence around phosphorylation site of serine 37 (I-H-S(p)-G-A) derived from Human b-Catenin.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of b-Catenin only when phosphorylated at serine 37.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Relevance Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias CTNNB1; CATNB; CTNB1; CTNNB;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names CTNNB1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin
Involvement in disease Colorectal cancer (CRC); Pilomatrixoma (PTR); Medulloblastoma (MDB); Ovarian cancer (OC); Mesothelioma, malignant (MESOM); Mental retardation, autosomal dominant 19 (MRD19); Vitreoretinopathy, exudative 7 (EVR7)
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, Cell junction, adherens junction, Cell junction, Cell membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cell junction, synapse
Protein Families Beta-catenin family
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (Pub
Database Links

HGNC: 2514

OMIM: 114500

KEGG: hsa:1499

STRING: 9606.ENSP00000344456

UniGene: Hs.476018

Pathway Hippo signaling pathway
Melanogenesis
Wnt signaling pathway
Focal adhesion
Adherens junction
Signaling pathways regulating pluripotency of stem cells
Leukocyte transendothelial migration
Rap1 signaling pathway

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