Recombinant Bacillus subtilis Transcriptional regulatory protein ComA (comA)

Code CSB-YP318487BRJ
MSDS
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Source Yeast
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Code CSB-EP318487BRJ
MSDS
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Source E.coli
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Code CSB-EP318487BRJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318487BRJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP318487BRJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
comA
Uniprot No.
Alternative Names
comA; comA1; comAA; BSU31680Transcriptional regulatory protein ComA; Competence protein A
Species
Bacillus subtilis (strain 168)
Expression Region
1-214
Target Protein Sequence
MKKILVIDDH PAVMEGTKTI LETDSNLSVD CLSPEPSEQF IKQHDFSSYD LILMDLNLGG EVNGMELSKQ ILQENPHCKI IVYTGYEVED YFEEAIRAGL HGAISKTESK EKITQYIYHV LNGEILVDFA YFKQLMTQQK TKPAPSSQKE QDVLTPRECL ILQEVEKGFT NQEIADALHL SKRSIEYSLT SIFNKLNVGS RTEAVLIAKS DGVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Response regulator in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Regulates directly the expression of over 20 genes, including genes of the srfA operon, degQ, rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the regulation of comK transcription, the expression of late competence genes.
Gene References into Functions
  1. The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. PMID: 26582911
  2. Using NMR spectroscopy, the high-resolution 3D solution structure of the C-terminal DNA-binding domain of ComA (ComAC) has been determined. PMID: 20302877
  3. Data show that purified ComA forms a dimer in solution, and proposed a model for how two dimers of ComA bind to an odd number of DNA-binding determinants to activate transcription of target genes. PMID: 18585392
Subcellular Location
Cytoplasm.
Database Links
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