Recombinant Dog Cellular tumor antigen p53(TP53)

Code CSB-YP643685DO
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Source Yeast
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Code CSB-EP643685DO
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Source E.coli
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Code CSB-EP643685DO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP643685DO
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Source Baculovirus
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Code CSB-MP643685DO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names TP53
Uniprot No. Q29537
Alternative Names TP53; P53; Cellular tumor antigen p53; Tumor suppressor p53
Species Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region 1-381
Target Protein Sequence MEESQSELNI DPPLSQETFS ELWNLLPENN VLSSELCPAV DELLLPESVV NWLDEDSDDA PRMPATSAPT APGPAPSWPL SSSVPSPKTY PGTYGFRLGF LHSGTAKSVT WTYSPLLNKL FCQLAKTCPV QLWVSSPPPP NTCVRAMAIY KKSEFVTEVV RRCPHHERCS DSSDGLAPPQ HLIRVEGNLR AKYLDDRNTF RHSVVVPYEP PEVGSDYTTI HYNYMCNSSC MGGMNRRPIL TIITLEDSSG NVLGRNSFEV RVCACPGRDR RTEEENFHKK GEPCPEPPPG STKRALPPST SSSPPQKKKP LDGEYFTLQI RGRERYEMFR NLNEALELKD AQSGKEPGGS RAHSSHLKAK KGQSTSRHKK LMFKREGLDS D
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Gene References into Functions
  1. These data suggest that the p53 mutant cell line CTBm2 could be a useful tool for analyzing the precise tetramerization mechanisms of p53 and verifying the effects of therapeutic agents against tumors expressing p53 mutants that lack the ability to tetramerize PMID: 29750295
  2. Elevation of the tumour suppressor p53 is necessary and sufficient for crowding hypersensitivity in MDCK cell. PMID: 27109213
  3. TP53 gene expression alone cannot be considered a marker for tumor aggressiveness in canine mammary carcinomas. PMID: 28754073
  4. In all normal samples, p53 was expressed in low number of epithelial cells, while a greater number of positive cells were observed in benign prostatic hyperplasia and prostatic carcinomas. Rb protein was expressed in high number of normal, hyperplastic and neoplastic cells without a statistically significant differences. PMID: 27033932
  5. Serum starvation and TdR methods could achieve sufficient synchronization of CHMm cells. Moreover, the expression of p27, p53 and bcl-2 genes was related to cyclical movements and apoptosis. PMID: 27033900
  6. Mutations of p53 gene were detected in a proportion of canine lymphoma cells from untreated dogs and can be associated with a poor prognosis. PMID: 26678182
  7. p53 knockdown abolished the miR-214-COP1-mediated apoptosis; thus, miR-214 and COP1 regulated apoptosis through controlling p53 in hemangiosarcoma. PMID: 26335793
  8. 14-3-3sigma is deregulated in canine TCCs and its expression by highly infiltrative tumor cells may be related to the acquisition of aggressive behavior. PMID: 25979650
  9. data suggest that upon inactivating mutation of the p53 gene, mutant p53 acquires its gain of function by altering morphogenesis and promoting cell migration and invasion in part by upregulating EMT and c-Met. PMID: 24386484
  10. These results suggest that impaired neurotrophin signaling or compromised trophic support to the retina and p53-mediated apoptosis may not be the underlying mechanism of retinal ganglion cell death in a beagle model of glaucoma. PMID: 22524196
  11. identification of the TP53 gene mRNA as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of canine transmissible venereal tumor samples; findings showed the DNA homologous to TP53 and its respective mRNA in 92.3 percent of the samples PMID: 22077405
  12. case report, Arg-Gln substitution at codon 248 and CHK2 mutations resulting in multiple primary neoplasms and SIRT1 down-regulation PMID: 21890154
  13. Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. PMID: 20447023
  14. Four new polymorphisms of TP53 in canine transmissible venereal tumor samples and their groupings are reported. PMID: 19934603
  15. The clonal origins of the canine transmissible venereal tumor were studied in relation to the T963C mutation on the tumor protein p53. PMID: 17668284
  16. Data show that canine bladder cancer cell lines expressed several proteins of interest associated with bladder cancer prognosis and progression in humans, including p53, cox-2, and pRb protein. PMID: 18562222
  17. Mutations of the TP53 gene may influence survival and should be considered when evaluating canine osteosarcoma. PMID: 18986312
  18. These data support the notion that the decrease of p63 expression, in particular of its isoform DeltaNp63, seems to be an important factor in the development of carcinomas in mixed tumors. PMID: 19176510
  19. Taken together the results suggest that loss of p21 overexpression via its regulator p53 is associated with tumor metastasis while reduced cell cycle inhibition by p27 is associated with malignant progression. PMID: 19185891

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Involvement in disease p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix
Protein Families P53 family
Database Links

KEGG: cfa:403869

STRING: 9615.ENSCAFP00000024579

UniGene: Cfa.3658

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