Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

cea

Uniprot No.

P02978

Research Area

Tags & Cell Markers

Alternative Names

cea; Colicin-E1

Species

Escherichia coli

Source

E.coli

Expression Region

1-304aa

Target Protein Sequence

METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

38.2 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Escherichia coli cea protein is an E.coli-expressed partial protein. Molecularly, this cea protein is characterized by N-terminal 10xHis tag and C-terminal Myc tag, internal Escherichia coli cea DNA fragment (1-304aa). Standard methods for recombinant cea protein expression comprise transfecting cells with DNA vectors that consist of specific templates and then cultures cells to translate and transcribe the cea protein production process. Typically, these cells are lysed to extract expressed proteins for more purification. The purity of this recombinant cea protein is 85%+ measured by SDS-PAGE.

cea, also known as colicin E1, is a bacteriocin produced by E. coli that acts against bacteria by forming a pore in the bacterial membrane, leading to membrane depolarization and cell death. The cobalamin translocator BtuB binds to the colicin E1, initiating the import of colicin E1. The drug-export protein TolC is essential for the import of colicin E1 across the outer membrane and periplasmic space. Treatment of cells harboring the plasmid ColE1 with chemicals that damage DNA or interfere with DNA synthesis can result in the production of colicin E1.

Target Background

Function

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.; Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Channel forming colicin family

Code	CSB-EP360926ENL1
Abbreviation	Recombinant E.coli cea protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea.
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Recombinant Escherichia coli Colicin-E1 (cea), partial

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