Recombinant Escherichia coli Colicin-E1(cea),partial

In Stock
Code CSB-EP360926ENL1
Size US$2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360926ENL1 could indicate that this peptide derived from E.coli-expressed Escherichia coli cea.
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Product Details


Recombinant Escherichia coli cea protein is an E.coli-expressed partial protein. Molecularly, this cea protein is characterized by N-terminal 10xHis tag and C-terminal Myc tag, internal Escherichia coli cea DNA fragment (1-304aa). Standard methods for recombinant cea protein expression comprise transfecting cells with DNA vectors that consist of specific templates and then cultures cells to translate and transcribe the cea protein production process. Typically, these cells are lysed to extract expressed proteins for more purification. The purity of this recombinant cea protein is 85%+ measured by SDS-PAGE.

cea, also known as colicin E1, is a bacteriocin produced by E. coli that acts against bacteria by forming a pore in the bacterial membrane, leading to membrane depolarization and cell death. The cobalamin translocator BtuB binds to the colicin E1, initiating the import of colicin E1. The drug-export protein TolC is essential for the import of colicin E1 across the outer membrane and periplasmic space. Treatment of cells harboring the plasmid ColE1 with chemicals that damage DNA or interfere with DNA synthesis can result in the production of colicin E1.

Purity Greater than 85% as determined by SDS-PAGE.
Target Names cea
Uniprot No. P02978
Research Area Tags & Cell Markers
Alternative Names
cea; Colicin-E1
Species Escherichia coli
Source E.coli
Expression Region 1-304aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 38.2 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Tris-based buffer,50% glycerol
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.; Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families Channel forming colicin family
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