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This Human mTOR ELISA Kit was designed for the quantitative measurement of Human mTOR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.
|Target Name||mammalian target of rapamyein,mTOR|
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) ELISA Kit; FK506 binding protein 12 rapamycin associated protein 1 ELISA Kit; FK506 binding protein 12 rapamycin associated protein 2 ELISA Kit; FK506 binding protein 12 rapamycin complex associated protein 1 ELISA Kit; FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit; FKBP rapamycin associated protein ELISA Kit; FKBP12 rapamycin complex associated protein ELISA Kit; FKBP12-rapamycin complex-associated protein 1 ELISA Kit; FKBP12-rapamycin complex-associated protein ELISA Kit; FLJ44809 ELISA Kit; FRAP ELISA Kit; FRAP1 ELISA Kit; FRAP2 ELISA Kit; Mammalian target of rapamycin ELISA Kit; Mechanistic target of rapamycin ELISA Kit; mTOR ELISA Kit; MTOR_HUMAN ELISA Kit; OTTHUMP00000001983 ELISA Kit; RAFT1 ELISA Kit; Rapamycin and FKBP12 target 1 ELISA Kit; Rapamycin associated protein FRAP2 ELISA Kit; Rapamycin target protein 1 ELISA Kit; Rapamycin target protein ELISA Kit; RAPT1 ELISA Kit; Serine/threonine-protein kinase mTOR ELISA Kit
|Species||Homo sapiens (Human)|
|Sample Types||serum, plasma, tissue homogenates|
|Detection Range||1.56 pg/mL-100 pg/mL|
|Detection Wavelength||450 nm|
|Research Area||Cell Biology
|ELISA Data Analysis||Watch ELISA data processing video & download Curve Expert if needed|
|ELISA kit FAQs|
|Storage||Store at 2-8°C. Please refer to protocol.|
|Lead Time||3-5 working days|
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Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. This also includes mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3: in the presence of nutrients, mediates phosphorylation of TFEB and TFE3, promoting their cytosolic retention and inactivation. Upon starvation or lysosomal stress, inhibition of mTORC1 induces dephosphorylation and nuclear translocation of TFEB and TFE3, promoting their transcription factor activity. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions. Prevents autophagy by mediating phosphorylation of AMBRA1, thereby inhibiting AMBRA1 ability to mediate ubiquitination of ULK1 and interaction between AMBRA1 and PPP2CA. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422'. Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms. Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks. Phosphorylates SQSTM1, promoting interaction between SQSTM1 and KEAP1 and subsequent inactivation of the BCR(KEAP1) complex.
|Gene References into Functions||
|Involvement in disease||Smith-Kingsmore syndrome (SKS); Focal cortical dysplasia 2 (FCORD2)|
|Subcellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm. Nucleus, PML body. Microsome membrane. Lysosome membrane. Cytoplasmic vesicle, phagosome.|
|Protein Families||PI3/PI4-kinase family|
|Tissue Specificity||Expressed in numerous tissues, with highest levels in testis.|