CASP3 Antibody

Datasheet
Code CSB-PA001231
Size US$167
Image
  • Western Blot analysis of KB cells using Caspase-3 Polyclonal Antibody
  • Western Blot analysis of KB MCF7 cells using Caspase-3 Polyclonal Antibody
  • Western blot analysis of Hela KB 293T 3T3 lysis using Caspase-3 antibody.

Product Details

Uniprot No. P42574
Target Names CASP3
Alternative Names CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human Caspase-3 around the non-phosphorylation site of S150.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

The novel thioredoxin reductase inhibitor A-Z2 triggers intrinsic apoptosis and shows efficacy in the treatment of acute myeloid leukemia. Dongdong Zhang, et al,Free Radical Biology and Medicine,2019

Sample type: AML cell lines
Sample species: Mouse
Application: WB
Review: AML cells were treated with 1 µM A-Z2 for 24 h, cell supernatants were harvested, cytochrome c level was measured by ELISA. Apoptosis (F) and cell cycle were analyzed after Molm-13 were treated with 1 µM A-Z2 for 24 h. AML-PC: AML patient primary cell.
PMID: 31730934

Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

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