CASP3 Antibody

Code CSB-PA001231
Size US$167
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  • Western Blot analysis of KB cells using Caspase-3 Polyclonal Antibody
  • Western Blot analysis of KB MCF7 cells using Caspase-3 Polyclonal Antibody
  • Western blot analysis of Hela KB 293T 3T3 lysis using Caspase-3 antibody.
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Product Details

Uniprot No. P42574
Target Names CASP3
Alternative Names A830040C14Rik antibody; Apopain antibody; CASP 3 antibody; CASP-3 antibody; CASP3 antibody; CASP3_HUMAN antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3; apoptosis-related cysteine peptidase antibody; Caspase 3; apoptosis-related cysteine protease antibody; Caspase 3; apoptosis-related cysteine protease a antibody; Caspase-3 subunit p12 antibody; Caspase3 antibody; CC3 antibody; CPP 32 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 3.4.22.56 antibody; ICE3 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SCA1 antibody; SREBP cleavage activity 1 antibody; Yama antibody; Yama protein antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human Caspase-3 around the non-phosphorylation site of S150.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

The novel thioredoxin reductase inhibitor A-Z2 triggers intrinsic apoptosis and shows efficacy in the treatment of acute myeloid leukemia. Dongdong Zhang, et al,Free Radical Biology and Medicine,2019

Sample type: AML cell lines
Sample species: Mouse
Application: WB
Review: AML cells were treated with 1 µM A-Z2 for 24 h, cell supernatants were harvested, cytochrome c level was measured by ELISA. Apoptosis (F) and cell cycle were analyzed after Molm-13 were treated with 1 µM A-Z2 for 24 h. AML-PC: AML patient primary cell.
PMID: 31730934

Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Gene References into Functions
  1. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
  2. As phosphorylation of the serine residue of this tetra-peptide could yield a motif similar to the caspase-3 binding recognition sequence DEVD/E, the region from a representative PE_PGRS protein (PE_PGRS45) was docked to human caspase-3. PMID: 30207307
  3. within the modified loop, Ser(150) evolved with the apoptotic caspases, whereas Thr(152) is a more recent evolutionary event in mammalian caspase-3. Substitutions at Ser(150) result in a pH-dependent decrease in dimer stability, and localized changes in the modified loop propagate to the active site of the same protomer through a connecting surface helix. PMID: 29414778
  4. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
  5. Study seems to indicate a direct connection between SNPS in CASP3 gene and prostate cancer (PCa) risk in the Galician population after stratification. Also, individual susceptibility to PCa becomes more evident when assessing gene-environment interactions. Alleles G and T, in rs1049216 and rs2705897 respectively, are related to an increased risk of PCa in smokers overweight individuals. PMID: 30176316
  6. Low CASP3 expression is associated with Colorectal Cancer. PMID: 29801534
  7. overexpressed miR-337-3p and miR-17-5p/miR-132-3p/-212-3p can regulate executioner caspases-3 and -7, respectively. PMID: 29659498
  8. Caspase-8 and Caspase-3 expressions in tumor tissues are novel candidate prognostic markers for colorectal cancer patients PMID: 29355114
  9. The new findings of this work were that an association between serum caspase-3 concentrations during the first week, apoptosis degree, sepsis severity, and sepsis mortality exists. PMID: 29119350
  10. our data demonstrate that WT1 protein undergoes proteolytic processing by caspase-3 in chemotherapeutic drugs-induced apoptosis. This processing is associated with a reduction of WT1 protein. PMID: 28395566
  11. Increased baseline gene expressions of RUNX2, p21 and caspase 3 in the peripheral blood might predict better responses to methotrexate therapy. PMID: 28741869
  12. The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
  13. a prolonged anti-apoptotic intervention targeting caspase-3 should be considered with caution due to the potential adverse effects in mitochondria dynamics resulting from a novel potential functional role of procaspase-3 in mitochondrial biogenesis via regulating the expression of mitochondrial biogenesis activators. PMID: 28585712
  14. knockdown of RPA1 suppressed cell clone formation, induced cell cycle arrest at the G1 phase and promoted cell apoptosis by regulating the protein level of Caspase 3 PMID: 29601890
  15. The phosphorylation level of p38 was upregulated by MA1 treatment, and the inhibitor of p38, SB203580, attenuated the MA1-induced p38 phosphorylation as well as caspase3 and PARP activation. These results indicate that MA1 treatment alters invasive and oncogenic phenotypes of human colorectal cancer cells through the stimulation of the p38 signaling pathway PMID: 28713983
  16. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
  17. Here the authors show that sublethal activation of Caspase-3 plays an essential, facilitative role in Myc-induced genomic instability and oncogenic transformation. PMID: 28691902
  18. we show that ABT-737 and TQ activate PKA in a caspase-3-dependent manner, which correlates with platelet inhibition and apoptosis and therefore potentially contributes to the bleeding risk in chemotherapy patients PMID: 28661475
  19. MiR-221 might represent a candidate biomarker of likelihood of response to Sorafenib in HCC patients to be tested in future studies. Caspase-3 modulation by miR-221 participates to Sorafenib resistance PMID: 28096271
  20. In the present study, galangin was found to suppress laryngeal cancer cell proliferation. Also, flow cytometry, immunohistochemical and western blot analysis indicated that cell apoptosis was induced for galangin administration, promoting caspase-3 expression through regulating PI3K/AKT/NF-kappaB. PMID: 28677816
  21. 1,4-BQ evidently induced mitochondria-mediated apoptosis and increased pro-apoptotic genes (Caspase-9 and Caspase-3) expression in a dose-dependent manner. PMID: 27425441
  22. GGN played a tumor-promoting role in bladder cancer through regulation of NFkappaB/caspase3-mediated apoptosis signaling. PMID: 29412153
  23. Serum caspase-3 concentrations are increased in ICH patients as well as correlate with clinical severity and prognosis PMID: 28526532
  24. High caspase-3 expression is significantly associated with adverse breast cancer-specific survival. High caspase-3 expression was significantly associated with HER2 positive tumours.The prognostic significance of caspase-3 expression in different breast cancer phenotypes was also examined.There was a significant association in receptor positive (ER, PR or HER2) and non-basal like subgroups. PMID: 27798717
  25. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
  26. Overexpression of CASP3 is associated with Breast Cancer. PMID: 26932709
  27. Results show that CASP3 expression is regulated by HOXC13 which represses its transcription by directly targeting its promotor region. PMID: 29168599
  28. Data show that selective histone deacetylase 6 (HDAC6) inhibition or knockdown of HDAC6 expression was able to prevent caspase 3 activation in lung endothelial cells and maintain lung endothelial cell-cell junctions. PMID: 27419634
  29. Genetic variations in the CASP3 gene and the joint effects of working time and CASP3 polymorphisms may modify the risk of developing noise-induced hearing loss PMID: 28738811
  30. Data indicate that through upregulating the expression of caspase-3, the TT genotype of caspase-3 rs1049216 can be associated with not only the risk of cervical cancer but also the progression of this cancer. PMID: 28114230
  31. In conclusion, our findings firstly revealed that GSDME switches chemotherapy drug-induced caspase-3 dependent apoptosis into pyroptosis in gastric cancer cells. PMID: 29183726
  32. Everolimus also induced higher levels of caspase-3/-7 activation in GR over GS cells, and everolimus-mediated mTOR inhibition lead to G2 arrest in GR cells but G1 arrest in GS cells. PMID: 28165150
  33. Results Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1 supporting an inhibitory role of Grb7 on Casp3 cleavage function by interfering with the association of Casp3 and Hax1. PMID: 26869103
  34. caspase-3 inhibitors also suppressed the attenuation of cell adhesion and phosphorylation of p38 MAPK by EGF-F9. Our data indicated that EGF-F9 activated signals for apoptosis and induced de-adhesion in a caspase-3 dependent manner. PMID: 27129300
  35. Data indicate that E-cadherin and caspase-3 were targets of miR-421, which was up-regulated by HIF-1alpha. PMID: 27016414
  36. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
  37. These results demonstrate that hyperglycemic-induced endothelial microparticles increase endothelial cell active caspase-3. This apoptotic effect may be mediated, at least in part, by a reduction in miR-Let-7a expression. PMID: 28942148
  38. Epigallocatechin-3-Gallate protects against Ang II-induced HUVEC apoptosis by decreasing oxidative stress and ameliorating mitochondrial injury via activation of Nrf2/casp3 signaling pathway. PMID: 28942440
  39. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
  40. cleaved caspase-3 and caspase-3/8/9 could be biomarkers for tumorigenesis in oral tongue squamous cell carcinoma patients. PMID: 28700659
  41. TT genotype of CASP3 rs4643701 polymorphisms showed risk in CAD. CASP3 rs4647601 creates a new exon splicing enhancer. PMID: 28633917
  42. These findings shed some light on how a tumor cell may avert apoptosis using Hsp60 and point to the anti-cancer potential of drugs, such as CubipyOXA, which interfere with Hsp60/pC3 complex formation, and thus allow the apoptotic cascade to proceed. PMID: 28212901
  43. Here the authors show that in macrophages SipA induces increased caspase-3 activation early in infection. PMID: 28630067
  44. SASH1 is cleaved by caspase-3 following Ultraviolet C-induced apoptosis. PMID: 27831555
  45. Caspase 3 activation in dying glioma cells unfavorably supported post-irradiation angiogenesis. PMID: 27826040
  46. CASP3 is a direct target of specific Epstein-Barr virus BART miRNAs. PMID: 27565721
  47. Data suggest that EV71 infection in enterocytes does not inhibit phosphorylation of STAT1/2 induced by IFN-beta, but p-STAT1/2 transport into the nucleus is significantly blocked; EV71 infection in enterocytes down-regulates expression of KPNA1 and induces degradation of cellular KPNA1 via caspase-3. [EV17 = Enterovirus 71] PMID: 28455446
  48. our results identified that mammalian sterile 20-like kinase 1 is a novel downstream target of pyruvate kinase M2, and knockdown of pyruvate kinase M2 contributes apoptosis via promoting nuclear translocation of mammalian sterile 20-like kinase 1 by enhancing Caspase-3-dependent cleavage. PMID: 28656802
  49. High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of coronary events in subjects from the general population. PMID: 28302628
  50. Interestingly, EspC-induced apoptosis was triggered through a dual mechanism involving both independent and dependent functions of its EspC serine protease motif, the direct cleavage of procaspase-3 being dependent on this motif. PMID: 27329750
  51. LRRC8A is an essential regulator of cisplatin induced p53 protein activity and its downstream signaling involving increased expression of p21Waf1/Cip1 and MDM2, as well as activation of caspase-9 and -3 in tumor cell lines. PMID: 26984736
  52. Our results provide evidence that inhibition of the denitrosylation of S-nitrosylated procaspase-3 mediated by the redox protein Trx2 is a part of the microglial pro-tumoral activation pathway initiated by glioma cancer cells. PMID: 27618552
  53. These results highlight apoptosis-related CASP3 as an important gene in human carcinogenesis and further support the CASP3 polymorphisms confer to the lung cancer susceptibility. PMID: 27723786
  54. These findings revealed that rs4647693 G/A, rs4647610 A/G, and rs12108497 T/C polymorphisms of Caspase-3 may be associated with susceptibility to intervertebral disc herniation. PMID: 27847097
  55. Data show that the apoptotic and cytotoxic effects of BL-038 are mediated by the intrinsic mitochondria-mediated apoptotic pathway, which in turn causes the activation of caspase-9 and caspase-3, and the cleavage of poly (ADP-ribose) polymerase (PARP), to elicit apoptosis response. PMID: 27618007
  56. Data show that MeCP2 promotes gastric cancer (GC) cell proliferation via FOXF1-mediated Wnt5a/beta-Catenin signaling pathway, and suppresses GC cell apoptosis through MYOD1-mediated Caspase-3 signaling pathway. PMID: 28131747
  57. Caspase-3 signaling pathway is involved in breast cancer cells apoptosis. PMID: 28075472
  58. Downregulation of DNMT3a regulated miR-182 via caspase-3 and -9-mediated apoptosis and DNA damage response in ovarian cancer. PMID: 27748882
  59. High incidence of renal tubular necrosis is documented in severe P. falciparum malaria with acute kidney injury, and is associated with the occurrence of apoptosis and expression of cleaved caspase 3. PMID: 26729581
  60. The aim of the study was to evaluate the caspase-3 and survivin expression in correlation with MIB-1 expression in gliomas of various grade. PMID: 26995334
  61. The variant genotypes (CT + TT) of the CASP3 rs12108497 were associated with a significant increased risk of HCC. PMID: 26758428
  62. Specific activation of caspase-3 and caspase-4 was found in proplatelets. Consistent with previous observations of caspase-4 autoactivation in response to endoplasmic reticulum (ER) stress, several ER stress marker proteins were expressed during proplatelet formation. PMID: 27296088
  63. metabolic stress induced dysfunction of the islet beta-cell may, in part, be due to defective protein prenylation signaling pathway. PMID: 27802439
  64. EDA-A2 and its receptor XEDAR are overexpressed in epithelial cells of salivary glands in Sjogren's syndrome patients, in comparison with healthy individuals. The EDA-A2/XEDAR system in these cells is involved in the induction of apoptosis via CASP3 activation. PMID: 26659383
  65. Copper induces the activation of apoptosis through casp3/casp8/casp9/AIF/TP53 signaling pathways. Suggest that apoptosis activation mechanism is dependent on the concentration, time of exposure to Cu and cell type. PMID: 27046389
  66. Thymoquinone-triggered apoptosis was substantiated by up-regulation of the executioner caspase-3 and caspase-7, as well as cleavage of the death substrate poly(ADP-ribose)polymerase. PMID: 27084536
  67. Acetaminophen in a concentration of 10 mM did not induce expression of HIF1A and CASP3, but reduced expression of chaperone HSP90, which attested to activation of a caspase-3-independent mechanism of cell death. PMID: 27165078
  68. Significantly poor overall survival was also reflected by patients with higher fold decrease in CASP3 gene expression. PMID: 26768747
  69. Increased serum caspase-3 activities are highly associated with the severity and prognosis after aneurysmal subarachnoid hemorrhage. PMID: 27380999
  70. Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T2 MR Imaging of Tumor Apoptosis. PMID: 27031226
  71. These findings demonstrated that p21 plays a critical role in furazolidone-induced apoptosis in HepG2 cells by influencing the caspase-3 activation and ROS generation. PMID: 26687534
  72. Expression of livin, survivin and caspase-3 are closely related to the occurrence and development of prostatic cancer. PMID: 26823716
  73. The casp3 cleaved product of Beclin1 promotes apoptosis induced by S1 in SKOV3 cells. PMID: 26573276
  74. These results suggest that CASP3 gene polymorphisms may be associated with susceptibility to periodontal disease in the Korean population PMID: 26782454
  75. Excessive TLR9 signaling contributed to maternal-fetal tolerance disruption via an effect on Treg cell survival by activation of Caspase 8/3 PMID: 26563540
  76. ilvestrol induced caspase-3 activation and apoptotic cell death in a time- and dose-dependent manner PMID: 26762417
  77. Cleaved caspase-3 expression was a predictor of biochemical progression in prostate cancer patients. PMID: 26507126
  78. In addition to acting as a key executioner in apoptosis, caspase 3 in dying tumor cells may play a central role in driving proangiogenic response after irradiation. PMID: 26431328
  79. Caspase-3 mainly degraded LMP1 proteins in HeLa cells, leading to decreased NF-kappaB and STAT3 activation. Caspase-3 cleaved the consensus DNTD sequences in the CTAR3 region of LMP1. PMID: 26921582
  80. in vitro OGT knockdown induced more cell apoptosis through increasing PUMA and caspase-3 expression PMID: 26397041
  81. increased production of vascular endothelial growth factor by mesenchymal stromal cells facilitating AKT phosphorylation and inhibiting caspase 3-mediated apoptosis PMID: 26076727
  82. FANCJ protein is important for the stability of FANCD2/FANCI proteins and protects them from proteasome and caspase-3 dependent degradation. PMID: 26336824
  83. Caspase-3-enhanced Bid activation initiates the genistein induced cell death pathway. PMID: 26469967
  84. Low expression of CASP3 is associated with non-small cell lung cancer. PMID: 26307684
  85. HER2 promotes tumor cell survival by inhibiting apoptosis i.e. by downregulating CASP-3 and CASP-8. PMID: 25189649
  86. Suggest 2'5'-oligoadenylate synthetase 2 mediates T-cell receptor CD3-zeta chain down-regulation via caspase-3 activation in oral cancer. PMID: 26595239
  87. Expression of Caspase 3, including that of survivin, was significantly more frequently detected in the patients who had received combined cytotoxic and anti-VEGF therapy as compared to both those treated with only cytotoxic agents and the control group PMID: 26978233
  88. We identified several genes (FasL, MSH2, ABCC5, CASP3, and CYP3A4)that showed association with PFS in patients with osteosarcoma. These pharmacogenetic risk factors might be useful to predict treatment outcome PMID: 25829401
  89. caspase-3 serum levels and mortality are associated in patients with severe traumatic brain injury PMID: 26545730
  90. overexpression of miR-30b can improve the Hcy-induced apoptosis in HCAECs by downregulating caspase-3 expression. Therefore, miR-30b may play an important role in Hcy-induced apoptosis in endothelial cells PMID: 26263983
  91. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
  92. Deficiency of caspase 3 in tumor xenograft impairs therapeutic effect of measles virus Edmoston strain. PMID: 25909216
  93. these data support a role for the activation of caspase-3 and proteolytic cleavage of Tau antibody in vascular dementia. PMID: 26161867
  94. Nonpeptidyl inhibitors of caspase-3 identified in the present study expeditiously mimic the inhibitory action of the previously identified peptidyl inhibitors PMID: 26064904
  95. The purpose of this study was to comprehensively investigate the association between common genetic variants of CASP3 gene and AF in Chinese Han population. PMID: 25662045
  96. a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-kappaB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3 PMID: 26526615
  97. In patients with HIV, serum CASP3 levels in a group with high CD4 cell counts increased rapidly then decreased within a short time during early HIV-1 infection. In contrast, CASP3 levels in the CD4Low group were increased after 1 year of HIV-1 infection. PMID: 25806508
  98. this report suggests a novel finding that cellular signaling caspase 3/7-PKCdelta-Akt/p38 MAPK is crucial to the repopulation in Panc1 cells after radiotherapy. PMID: 25156550
  99. Targeting executioner procaspase-3 with the procaspase-activating compound B-PAC-1 induces apoptosis in multiple myeloma cells. PMID: 26257207
  100. Expression of caspase-3 in advanced gastric cancer is a predictor of poor response to treatment and survival. PMID: 25476481

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

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