Custom rpoE Antibody

^{Rare Species}

Datasheet

Code	CSB-PA358217XA01SXB
Size	US$166
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Image	Western Blot Positive WB detected in: recombinant protein All lanes:Custom rpoE Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Custom rpoE Antibody Polyclonal antibody

Uniprot No.

P0A2F0

Target Names

Custom rpoE Antibody

Alternative Names

ECF RNA polymerase sigma-E factor (RNA polymerase sigma-E factor) (Sigma-24), rpoE, sigE

Raised in

Rabbit

Species Reactivity

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Immunogen

Recombinant Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Custom rpoE protein (1-191aa)

Immunogen Species

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as acid stress, heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment.

Gene References into Functions

this is the first report demonstrating that RpoE acts as a regulator in the expression of Agf in Salmonella. PMID: 23159630
the extracytoplasmic sigma factor sigma(E) (RpoE) is required for Salmonella resistance to killing by the bactericidal/permeability-increasing protein (BPI)-derived peptide P2 and the murine alpha-defensin cryptdin-4 PMID: 15819632
sigma(E) augments sigma(S) levels during stationary phase via enhanced expression of sigma(H) and the RNA-binding protein Hfq, leading to increased expression of sigma(S)-dependent genes and enhanced resistance to oxidative stress. PMID: 15819634
Nucleotides critical for the activity of the rpoEp3 promoter are identified. PMID: 16842358

Subcellular Location

Cytoplasm.

Protein Families

Sigma-70 factor family, ECF subfamily

Database Links

KEGG: stm:STM2640

STRING: 99287.STM2640

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Custom rpoE Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background