Recombinant Rat Prolyl endopeptidase (Prep)

Code CSB-YP018664RA
MSDS
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Source Yeast
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Code CSB-EP018664RA
MSDS
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Source E.coli
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Code CSB-EP018664RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018664RA
MSDS
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Source Baculovirus
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Code CSB-MP018664RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prep
Uniprot No.
Alternative Names
Prep; Prolyl endopeptidase; PE; EC 3.4.21.26; Post-proline cleaving enzyme; rPop
Species
Rattus norvegicus (Rat)
Expression Region
1-710
Target Protein Sequence
MLSFQYPDVY RDETSVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL YVQDSLEGEA RVFLDPNTLS DDGTVALRGY AFSEDGEYFA YGLSASGSDW VTIKFMKVDG AKELPDVLER VKFTCMAWTH DGKGMFYNSY PQQDGKSDGT ETSTNLHQKL CYHVLGTDQS EDVLCAEFPD EPKWMGGAEL SDDGRYVLLS IWEGCDPVNR LWYCDLQQGS NGINGILKWV KLIDNFEGEY DYITNEGTVF TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV LCYLRNVKNI LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS FLSPGVIYHC DLTREELEPR VFREVTVKGI DASDYQTIQV FYPSKDGTKI PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTTSKRLTI NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG CSDSKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GPGKPTAKVI EEVSDMFAFI ARCLNIEWIQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA.
Gene References into Functions
  1. Frontal cortex prolyl endopeptidase levels are reduced following benzyloxycarbonyl-methionyl-2(S)-cyanopyrrolidine treatment in a rat model of depressive syndrome. PMID: 24288747
  2. PREP is not significantly responsible for the in vivo extracellular space cleavage of substance P or neurotensin. PMID: 21820035
  3. Data show that enzymatic activity of prolyl oligopeptidase (POP) is highest on embryonic day 18 while the protein amounts reach their peak at birth, and support a role for POP in neurogenesis. PMID: 21160163
  4. Overall, the present data support the possibility that PEP exerts specific functions related to neurodevelopment besides those proposed to date. PMID: 20304043
  5. The regional distribution of Prep in rat brain was more homogeneous than in humans, and higher than in peripheral tissues. PMID: 11792464
  6. Prolyl endopeptidase is actually involved in the inactivation of regulatory neuropeptides. PMID: 14986307
  7. Prolyl oligopeptidase is the main enzyme responsible for synthesis of the antifibrotic peptide Ac-SDKP. PMID: 15037553
  8. PTZ-induced seizures of the rats activate glutamate NMDA receptors, then decrease prolyl oligopeptidase enzyme activity in hippocampal neurons. PMID: 15985312
  9. Results describe the distribution of immunoreactive prolyl oligopeptidase in human and rat brain. PMID: 17401647
  10. localization of POP in thalamocortical & corticothalamic projection neurons in ventrobasal complex & medial geniculate nucleus of thalamus & somatosensory/motor & auditory cortices; in thalamus & cortex over 50% of projection neurons contained POP PMID: 19041368

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase S9A family
Tissue Specificity
Expressed in all tissues tested: uterus, kidney, heart, lung, small intestine, smooth muscle, liver, spleen, thymus, adrenal, pituitary and whole brain.
Database Links

KEGG: rno:83471

UniGene: Rn.11058

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