CD21 Antibody

Datasheet

Code CSB-RA005934A0HU
Size US$350
Uniprot No. P20023
Abbreviation Complement receptor type 2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western Blot
    Positive WB detected in:Raji whole cell lysate
    All lanes:CD21 antibody at 0.55ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 155 KDa
    Observed band size: 155 KDa

  • IHC image of CY5710 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA005934A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Raji cells with CSB-RA005934A0HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Overlay histogram showing Raji cells stained with CSB-RA005934A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunogen A synthesized peptide
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200
Relevance Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor, CD21, CR2, C3DR
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Research Area Immunology
Gene Names CR2
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Function Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU
Involvement in disease Systemic lupus erythematosus 9 (SLEB9); Immunodeficiency, common variable, 7 (CVID7)
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Receptors of complement activation (RCA) family
Tissue Specificity Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Database Links

HGNC: 2336

OMIM: 120650

KEGG: hsa:1380

UniGene: Hs.445757

Pathway B cell receptor signaling pathway
Complement and coagulation cascades
Hematopoietic cell lineage

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