GLP1R

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R at 2 μg/mL can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA1HU), the EC₅₀ is 54.54-94.23 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The Binding Activity of GLP1R with anti-GLP1R antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R(CSB-MP009514HUb1) at 2 μg/mL can bind Anti-GLP1R recombinant antibody, the EC₅₀ is 54.54-94.23 ng/mL.

Untransfected HEK-293T cells surface (green line) and transfected Human GLP1R HEK-293T stable cells surface (red line) were stained with anti-GLP1R antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Positive WB detected in: MCF-7 whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Rat Heart tissue lysate(30µg)
All lanes: GLP1R antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 300s

Western blot
All lanes: GLP1R antibody at 12µg/ml
Lane 1: Mouse heart tissue
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

Immunofluorescent analysis of 293T cells using CSB-PA009514YA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)