Recombinant Mouse Tyrosine-protein kinase Lyn(Lyn)

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Code CSB-EP013259MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Lyn
Uniprot No. P25911
Research Area Signal Transduction
Alternative Names Lyn; Tyrosine-protein kinase Lyn; EC 2.7.10.2; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn
Species Mus musculus (Mouse)
Source E.coli
Expression Region 2-512aa
Target Protein Sequence GCIKSKRKDNLNDDEVDSKTQPVRNTDRTIYVRDPTSNKQQRPVPEFHLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLSSKREGFIPSNYVAKVNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDYDPMHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQSDGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKKLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTKEEPIYIITEFMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMSALSQGYRMPRMENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 63.7 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3-kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation.
Gene References into Functions
  1. Fyn and Lyn as important factors that promote Plasmacytoid dendritic cell responses. PMID: 28368000
  2. these findings demonstrated that Lyn overexpression ameliorated airway mucus hypersecretion by down-regulating STAT6 and its binding to the MUC5AC promoter. PMID: 28205598
  3. A novel pancancer mechanism of Lyn-dependent control of epithelial-mesenchymal transition and role of this kinase in tumor progression. PMID: 28288135
  4. these results reveal a role for Lyn as a specific suppressor of the TLR-MyD88-IRF5 pathway and illustrate the importance of fine-tuning IRF5 activity for the maintenance of immune homeostasis PMID: 27521268
  5. These findings reveal that the function and regulation of Lyn during B1 cell B cell receptor signaling is distinct from other B cell subsets. PMID: 27889108
  6. A mutation in LYN, an inhibitory protein tyrosine kinase that is implicated in systemic autoimmunity, combines with an Aire mutation to provoke organ-specific autoimmunity. PMID: 27571405
  7. the Hck, Fgr and Lyn kinases are also necessary for amastigote uptake by macrophages. Src-mediated Arg activation is required for efficient uptake. PMID: 27358479
  8. the Lyn mutation in NOD neutrophils is likely responsible for dysregulation of neutrophil adhesion and directed migration. PMID: 27591397
  9. Our studies indicate not only a concept of mucus hypersecretion in asthma that involves Lyn kinase but also an important therapeutic candidate for asthma. PMID: 28024734
  10. Together, the basal-state signaling checkpoint regulated by LynA expression and degradation and the signaling reorganization initiated by receptor clustering allow cells to discriminate optimally between pathogens and nonpathogens. PMID: 26517880
  11. Oxidized LDL-bound CD36 recruits an Na/K-ATPase-Lyn complex in macrophages that promotes atherosclerosis. PMID: 26350901
  12. Lyn kinase modulates inhibitory signaling to suppress endotoxin-induced lung inflammation. PMID: 26453518
  13. A complex functional interplay between FcgammaRIIa, Lyn, and alphaIIbbeta3 in immune complex-induced platelet activation. PMID: 26291522
  14. report that Lyn is required for bacterial infection-induced recruitment of autophagic components to pathogen-containing phagosomes PMID: 26735693
  15. Data indicate interactions between Ets1 transcription factor and two of its important regulators: Lyn, which maintains Ets1 expression to limit the differentiation of autoreactive plasma cells (PCs), and Btk. PMID: 26209625
  16. results indicate that Lyn plays a positive regulatory role in RIG-I-mediated interferon expression as a downstream component of IPS-1 PMID: 25585356
  17. Fyn, but not Lyn, was required for complete Pyk2 phosphorylation by thrombin. PMID: 25967238
  18. Data show that tyrosine protein kinase Lyn acts as a sensor of hydrogen peroxide (H2O2) levels to facilitate microglial migration toward alpha-Synuclein (alpha-syn). PMID: 25825709
  19. Lyn is a key regulator of survival signaling in plasma cells, limiting plasma cell accumulation and autoimmune disease susceptibility. PMID: 25118329
  20. Lyn is a key regulator of the mucosal immune system, governing pathophysiology in multiple models of intestinal disease. PMID: 25339668
  21. The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment. PMID: 25225462
  22. Lyn activity protects mice from DSS colitis and regulates the production of IL-22 from innate lymphoid cells. PMID: 24045577
  23. analyses revealed a SHP2- and Lyn-dependent pathway leading to phosphorylation of Vav1, Rac activation, and F-actin polymerization in SCF-treated BMMCs PMID: 24733849
  24. Data (including data from mutant mice) suggest Lyn facilitates erythrocyte production/erythropoiesis by influencing different stages of erythroid progenitor expansion, differentiation, mature cell development, cell survival, and signal transduction. PMID: 24552351
  25. Data suggest that the tyrosine phosphorylation profile of Lyn in B-lymphocytes is altered in mercury poisoning; Lyn appears to play role as pivotal mediator of mercury immunotoxicity; thus, Lyn is a potential biomarker for mercury exposure. PMID: 24440445
  26. Lyn regulates inflammatory responses in Klebsiella pneumoniae infection via the p38/NF-kappaB pathway. PMID: 24338528
  27. the TAG-1-mediated cell-to-cell interaction between the unpolarized multipolar cells and the pioneering axons regulates the polarization of multipolar cells partly through Lyn kinase and Rac1 PMID: 24559674
  28. B cell-intrinsic Lyn-dependent signaling pathways regulate B cell homeostasis and activation, which in concert with B cell-specific MyD88 signaling pathways can drive the development of autoimmune disease. PMID: 24376269
  29. regulates TGF-beta3 isoform and modulates the development of airway remodeling PMID: 24127553
  30. in contrast to c-Src and Yes, which increase vascular permeability in response to stimuli, Lyn stabilizes endothelial junctions through phosphorylation of FAK. PMID: 24108461
  31. Lyn gene dosage and activity are critical for normal erythropoiesis; constitutively active Lyn alters Epo signaling, which in turn produces erythroid defects. PMID: 23692855
  32. Data show that the hyperresponsive phenotype of B cells lacking Lyn is predicated on significantly increased basal and inducible PI3K activity. PMID: 22777522
  33. Chronic inflammation can induce thymic atrophy and perturb spleen homeostasis in LynDeltaN mice through the increased production of TNFalpha, LTss and TNFR1 signaling. PMID: 23071785
  34. these results indicate that Lyn plays a positive role in TLR4-induced production of TNF-alpha in MCs controlling the activity of the TRAF-6/TAK-1 protein complex. PMID: 22302035
  35. MLR-1023 increased the V(max) of Lyn with an EC(50) of 63 nM. PMID: 22473614
  36. Lyn kinase activator MLR-1023-mediated blood glucose lowering was insulin-dependent. PMID: 22431203
  37. Lyn deficiency disturbs the generation of mature germinal center (GC) B cells and leads to impaired development of mature GCs. PMID: 22942428
  38. This study demonstrates that Lyn is a haploinsufficient gene in autoimmune disease PMID: 22798664
  39. Lyn enhances endotoxin-induced dendritic cell (DC) maturation and modified DC cytokine expression profiles, leading to elevated natural killer (NK) cell IFN-gamma production. PMID: 22491248
  40. Lyn but not Fyn kinase controls IgG-mediated systemic anaphylaxis. PMID: 22450804
  41. The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells PMID: 22246800
  42. Lyn-deficient T2 and follicular B cells expressed elevated levels of the pro-apoptotic factor Bim. PMID: 21928281
  43. protein deficiency affects B-cell maturation as well as survival PMID: 22057631
  44. A model in which PECAM-1/SHP-2 complexes, formed in a Lyn-dependent manner, suppress GPVI signaling. PMID: 21297004
  45. Despite possessing enhanced killing, alveolar macrophage Lyn/Fgr/Hck-deficient (triple-knockout) mice do not demonstrate enhanced inflammatory responses to Pneumocystis murina. PMID: 21220696
  46. The results of this study suggested a novel role for Lyn kinase in the regulation of DA release in the mesolimbic system, which leads to the control of alcohol reward. PMID: 21307254
  47. Lyn- and PLC-beta3-dependent regulation of SHP-1 phosphorylation controls Stat5 activity and myelomonocytic leukemia-like disease PMID: 20858858
  48. Although Lyn is involved in dampening the cytokine Toll-like receptor response, Lyn-deficient macrophages and mice still develop microbial tolerance. PMID: 20385881
  49. Lyn B isozyme differs from Lyn A in its association with negative regulatory lipid phosphatase SHIP-1 and in the regulation of calcium responses. PMID: 20308635
  50. Lyn stimulates platelet secretion by activating the phosphoinositide 3-kinase-Akt-nitric oxide (NO)-cyclic GMP pathway and also provide an explanation why Lyn can both stimulate and inhibit platelet activation PMID: 20189992
  51. Although Lyn-deficient B cells have the capacity to produce class-switched, pathogenic autoantibodies, it is the inflammatory environment in which the B cells reside that determines their propensity to do so. PMID: 20042579
  52. the GPVI receptor utilizes a unique intracellular proline-rich domain (PRD) to accelerate platelet activation, a requirement for efficient platelet adhesion to collagen under flow PMID: 19940238
  53. The Lyn/CD22/SHP-1 pathway is important in autoimmunity. Naive and tolerant B-cells differ in their calcium signaling in response to antigenic stimulation. PMID: 11826756
  54. The tyrosine kinase Lyn is required for B cell development beyond the T1 stage in the spleen. Lyn plays a positive role in selection and survival of mature B cells in addition to its previously documented negative role in tolerance and B cell activation. PMID: 11920569
  55. data indicate that ligation of Fcgamma receptors activates Lyn followed by Syk kinase during phagocytosis PMID: 12056610
  56. First high-resolution immunogold observation of the sequential engagement of Lyn kinase in phagocytosis mediated by Fc gamma receptors. PMID: 12471110
  57. The regulatory activity of Lyn induced by B cell receptor stimulation is unaffected by the absence of CD19. PMID: 12471124
  58. Data describe the pivotal role of Lyn in B cell signaling, and that sustained activation of Lyn tyrosine kinase in vivo leads to autoimmunity. PMID: 12486102
  59. First direct evidence is given that reveals a negative role for Lyn in the regulation of integrin-mediated signaling in murine bone marrow neutrophils and macrophages. PMID: 12874221
  60. Hyperresonsiveness to B cell receptor cross-linking is insufficient for autoantibody production, B-1 cell expansion, and splenomegaly in lyn-/- mice. PMID: 12902486
  61. Lyn is a negative regulator of murine bone marrow-derived mast cell survival and proliferation. PMID: 14525964
  62. data propose that Lyn, not Jak2, phosphorylates Gab2 and that maximal phosphorylation of Gab2 requires Y764, a Grb2-binding site. PMID: 14656892
  63. Mpl stimulation results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
  64. The presence of Lyn regulates PU.1 expression and PU.1-dependent activity of the germinal center-associated nuclear protein (GANP) promoter via a novel B-cell signaling pathway: Lyn-->PU.1-->GANP. PMID: 15034025
  65. Lyn functions as a negative regulator of FcepsilonRI-mediated signaling in mast cells. PMID: 15210764
  66. Lyn may negatively regulate the PI 3 Kinase/Akt pathway. The opposing effects of Lyn on these signaling pathways may explain the pleiotropic effects ascribed to this Src family member in the literature. PMID: 15451030
  67. These data suggest that the Na+, K+-ATPase activity in central neurons is regulated by specific Src tyrosine kinases via a protein-protein mechanism and may play a role in apoptosis. PMID: 15935067
  68. Lyn regulates the intensity and duration of multiple asthmatic traits PMID: 16034130
  69. Lyn kinase plays uniquely negative and positive regulatory roles in DC generation and maturation, respectively. PMID: 16116174
  70. upon low intensity stimulation of FcepsilonRI with monomeric IgE, IgE plus anti-IgE, or IgE plus low Ag, Lyn (a Src family kinase) positively regulates degranulation, cytokine production, and survival PMID: 16272347
  71. microarray data identify Lyn as a negative regulator in Ag-stimulated bone marrow-derived mast cells of the expression of genes linked to FcepsilonRI signaling and also to the response pathways that lead to allergy and asthma PMID: 16339523
  72. Lyn kinase is a negative regulator of thrombopoietin signaling. PMID: 16418722
  73. Results show that the SH2 domain of Lyn is responsible for the accelerated induction of neutrophilic nuclear lobulation upon G-CSF stimulation. PMID: 16616866
  74. Numerous studies have implicated Lyn in growth factor receptor signal amplification, while a number also suggest that Lyn participates in negative regulation of growth factor signaling. PMID: 16801133
  75. a single phosphotyrosine residue on Cbp coordinates a two-phase process involving distinct negative regulatory pathways to inactivate, then degrade, Lyn PMID: 16920712
  76. Activation of both Lyn and PI3-kinase was required for enhancing paxillin phosphorylation. PMID: 17192284
  77. Hic-5 and paxillin function as negative feedback regulators of Src family kinases in aggregated platelets PMID: 17233630
  78. Lyn activity is elevated in hck(-/-) cells, leading to increased phosphorylation of several negative regulators PMID: 17513616
  79. A novel phagocytosis pathway for Pseudomonas aeruginosa is initiated by cholesterol-rich membrane rafts and is regulated by Lyn, which is primarily an immune regulator with both positive and negative roles. PMID: 18250449
  80. Taken together, our results demonstrate an essential role for Lyn kinase in positively regulating the long-term and multilineage engraftment of stem cells, which is distinct from its role in mature B cells and myeloid cells. PMID: 18346837
  81. important role for Lyn in VWF/GPIb-IX-induced integrin activation mediated via the cGMP signaling pathway independently of TXA2 synthesis and also indicate that Lyn is critically important in GPIb-IX-mediated activation of the cGMP pathway PMID: 18550847
  82. Visualization and analysis of the earliest events in BCR signaling highlight the importance of the membrane microenvironment for formation of BCR-Lyn complexes and the B cell immune synapse. PMID: 18644892
  83. Integrin-associated Lyn kinase acts as a key upstream kinase that may play a critical role in cerebral ischemia/reperfusion injury PMID: 18682390
  84. Crystal structures of the Lyn protein tyrosine kinase domain in its Apo- and inhibitor-bound state. PMID: 18984583
  85. Liar is a novel Lyn-interacting molecule that plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. PMID: 19064729
  86. This demonstrates the physiological importance of the conserved G loop salt bridge and at the same time distinguishes the in vivo requirement for the Lyn kinase activity from other potential functions of the protein. PMID: 19150426
  87. Lyn Src family kinase suppresses osteoclastogenesis in vitro and in vivo PMID: 19171907
  88. an additional role for Lyn kinase, distinct from its catalytic activity, in regulating intracellular signaling pathways PMID: 19201855
  89. of Lyn caused a striking increase in the RANKL-induced PLCgamma1, Ca(2+), and NFATc1 responses during differentiation. PMID: 19285079
  90. basophil-expressed Lyn kinase exerts regulatory control on Th2 cell differentiation and function PMID: 19362019
  91. Inhibition of B cell receptor signaling by the Lyn kinase-CD22 antigen-Src homology region 2 domain-containing phosphatase-1 (SHP-1) pathway is up-regulated as splenic B cells mature, and is involved in maintenance of peripheral B-cell immune tolerance. PMID: 19380785
  92. Findings suggest a defect in CD19-mediated signals in both peritoneal and splenic B-1 B lymphocytes, which is in part, due to higher levels of constitutively active Lyn. PMID: 19541367
  93. Expression of the caspase-cleaved form of Lyn (LynDeltaN), in mice, mediates a chronic inflammatory syndrome resembling human psoriasis. PMID: 19590497
  94. both positive and negative regulatory functions of Lyn are predominantly carried out by its catalytic activity and not an adaptor function. PMID: 19949072

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Subcellular Location Cell membrane, Nucleus, Cytoplasm, Cytoplasm, perinuclear region, Golgi apparatus, Membrane, Lipid-anchor
Protein Families Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
Tissue Specificity Detected in bone marrow-derived monocytes (at protein level). Expressed predominantly in B-lymphoid and myeloid cells.
Database Links

KEGG: mmu:17096

STRING: 10090.ENSMUSP00000038838

UniGene: Mm.317331

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